Zootaxa 3926 (4): 499–522 ISSN 1175-5326 (print edition) Article ZOOTAXA www.mapress.com/zootaxa/ Copyright © 2015 Magnolia Press ISSN 1175-5334 (online edition) http://dx.doi.org/10.11646/zootaxa.3926.4.3 http://zoobank.org/urn:lsid:zoobank.org:pub:8C078FEF-60FC-41A1-A305-E0DBA2B8DAF5 A review of Mimapsilopa Cresson (Diptera: Ephydridae) from Brazil WAYNE N. MATHIS1, DANIEL N. R. COSTA2 & LUCIANE MARINONI2 1Department of Entomology, NHB 169, PO Box 37012, Smithsonian Institution, Washington, D. C. 20013-7012, United States. E-mail: [email protected]. 2Departamento de Zoologia, Universidade Federal do Paraná, Jardim das Américas, 81531-980 - Curitiba, Paraná, Brazil. E-mail: [email protected] and [email protected] Abstract Species of Mimapsilopa from Brazil are reviewed with an emphasis on the fauna from southern Brazil, where six new species were discovered and herein are diagnosed and described. To facilitate identification of species, we have included a diagnosis of the tribe Discomyzini and of Mimapsilopa and have also provided an annotated key to the New World gen- era of the tribe. We have also provided photos of representative specimens, illustrations of structures of the male terminalia and a distribution map for all included species. Key words: Neotropical Region, shore flies, taxonomy Introduction The little-known shore-fly genus Mimapsilopa Cresson is known to occur only in the New World (Mathis and Zatwarnicki 1995), primarily in tropical zones. Mimapsilopa has been treated infrequently, even among shore-fly workers, and its relationship with other genera within the tribe Discomyzini remains largely unresolved (Mathis and Zatwarnicki 1998). Cresson (1941), for example, proposed Mimapsilopa, but five years later he (Cresson 1946) treated the included species in Helaeomyia, a second genus Cresson also described in the same 1941 paper but a page earlier. Mimapsilopa was considered a junior synonym of Helaeomyia (Cresson 1946, Wirth 1968) until Lizarralde de Grosso (1982) revised the species related to Helaeomyia and included recognition of Mimapsilopa as a separate genus. Lizarralde de Grosso's revision comprised six species, four being newly described. In our world catalog (Mathis and Zatwarnicki 1995), we followed Lizarralde de Grosso's precedent and recognized Helaeomyia and Mimapsilopa as separate genera. Our listing in the catalog was based on Zatwarnicki's research, especially on structures of the male terminalia that indicates the species placed in these two genera form monophyletic and separate lineages. Since publication of the world catalog, Mathis and Zatwarnicki (1998) added two species from the West Indies, resulting in a total of nine congeners in Mimapsilopa before this paper. The species of Mimapsilopa occurring in Brazil are the subject of this review, and all six of the included species are new and are described herein. This review of Mimapsilopa was prompted by recent field work in southern Brazil that is part of an overall survey of the shore flies of this biologically diverse country. An objective of the field work in 2009–2010 was the shore-fly fauna from the state of Paraná and to a lesser degree from Santa Catarina and São Paulo and resulted in numerous specimens of Discomyzini, including Mimapsilopa. We soon discovered that Mimapsilopa is represented by six undescribed species from Brazil. The purpose of this paper is to describe these new species within the context of a faunistic review of Mimapsilopa from Brazil. All six species are described, including structures of the male terminalia, which are also illustrated. Accepted by S. Gaimari: 11 Feb. 2015; published: 9 Mar. 2015 499 Licensed under a Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0 Material and methods The descriptive terminology, with the exceptions noted in Mathis (1986) and Mathis & Zatwarnicki (1990a), follows that published in the Manual of Nearctic Diptera (McAlpine 1981). Because specimens of Discomyzini are generally moderately small, usually less than 4.0 mm in length, study and illustration of the male terminalia required use of a compound microscope. We have followed the terminology for most structures of the male terminalia