SPIXIANA BeardiusfoundinENP.Uponcomparingmaterialofdescribedspecies,twoparatypelarvaeofß.parcus were found to represent a different, presently unassociated form. The first description for the female ofB.aciculatus,collectedinMexico,couldbeaddedtothisreviewthroughcourtesyofDrsA.Contreras Ramos and T. Andersen. Methods Adults, pupae, andfourthinstarlarvaeforrearingwerecollectedwhilesamplingforpupalexuviaeat the water surface with a dipping pot and 125 ^Hm-opening sieve, preserved in 95% ethanol, and mounted in Euparal. All material listed was collected by the senior author and is kept in the ENP museum or in his personal collection unless indicated otherwise. Morphological terminology and abbreviations follow S^ther (1980) and Langton (1995), except 'taeniae' and 'taeniate' are used as suggested by Langton (1994) to describe flattened L, D, De, and anal lobe fringe setae of pupae. Measurements follow Soponis (1977), where applicable. Inferior and superior volsella lengths were measured from their junction to the respective apices. Lateral spines on tergite VIII were measured from the posterior margin of their base. Mentum width is the distancebetween the outer margins of thefifthlaterals,notthewidthatthebase.Postmentumlengthisthedistancefromtheapexofthepale median tooth to the post-occipital margin. Measurements are reported as a ränge, foUowed by the meanwhen sample sizen>3, foUowedbyninparentheses. All measurements arein ^munless stated otherwise. Abbreviations of life stages: L = larva, P = pupa, ex = exuviae. Abbreviationsofnamesandinstitutions: ERCC=EvergladesRegionCollectionCenter, Everglades National Park; JHE = J. H. Epler; REJ = R. E. Jacobsen; ZMBN = Zoological Museum, University of Bergen, Norway; ZSM = Zoologische Staatssammlung, Munich, Germany. Beardius Reiss & Sublette 1985, emended Beardius Reiss & Sublette, 1985: 179; Andersen & Saether 1996: 34. Type-species: Beardiusparcus Reiss & Sublette, 1985: 183, by original designation. Description The description by Reiss & Sublette (1985) and emendation of Andersen & Ssther (1996) can be further emended as foUows: Acrostichal setae ofnormal length, reduced, orlacking. Maleinferiorvolsellauprightortntumed; median volsella acicular, narrowly conical to truncate-conical, cylindrical-coronate, triangulär, or tongue-shaped. Female gonocoxite IX small, bare or with 1 seta. Seminal capsule with or without microtrichia; spermathecal duct wide, straight, bare or with thorn-like special secretory cells along proximal half. Thoracichörnofpupa with6strong, smoothbranches, orwithanteriorbrauchnotablylargerand besetwithfine spinules. Tergites II-VIeachwithconspicuous anteriorband ofstrong spinules contin- uous with median field of finer shagreen, or T II with uniform strong shagreen and T III-VI with anteriorpointsonlyslightlylargerthanposteriorpoints.ConjunctivesglabrousexceptIV/Vwhichhas acontinuouswidebandofanteriorlydirectedstrongspinules,andl/IIwhichmayhaveamedianpatch offine spinules. Paratergite Vwith fine spinules insmall posterior patchor asband extendinghalfto nearly fülllengthofsegment. Posterolateral corner ofsegmentVIII withwide, short, single or double spurusuallybearingbasalhair-finespinules [bareonsome B. sp. B]; anteriorlycontiguoustospurare small to large, straight to posteriorly-curved spines or Clusters of spinules. Segment V with 3-4 pairs of taeniate L setae. Anal lobe with few (<25) uniserial fringe taeniae. Larval head with labral sclerite 2 present; clypeus separate or fused into frontociypeal apotome. Antenna with 5, 6, or 7 segments; basal segment somewhat shorter to slightly longer than flagellum. Mola of mandible with 0-2 spines. Mentum with first lateral tooth notched; ventromental plates separated by 1-2 times width of median tooth of mentum, each about % to füll width of mentum. 