that other workers in Ephydridae have used (see references in Mathis 1986, and Mathis & Zatwarnicki 1990a, 1990b), such as surstylus. Zatwarnicki (1996) suggested that the pre- and postsurstylus correspond with the pre- and postgonostylus and that the subepandrial plate is the same as the medandrium. The terminology for structures of the male terminalia is provided directly on Figs. 4–7. Dissections of male and female genitalia and descriptions were performed using the method of Clausen & Cook (1971) and Grimaldi (1987). Microforceps were used to remove abdomens, which were macerated in a potassium hydroxide solution. Cleared genitalia were rinsed in a weak solution of acetic acid and then transferred to glycerin for observation and illustration. If necessary for proper orientation, the specimen was transferred from glycerin to glycerin jelly. The glycerin jelly was heated, and the specimen appropriately oriented. After cooling, the embedded specimen in glycerin jelly became immobilized. The abdomen was placed in a plastic microvial filled with glycerin and attached to the pin supporting the remainder of the insect from which it was removed. Illustrations of structures of the male terminalia were produced by first producing a pencil illustration through a camera lucida on a compound microscope, which was then rendered as a vector file in ©Adobe Illustrator. The resulting illustration, as a vector file, was then compared again with the structure for accuracy. Photographs of wings and heads, especially faces, were taken with a Leica DFC 500 digital camera attached to a stereoscopic microscope (Leica MZ 16). The images were captured with the software Combine ZP from the Project Taxon line—Network of Biological Collections of Paraná State. The images series obtained were combined by Zerene Stacker and retouched with ©Adobe Photoshop. Distribution map were made using ESRI ArcView GIS 3.2. The new species descriptions are composite and not based solely on holotypes. One head and two venational ratios used in the descriptions are based on three specimens (largest, smallest, and one other): Eye ratio: maximum width/maximum height; gena-to-eye ratio: genal height (immediately below maximum eye height)/eye height; costal vein ratio: the straight line distance between the apices of R and R /distance between the apices of R and 2+3 4+5 1 R ; M vein ratio: the straight line distance along vein M between crossveins dm-cu and r-m/distance apicad of 2+3 dm-cu. Specimens for this study are in the Natural History Museum, London (BMNH); Universidade Federal do Paraná (DZUP: Coleção Entomológica Padre Jesus Santiago Moure, Departamento de Zoologia, Curitiba, Paraná, Brazil); Museu de Zoologia da Universidade de São Paulo, São Paulo, Brazil (MZUSP); and the National Museum of Natural History, Smithsonian Institution, Washington, D. C. (USNM). Taxonomy Tribe Discomyzini Acloque (12 genera, 56 species) Discomyzini Acloque 1897: 486. Type genus: Discomyza Meigen 1830.─Mathis and Zatwarnicki 1995: 21–30 [world catalog]. Diagnosis. The tribe Discomyzini is distinguished from other shore-fly tribes by the following combination of characters: Small to moderately small shore flies, body length 1.50–4.50 mm; body color and vestiture quite variable, from mostly black and shiny with sparse vestiture of microtomentum to yellowish red and densely invested with microtomentum. Head: Ocellar seta aligned behind anterior ocellus, sometimes only slightly so; pseudopostocellar seta small (elongate in Clasiopella Hendel); fronto-orbital setae reclinate and/or proclinate; reclinate fronto-orbital seta usually inserted behind larger, proclinate fronto-orbital seta. Pedicel bearing an elongate, conspicuous, dorsal, spine-like seta. Facial conformation variable, smooth to strongly and coarsely sculptured on at least lower 1/2; medial facial setae short, the longest at most as long as its distance from opposite seta; facial area and ventral facial margin without setae; facial setae inserted in more or less vertical series, parallel 500 · Zootaxa 3926 (4) © 2015 Magnolia Press MATHIS ET AL. with parafacial; subcranial cavity small to large. Thorax: Prescutellar acrostichal