130 Key to adult males of Beardius Reiss & Sublette The key in Andersen & S^ther (1996) is modified with the foUowing changes to couplets 1 and 2: 1. Superior volsella pediform, withbasal and median microtrichia; inferior volsella with thick stem and spine-like, apically dissected sensilla chaetica. HR > 0.89 parcus group, 2. - Superiorvolselladigitiform,atmostwithafewbasalmicrotrichia;inferiorvolsellaslender,slightly clavate, without spine-like sensilla chaetica: HR < 0.84 truncatus group, 3. 2. Medianvolsella spine-like; withone seta, otherwisebare. Scutal tubercle prominent. (Andersen & Sffither 1996: fig. 3) aciculatus Andersen & Saether - Medianvolsellashort,triangulärtocylindrical-coronate,withapicalClusterofmicrotrichiaorsetae. Scutal tubercle absent or vestigial 2a. 2a. Medianvolsellatriangulär,withabout10-15longapicalmicrotrichiaorsetae;inferiorvolsellawith thick stem and lacking knob-like apex, sensilla chaetica slender. Wing vein R without setae. Acrostichals absent. (Reiss & Sublette 1985: figs 1-14) parcus Reiss & Sublette - Median volsella short, cylindrical to coronate, with 4-6 apical setae; inferior volsella with slender stem and enlarged knob-like apex, sensilla chaetica stout. Vein R with 4-8 setae. 3-6 acrostichal setae. (Figs la-c, e) reissi, spec. nov. Tentative key to adult females of Beardius Reiss & Sublette (ß. breviculus, lingulatus, triangulatus, truncatus extrapolated from males, not yet collected) 1. Scutum without a tubercle or broad anterior hump 2. - Scutum with a tubercle or broad anterior hump 3. mm 2. Acrostichals present. Palpomere 5 length <120 reissi, spec. nov. - Acrostichals absent. Palpomere 5 length >120 mm triangulatus Andersen & Saether 3. Acrostichals present truncatus Reiss & Sublette - Acrostichals absent 4. 4. Scutum with small tubercle with strong anterior and posterior sloping so that it clearly projects above surrounding surfaces 5. - Scutum with broad anterior hump that projects clearly above only the posterior scutum surface 6. 5. Clypeus with 16-24 setae, abdomen and legs with color patterns ...aciculatus Andersen & Saether - Clypeus with less than 16 setae, abdomen and legs pale lingulatus Andersen & Saether 6. Wing with setae on R breviculus Reiss & Sublette - Wing without setae on R parcus Reiss & Sublette Key to pupae of Beardius Reiss & Sublette (ß. aciculatus, breviculus, lingulatus, triangulatus unknown) 1. Tergite II with shagreen points distinctly stronger in anterior transverse band; paratergite Vwith finespinulesextendingoveratleasthalfsegmentlength;TIII-VIeachwith4pairsofDsetae; (Fig. 2d; Reiss & Sublette 1985: fig. 15) 2. - Tergite II shagreen points strong and uniform throughout; paratergite V with fine spinules con- fined to small posterior patch; T III-VI each with 5 pairs of D setae; (Fig. 3g) 3. 