setae usually present, large (subequal to posterior dorsocentral seta), inserted widely apart (distance between setae subequal to that between either prescutellar and the posterior dorsocentral seta on the same side) and usually inserted anterior of intra-alar seta; presutural or sutural dorsocentral and postsutural setae absent (a synapomorphy for Discomyzinae). Base or stem of R bearing 2–4 setulae on dorsum (lacking secondarily in Rhysophora and Guttipsilopa Wirth). Abdomen: 4+5 Male terminalia: Presurstylus well developed; postsurstylus (clasper) lobate, generally setose, sometimes with a postsurstylar process; subepandrial plate present; pregonite moderately well developed, usually bearing 2–3 long, apical setulae(a synapomorphy for Discomyzinae); postgonite often greatly reduced, lacking, or perhaps fused with pregonite(a synapomorphy for Discomyzinae); hypandrium round; aedeagus tubular, simple; phallapodeme generally hemispherical to rounded triangular in lateral view; ejaculatory apodeme lacking(a synapomorphy for Discomyzinae). Female terminalia: Ventral receptacle with a relatively large operculum and curved extended process. Larva: Anterior spiracle cauliflower shaped. Discussion. We follow Zatwarnicki (1992) in recognizing two tribes (Discomyzini and Psilopini) within the subfamily Discomyzinae. Evidence for the monophyly of Discomyzini, however, is not overwhelming (base or stem of vein R bearing 2–4 setulae on dorsum) and more evidence and assessment are needed. For example, the 4+5 synapomorphies noted in the tribal diagnosis mostly apply to both Discomyzini and Psilopini (the subfamily Discomyzinae). The tribe Discomyzini presently comprises 12 genera and 56 species (Mathis and Zatwarnicki 1995 and electronic updates). Key to genera of Discomyzini from the New World 1. A postsutural supra-alar seta much reduced (no larger than surrounding setulae) or absent . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 - A postsutural supra-alar seta present, size subequal to presutural supra-alar seta . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 ° 2. Pseudopostocellar setae well developed, length about 1/2 that of ocellar setae, orientation divergent at usually less than 90 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Clasiopella Hendel - Pseudopostocellar setae weakly developed, length considerably less than 1/2 that of ocellar setae, orientation variable. . . . . .3 3. Only the reclinate fronto-orbital seta well developed; face conspicuously and deeply, transversely rugose. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Discomyza Meigen - At least 1 proclinate fronto-orbital seta in addition to reclinate seta well developed; face usually at most with shallowly impressed, transverse striae. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 4. Eye appearing bare; 1 well-developed proclinate fronto-orbital seta (2nd seta greatly reduced), inserted anterior to reclinate seta; presutural supra-alar seta weakly developed, length less than anterior notopleural seta (except in M. cressoni Lizarralde de Grosso); legs bicolored. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Mimapsilopa Cresson - Eye conspicuously setulose; 2 well-developed proclinate fronto-orbital setae, anterior proclinate seta at about same level as large, reclinate seta, posterior proclinate seta inserted posterior of reclinate seta; presutural supra-alar seta well developed, length longer than anterior notopleural seta; legs unicolorous, blackish brown. . . . . . . . . . . . . . . . . . . . . . Helaeomyia Cresson 5. Interfrontal seta present; fronto-orbital setae 4 (anterior 2 setae proclinate, 3rd lateroclinate, 4th lateroreclinate). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Paratissa Coquillett - Interfrontal seta absent; fronto-orbital setae 2–3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6 6. Supra-alar seta well developed, length subequal to postalar seta; wing usually mostly hyaline, at most with anterior margin faintly infuscate. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Rhysophora Cresson - Supra-alar seta moderately well developed, length about half postalar seta; wing usually with at least anterior margin infumate, wing sometimes mostly brown and with white spots . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .Guttipsilopa Wirth Genus Mimapsilopa Cresson Mimapsilopa Cresson 1941: 36. Type species: Clasiopella metatarsata Cresson 1939, original designation.—Cresson 1946: 153 [synonymy with Helaeomyia Cresson].—Lizarralde de Grosso 1982: 121–128 [revision].—Lizarralde de Grosso et al. 2011: 4 [catalog, Argentina].—Mathis and Zatwarnicki 1995: 27–28 [world catalog]; 1998: 7–24 [review of West Indian species]. Diagnosis. Mimapsilopa is distinguished from other genera of Discomyzini by the following combination of characters: Small to medium-sized shore flies, body length 1.30–3.40 mm; generally black species, many surfaces subshiny to shiny. Head: Normally developed, not triangular or with bulging eyes; antenna inserted at dorsal 1/3 of head height; frons conspicuously wider than long, sparsely microtomentose to microgranulose, contrasted from REVIEW OF MIMAPSILOPA FROM BRAZIL Zootaxa 3926 (4) © 2015 Magnolia Press · 501 subshiny to shiny face, vertex distinctly to somewhat angulate, not broadly rounded; interfrontal setae absent; well- developed fronto-orbital setae 2 (2nd proclinate seta greatly reduced, inserted posterior of larger proclinate seta), proclinate seta usually smaller than reclinate seta, inserted anterior of reclinate seta; ocellar setae well developed, inserted behind level of anterior ocellus, orientation usually proclinate and slightly divergent; pseudopostocellar setae weakly developed, divergent and slightly proclinate; both medial and lateral vertical setae well developed, lateral seta shorter than medial seta. Antennal shape quite variable; arista pectinate, bearing 5–11 dorsal rays or hairs. Eye irregularly elliptical, higher than wide, interfacetal setulae sparse, appearing bare. Face swollen medially, mostly to entirely bare, shiny, smooth to microsculptured, lacking pits; well-developed facial setae 2, level of insertion variable, inclinate and usually slightly dorsoclinate; proboscis normally developed, not elongate; palpus black. Thorax: Generally black, mesonotum, including postpronotum and notopleuron sparsely microtomentose, thereafter ventrally, including most of pleural area, mostly bare of microtomentum, shiny black; scutellum more or less triangular, posterior angle bluntly rounded. Chaetotaxy as follows: prescutellar acrostichal setae well developed, inserted far anteriad, slightly anteriad of level of single, large, dorsocentral seta, distance between dorsocentral setae more than that between apical scutellar setae; presutural supra-alar seta variable, well developed or greatly reduced; postsutural supra-alar seta lacking; postalar seta 1; scutellar disc moderately setulose; basal scutellar seta over 1/2 length of apical seta; notopleuron lacking setulae but bearing anterior and posterior setae, these equidistant from notopleural suture; anepisternum with 2 large setae at posterior margin, ventral seta only slightly longer to nearly twice length of dorsal seta; katepisternum with 1 large setae. Halter with knob white to yellowish. Wing variable, hyaline or with pattern of infuscation; vein R extended normally to 2+3 costal margin, well separated from costa, lacking a stump vein, moderately long, making section II about 1.5 length of section III; vein M very shallowly curved anteriorly on apical portion; R stem vein bearing 2–4 setulae dorsally; crossvein dm-cu straight. Tarsi, at least basitarsomere, white to yellow, contrasted sharply from dark colored tibiae and femora, in some species the dorsum of the basitarsomere is somewhat darkened; forefemur with dorsal surface uneven, slightly emarginate. Abdomen: Mostly shiny, blackish, microtomentum generally sparse; tergite 5 of male shinier than preceding tergites, almost devoid of microtomentum, anterior margin with broad, shallow emargination dorsomedially, bearing longer setae along posterior margin. Male terminalia mostly symmetrical; epandrium as an inverted U in posterior view, arms projected ventrad, posterior surface generally setulose, generally thickly formed; cercus in posterior view broadly lunate, especially ventrally, dorsal apex more narrowly pointed; presurstylus large at ventral margin of epandrium, medial surface with a small emargination dorsally, thereafter ventrally on apical 2/3 shallowly concave and bearing numerous, short setulae, external surface arched; postsurstylus elongate, bearing numerous setulae, symmetrical or asymmetrical at apex, apex sometimes bilobed, mediobasal surface of some species bearing an internal, medially directed, usually rod-like postsurstylar process; subepandrial plate broadly U-shaped, base longer than length of arms; aedeagus longer than wide, variously shaped, often quadrate basally in lateral view; phallapodeme in lateral view more or less triangular, angle at attachment with hypandrium thicker; hypandrium in lateral view angulate, usually becoming much wider toward anterior margin, concavity moderately deep, pocket-like. Discussion. Following the precedent of Mathis and Zatwarnicki (1998), we recognize two species groups within Mimapsilopa, and herein, we treat the included species in both species groups in alphabetical order. Key to species of Mimapsilopa from Brazil 1. Wing infuscate, at least along anterior margin or apically (Figs. 46–50). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2 - Wing hyaline (Fig. 51) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6 2. Wing with broad to narrow transverse bands, one subapical, other apical (Figs. 46, 47). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3 - Wing infuscate along anterior margin, becoming gradually hyaline toward posterior margin (Figs. 48–50); face variable but not conspicuously rugose . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4 3. Mid- and hindtibiae yellow; foreleg black except for apical 2–3 yellowish tarsomeres. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M. rugosa Mathis, Costa & Marinoni n.sp. - All tibiae largely black; foreleg, including tarsomeres, black. . . . . . . . . . . . . . . . . . . .M. xingu Mathis, Costa & Marinoni n.sp. 4. Face largely bare, somewhat polished and shiny (Figs. 16–18) . . . . . . . . . . . . . . . . . M. onssa Mathis, Costa & Marinoni n.sp. Face microsculptured to granulose, often with shallow vertical striae (Figs 1–3) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5 5. Basal flagellomere mostly blackish, only slightly yellowish at base margin (Figs. 8–10). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M. iguassu Mathis, Costa & Marinoni n.sp. - Basal flagellomere more extensively yellowish at base and along ventrobasal margin (Figs. 1–3). . . . . . . . . . . . . . . . . . . . . . . . 502 · Zootaxa 3926 (4) © 2015 Magnolia Press MATHIS ET AL. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M. acta Mathis, Costa & Marinoni n.sp. 6. Small species, wing length 1.80 mm or smaller; forebasitarsomere whitish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .M. oligocrada Lizarralde de Grosso [not yet recorded from Brazil] - Larger species, wing length 2.30 mm or larger; forebasitarsomere dark brown. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . M. plaumanni Mathis, Costa & Marinoni n.sp. The cressoni Group Diagnosis. Head: Face finely granulose and with some shallow, transverse rugosity or with conspicuous rugosity; dorsoapical seta of pedicel long, length greater than width of pedicel; basal flagellomere elongate, length about twice or more height. Thorax: Presutural supra-alar seta well developed, length subequal to notopleural setae; ventral anepisternal seta at posterior margin nearly twice length of dorsal seta; dorsal and posterior margins of anepisternum microtomentose, similar to notopleuron. Forecoxa sparsely microtomentose; forebasitarsomere variable, some species yellow, similar to mid- and hindtarsi, other species black, unicolorous with other tarsomeres. Abdomen: Male terminalia: Epandrium with high cercal cavity, making dorsal portion of epandrium narrow; and presurstylus lacking presurstylar basomedial process, with large, medial seta (sometimes 2) and medial surface rounded, not concave; postsurstylus lacking rod-like process; pre- and postgonites fused, elongate. Mimapsilopa acta Mathis, Costa & Marinoni n.sp. Figs. 1–7, 50, 52. Diagnosis. This