2. Abdominal segment VIII with 1-8 strong spines along lateral margin anterior to spur (Fig. 2c). Frontal apotome length/ width about 0.9, apex acute (Fig. 2a) reissi, spec. nov. 131 AbdominalsegmentVIIIwithatmostonlytinyspinulesalonglateralmarginanteriortospur(Reiss & Sublette 1985: fig. 19). Frontal apotome length/ width about 0.5-0.6 parcus Reiss & Sublette Abdominal segment V with 3 taeniate L setae. Thoracic cuticle with unpigmented area along the oblique hinge line near the median suture (Fig. 3d). Conjunctive I/II bare; pigmentation within shagreen on tergite II diffusely spread between points (Fig. 3f); hook row of30-44 hooklets sp. B AbdominalsegmentVwith4taeniateLsetae. Thoraciccuticlepigmentedalongtheobliquehinge linenearthemediansuture (Fig. 3c). ConjunctiveI/IIwithmedianpatchoffinespinules(Fig. 3g); pigmentation within shagreen on tergite II confined to discrete blotches at point bases (Fig. 3e); hook row of 46-73 hooklets truncatus Reiss & Sublette Key to larvae of Beardius Reiss & Sublette (B. aciculatus, breviculus, lingulatus, triangulatus unknown) 1. Antenna with 7 segments (Fig. 5b), but segments 2-4 may be only partially separated 2. - Antenna with 5 or 6 segments 3. 2. Clypeus distinct from frontal apotome (Fig. 6a). Ventromental plates separatedby aboutwidthof palemediantoothofmentum.Procercuswith8analsetae.AR0.87-1.05.(Reiss&Sublette1985:figs 21-25) parcus Reiss & Sublette - Clypeus fused intofrontoclypeal apotome (Fig. 6d). Ventromentalplates separatedbyabouttwice widthofpalemediantoothofmentum (Fig. 5a). Procercuswith7analsetae. AR0.70-0.79 (Fig. 5b) C sp. 3. Antennawith5 segments (Epler 1992, 1995: 7.27). Surface offrontoclypeal apotome granulärfrom anterior margin to S4 setae (Fig. 6c) truncatus Reiss & Sublette - Antennawith6segments(Fig.2e).Surfaceoffrontoclypealapotomegranulärfromanteriormargin to about % distance to S4 setae (Fig. 6b) reissi, spec. nov. Beardius reissi Jacobsen, spec. nov. (Figs 1, 2, 6b) A Beardius sp. Epler, 1992: 7.27; 1995: 7.27. Types. Holotype: 3(slide-mountedinEuparal);USA:Florida,DadeCo.,EvergladesNationalPark,marlprairie alongroadtoRoyalPalm,onwatersurface,20.IX.1999,R.E.Jacobsen;depositedinZoologischeStaatsammlung, Munich,Germany.-Paratypes: Florida,DadeCo., EvergladesNatlPark:marlprairiealongFL9336 1.5mi.W. ofTaylorSlough: 19, IcJ,bothwithPex+Lex, rearedfromPandLcollected17.IX.1999,emerged 18.Xand27.X; 2$+Pex+Lex,rearedfromPcollected6.IX.1999,emerged8.IX;2?$,6.IX.1999, 1Lex,6.X.1998;aspreviousexcept 1 mi. W. ofTaylor Slough: 266, 1 Lex, 28.VIII.1999; 1 L, 1 Lex, 1 female P+Lex, 1 Pex+Lex, 29.Vin.1999; 16, m IcJ+Pex+Lex reared from P, collected 6.IX.1999, emerged 8.IX.1999; as previous except400 E. ofRock Reef Pass: 1 Pex,7.X.1998,3L,4.VI.1999, 1L, 11.VI.1999, 16, 13.VI.1999, 10Pex,20.VI.1999;marlprairiealongL-31W canal 400 m E. ofTaylorSlough: lc?+Pex+Lex, reared from L collected 17.IX.1999, emerged 27.IX; asprevious except 1 mi. N. ofTaylorSlough: 6L, 7.X.1998, M. C. Bruno&R.Jacobsen;LongPineKey, Solutionholealong WildernessRoad:10Pex,16.+20.VII.1998,M.CBruno&R.Jacobsen,16,30.1X.1999;Chekika,madprairiealong SW 237 Avenue 1 mi. S. of Chekika entrance: 16, 27.IX.1999. Hardee Co., Big Slough at SR 72: 3 L, dipnet, 24.Vm.1999, J. Lancaster & D. Durbin. Paratypes deposited at ZSM, ZMBN, ERCC, and in collsJHE, REJ. Diagnosis. Adults of B. reissi, spec. nov. are distinguished from other congeners by the presence of acrostichalsetaeandthelackofamediantubercleoranteriorhumponthescutum.Inmales,theshort, cylindrical-coronate median volsella with 4-6 apical setae, and the club-like inferior volsella bearing stout, apicallydissectedsensillachaeticaare alsodistinctive. Thepupaischaracterizedbytheanterior transversebandofstrongerpointsontergiteII, thepresenceofspines alongthelateralmarginofVIII, andtheapicallyacutefrontalapotomewithlength/widthabout0.9.The6-segmentedantennaandthe 132 Fig.1. Beardiusreissi,spec.nov.;adults(maleexceptd.). a.Hypopygium,left:dorsal,right:ventral, b.Superior volsella. c. Inferior volsella, two views. d. Female genitalia, ventral, e. Thorax. frontoclypeal apotome with anterior