species is distinguished from congeners by the following combination of characters: Generally very dark brown, shiny; moderately small shore flies, body length 2.15–2.80 mm. Head (Figs. 1–3): Frons dark brown, microsculptured. Basal flagellomere blackish dorsoapically, yellowish basoventrally, gradually tapered to rounded apex; arista with 9–10 dorsal rays. Face mostly shiny bluish black to black, microsculptured with shallow, mostly vertical striae. Eye ratio: 0.62–63; gena-to-eye ratio: 0.12–0.13. Thorax: Mesonotum sparsely microtomentose with brown microtomentum on shiny black background, appearing subshiny; anepisternum mostly shiny, only extreme margins microtomentose. Wing (Fig. 50) mostly infuscate, anterior margin brown, becoming progressively lighter colored posteriorly, posterior margin largely hyaline; length 1.85–2.00 mm; costal ratio 0.61–0.68; M vein ratio 0.77–0.81. Knob of halter white, stem yellowish. Foreleg, including forebasitarsomere and femora, and tibiae of mid- and hindlegs dark brown to black; extreme apex of mid- and hindtibiae and mid- and hindtarsi yellow except for darkened apical tarsomere. Abdomen: Tergites sparsely microtomentose, mostly shiny; tergites 4, 5 about equal in length, both longer than tergite 3; tergite 5 bare, shiny. Male terminalia (Figs. 4–7): Epandrium in posterior view (Fig. 4) setulose with short, stout setulae, shape as an inverted, thick-walled U, widest at dorsolateral corners, slightly thinner at dorsal portion above cercal cavity, in lateral view (Fig. 5) more or less rectangular dorsally, then becoming wider subventrally with anterior, relatively narrow protrusion, ventral margin nearly straight but slanted; cercus in posterior view (Fig. 4) elongate, shallowly lunate but not sharply pointed at ventral and dorsal apices, in lateral view (Fig. 5) semi-hemispherical; presurstylus symmetrical, in posterior view (Fig. 4) with 2 short medial lobes, more medial lobe more robustly developed, bearing a stout, relatively long seta apically, other lobe short, apical seta shorter, large lateral lobe as wide as epandrial arm, slightly tapered ventrally, ventral margin rounded, in lateral view quadrate on basal half, thereafter apically thumb-like, broadly rounded apically; postsurstylus symmetrical, in lateral view (Fig. 7), elongate, moderately narrow, lateral margins sinuous, lacking a rod-like, extended process; subepandrial plate in ventral view moderately and widely W-shaped, lateral arms curved medially, acutely pointed; aedeagus in lateral view (Fig. 7) somewhat quadrate on basal half, apical portion narrower, thumb-like, apex rounded, in ventral view (Fig. 6) elongate, base wide, apical third abruptly narrowed, narrowly digitiform; phallapodeme in lateral view (Fig. 7) broadly lunate, extended keel wide but short, in ventral view (Fig. 6) elongate, slender, with basal crossbar Y-shaped and subapical short crossbars; pre- and postgonite fused, in lateral view (Fig. 7) a curved, rod-like process, bearing 2 setulae apically; hypandrium in ventral view (Fig. 6) broadly and robustly U-shaped, especially thickened anteriorly, anterior margin bluntly rounded, lateral margin with angulate extension, thereafter posteriorly narrowed, almost parallel sided, posterior margin truncate, in lateral view (Fig. 7) deeply pocket-like, anterior margin pointed, posterior margin extended as a tapered, narrow process toward phallapodeme. REVIEW OF MIMAPSILOPA FROM BRAZIL Zootaxa 3926 (4) © 2015 Magnolia Press · 503 Type material. The holotype male is labeled BRAZIL. Paraná: Matinhos, Rio da Onça[,] 25°47.1'S, 48°31.6'W; 3 m), 27 Jan 2010[,] D. & W. N. Mathis/Holotype ♂ Mimapsilopa acta Mathis, Costa, &Marinoni DZUP [red]. The holotype is double mounted (minuten in a block of plastic elastomer), is in excellent condition, and is deposited in DZUP. Seven paratypes (7♂; DZUP, USNM) bear the same label data as the holotype. Etymology. The species epithet, acta, is of Greek derivation, meaning seashore or coast, and refers to the coastal habitat of this species in southern Brazil. Type locality. BRAZIL. Paraná: Matinhos (Rio da Onça; 25°47.1'S, 48°31.6'W; 3 m). Distribution (Fig. 52). Neotropical: Brazil (Paraná). Remarks. This species is similar and is apparently closely related to M. cressoni Lizarralde de Grosso and M. bacoa Mathis and Zatwarnicki, but it is distinguished from either of these two species by structures of the male terminalia, especially the presurstylus, which is tapered apically and does not have a lateral incision. FIGURES 1–3. Mimapsilopa acta n.sp. (1) Head, anterior view; (2) Same, lateral view; (3) Same, oblique view. Scale bar = 0.2 mm. 504 · Zootaxa 3926 (4) © 2015 Magnolia Press MATHIS ET AL. FIGURES 4–7. Mimapsilopa acta n.sp. (4). Epandrium, cerci, presurstylus, posterior view. (5). Same, lateral view. (6). Aedeagus, hypandrium, phallapodeme, postsurstylus, pregonite, subepandrial plate, ventral view. (7). Same, lateral view. Scale bar = 0.1 mm. Mimapsilopa iguassu Mathis, Costa & Marinoni n.sp. Figs. 8–15, 49, 52. Diagnosis. This species is distinguished from congeners by the following combination of characters: Generally very dark brown, shiny; small to moderately small shore flies, body length 1.40–2.30 mm. Head (Figs. 8–10): Frons microtomentose brown on black background. Basal flagellomere mostly blackish, especially dorsoapically, only basoventromost portion yellowish, gradually tapered to rounded apex; arista with 7–10 dorsal rays. Face REVIEW OF MIMAPSILOPA FROM BRAZIL Zootaxa 3926 (4) © 2015 Magnolia Press · 505 mostly shiny bluish black to black, conspicuously microsculptured with shallow, mostly vertical and transverse striae. Eye ratio: 0.59–74; gena-to-eye ratio: 0.11–0.14. Thorax: Mesonotum sparsely microtomentose with brown microtomentum on shiny black background, appearing subshiny; anepisternum mostly shiny, only extreme margins microtomentose. Wing (Fig. 49) mostly infuscate, anterior margin brown, becoming progressively lighter colored posteriorly, posterior margin largely hyaline; length 1.50–2.20 mm; costal ratio 0.57–0.69; M vein ratio 0.73–0.91. Knob of halter white, stem yellowish. Foreleg, including forebasitarsomere and femora, and tibiae of mid- and hindlegs dark brown to black; extreme apex of mid- and hindtibiae and mid- and hindtarsi yellow except for darkened apical tarsomere. Abdomen: Tergites sparsely microtomentose, mostly shiny; tergites 4, 5 about equal in length, both longer than tergite 3; tergite 5 bare, shiny. Male terminalia (Figs. 11–15): Epandrium in posterior view (Fig. 11) setulose with short, moderately stout setulae, shape as an inverted, thick-walled U, widest at dorsolateral corners, conspicuously thinner at dorsal portion above cercal cavity, in lateral view (Fig. 12) height double width, FIGURES 8–10. Mimapsilopa iguassu n.sp. (8) Head, anterior view; (9) Same, lateral view; (10) Same, oblique view. Scale bar = 0.2 mm. 506 · Zootaxa 3926 (4) © 2015 Magnolia Press MATHIS ET AL. FIGURES 11–15. Mimapsilopa iguassu n.sp. (11) Epandrium, cerci, presurstylus, posterior view. (12) Same, lateral view. (13) Postsurstyli and postsurstylar process. (14) Aedeagus, hypandrium, phallapodeme, pregonite, subepandrial plate, ventral view. (15) Same, postsurstylus, lateral view. Scale bar = 0.1 mm. REVIEW OF MIMAPSILOPA FROM BRAZIL Zootaxa 3926 (4) © 2015 Magnolia Press · 507 anterior margin sinuous and with sub-basal short projection, posterior margin nearly straight; cercus in posterior view (Fig. 11) elongate, shallowly lunate but not sharply pointed at ventral and dorsal apices, in lateral view (Fig. 12) semi-hemispherical; presurstylus symmetrical, robust, in posterior view (Fig. 11) with one short medial lobe bearing an apical, stout setulae, thereafter ventrally robustly developed, ventral margin rounded, slightly tapered in lateral view (Fig. 12) quadrate on basal half, thereafter apically with short, thumb-like, broadly rounded apical process; postsurstylus symmetrical, in lateral view (Fig. 15), elongate, moderately narrow, sinuous, bearing short, fringe-like setulae on posterior margin, in ventral view (Fig. 13) pointed medially, angulate subapically, apex truncate, lacking a rod-like, extended process; subepandrial plate in ventral view moderately and widely U-shaped, lateral arms recurved, tapered toward apex, in lateral view (Fig. 15) as a parallelogram; aedeagus in lateral view (Fig. 15) somewhat rectangular on basal half, apical portion narrower, digitiform, apex narrowly rounded, in ventral view (Fig. 14) elongate, base ovate, apical third abruptly narrowed, narrowly digitiform; phallapodeme in lateral view (Fig. 15) broadly helmut-like, extended keel wide and moderately high, in ventral view (Fig. 14) elongate, slender, T-shaped; pregonite fused, in lateral view (Fig. 15) a curved, rod-like process, bearing 2 setulae apically; hypandrium in ventral view (Fig. 14) broadly and robustly semi-circular, anterior margin broadly rounded, laterobasal margin with angulate extension, thereafter posteriorly narrowed, in lateral view (Fig. 15) deeply pocket-like but with deep emargination, basal portion curved, bar-like, anterior portion triangular. Type material. The holotype male is labeled BRAZIL. Paraná: Parque Iguaçu[,] 25°33.4'S, 49°13.6'W; 880 m), 22Jan 2010[,] D. & W. N. Mathis/Holotype ♂ Mimapsilopa iguassu Mathis, Costa, & Marinoni DZUP [red]. The holotype is double mounted (minuten in a block of plastic elastomer), is in excellent condition, and is deposited in DZUP. Twenty-three paratypes (12♂, 11♀; DZUP, USNM) bear the same locality data as the holotype but with dates from 22 Jan–14 Apr 2010. Type locality. BRAZIL. Paraná: Parque Iguaçu (25°33.4'S, 49°13.6'W; 880 m). Distribution (Fig. 52). Neotropical: Brazil (Paraná). Etymology. The species epithet, iguassu, refers to the type locality, Parque Iguaçu, in the Municipality of Curitiba, Brazil, and is a noun in apposition. Remarks. Externally this species is similar to M. acta but can be distinguished from it and other congeners, especially those of the cressoni group, by the coloration and shape of the basal flagellomere, the pattern of infuscation of the wing, and by structures of the male terminalia. Mimapsilopa onssa Mathis, Costa & Marinoni n.sp. Figs. 16–22, 48, 52. Diagnosis. This species is distinguished from congeners by the following combination of characters: Generally very dark brown, shiny. Moderately small to medium-sized shore flies, body length 2.00–3.50 mm. Head (Figs. 16–18): Frons black, very sparsely microtomentose, subshiny to shiny; 2 proclinate fronto-orbital setae, anterior seta 3–4X length of posterior seta, posterior seta immediately laterad of large, reclinate fronto-orbital seta. Basal flagellomere brownish black, some specimens with faint orange coloration basoventrally, length nearly twice height, apex rounded; arista with 9–11 dorsal rays. Face black, polished, shiny, with very shallow, faint, vertical striae. Maxillary palpus black. Eye ratio: 0.61–84; gena-to-eye ratio: 0.11–0.14. Thorax: Mesonotum microsculptured; anepisternum dark brown, shiny. Wing (Fig. 48) with anterior margin infuscate, becoming less so to hyaline posteriorly, darkened portion in cell r with basal 1/4 noticeably paler than dark brown apical 3/4; wing 1 length 1.80–2.50 mm; costal ratio 0.63–0.67; M vein ratio 0.61–0.67. Knob of halter white, stem yellowish. Femora and tibiae black (brownish black in specimens taken from alcohol); foretarsus brownish yellow ventrally; mid- and hindtarsi with basal 2–3 tarsomeres yellow to brownish yellow, apical 2 tarsomeres blackish brown. Abdomen: Tergites sparsely microtomentose; tergites 4, 5 about equal in length, both longer than tergite 3; tergite 5 bare, shiny. Male terminalia (Figs. 19–22): Epandrium in posterior view (Fig. 19) moderately setulose, shape as an inverted, thin to thick-walled U, narrowed dorsally between dorsal margin of cercal cavity and anterodorsal margin, lateral arms about equally wide, ventral margin shallowly emarginate; in lateral view (Fig. 20) with height about twice width, widest subventrally, dorsal 2/3 tapered, anterior margin with convexity on ventral half, posterior margin more or less evenly rounded; cercus in posterior view (Fig. 19) irregularly lunate, tapered toward apical angle, apical curved medially, in lateral view (Fig. 20) irregularly semicircular, curvature more abrupt dorsally; presurstylus symmetrical, in posterior view (Fig. 19) as a bilobed structure, with median lobe much shorter, rod- 508 · Zootaxa 3926 (4) © 2015 Magnolia Press MATHIS ET AL.