granulation abruptly ending well anterior to the S4 setae are distinctive features of B. reissi larvae. Etymology. NamedinhonorofFriedrichReissforhiscontributionstochironomidology,includingerectingthe genus Beardius withJames Sublette. Description Adult male (n=8 unless stated otherwise). Total length 2.28-2.67, 2.50 mm. Wing length 1.11-1.36, 1.19 mm. Total length/wing length 1.91- 2.20, 2.11. Wing length/profemur length 1.64-1.99, 1.77. Alcohol-preserved specimens stramineous exceptvittae,mediananepisternumIIandpostnotumbrown;pedicelandlegslightbrownwithapices of tibiae darker. Head. AR0.81-0.96, 0.88; terminal flagellomere323-404, 357long. Temporal setae8-12, 10inSingle row. Clypeus with 6-9, 7 setae. Interocular distance 115-132, 121. Tentorium 98-114, 109 long. Pal- pomere lengths 19-25, 22; 24-31, 28; 59-70, 65; 60-77, 70; 73-113, 93; Pm3 with2-5, 3 lanceolate sensilla clavata. 133 Thorax (Fig. le). Scutal tubercleabsent. Antepronotalsetaeabsent; acrostichals3-6, 5, notreduced; dorsocentrals 4-6, 5 in Single row; prealars 1-2, 2; scutellars 2-4, 3 in Single row. Wing. VR 1.17-1.29, 1.21. Punctationvisible at200X. Squamabare. Brachiolumwith2 setae; R4-8, 5; Ri 0-2, 0; R4^5 1-2, 2. Legs.Tibialspurlengths:fore25-36,30;mid40-48,44;bind41-48,45.Tibialapexwidths:fore43-53, 49;mid44-50, 49; bind 49-55, 51. Mid ta^ with0-1, sensilla chaetica positioned 0.78-0.87lengthoftaj from base; sensilla chaetica absent on bind taj. Lengths and proportions of legs (n=7): fe ti tai taj tag ta4 Pl Fig. 2. Beardius reissi, spec. nov.; pupa andlarva. a-d. Pupa. a. Frontal apotome. b. Thoracichörn, schematic. c. Abdominal segment VIII lateral margin, dorsal, d. Tergites II-VI. e-h. Larva, e. Antenna. f. Mandible. g. Maxilla. h. Mentum. Abdomen. Number of setae on tergites I-VIII (n=3): 15-20, 12-19, 17-25, 18-25, 21-25, 19-25, 22-24, 11-18. Number of setae on sternites I-VIII (n=3): 0, 0, 2-3, 11-14, 18-21, 19-21, 21-24, 19-21. Genitalia (Fig. Id). Tergite IX with 18-24, 20 setae. Gonocoxite IX bare. Cercus 61-77, 68 long. Seminal capsule24-33, 29wide;37-45, 42longincluding9longneck; apparentlywithoutmicrotrichia. Spermathecal duct straight, without special secretory cells. Notum 132-146, 139 long. 135 Pupa (n=18, unless stated otherwise). mm Total length 3.02-3.80, 3.44 (15). Cephalothorax light brown; darker atbases of anteraiae, leg and wing sheaths, on wing margins, post-occipital area, and median suture of frontal apotome; abdominal tergites clear with brown shagreen points; paratergites V-VIII with progressively wider, darker longitudinal pigment bands; anal lobes brown near bases of taeniae. Cephalothorax. Antennal sheath usually with minute pearl row above pedicel. Frontal apotome apically acute (Fig. 2a), length/width about 0.9; frontal tubercle apically pointed, thorn-like, 12-23, 17 long, 10-18, 14Wide,withsubapicallyinsertedfrontalseta22-38,28long.Lateralantepronotumusually with vestigial seta and occasionally an additional setal alveolus. Thoracic hörn (Fig. 2b, schematic) apparentlywith6branches: astoutanteriorbranchwithsmall spinulesnearapex, amedianarmwith two slender, smooth branches, and a posterior arm with 3 slender, smooth branches; basal ring elliptical, 24-39, 31 long, with one tracheal branch 10-16, 13 wide. One precorneal seta, 28-47, 37 (13) long. Dorsocentral setaDcj stiff, spine-like, alveolus weakly sclerotized; De, andDcj slender, taeniate, alveoli strongly sclerotized; DC4 hair-like, alveolus unsclerotized; lengths: Dcj18-35, 26; Dcj 30-52, 42; DC322-48,34;DC48-14,11;distanceDcjtoDC25-23, 10;DC2toDcj114-143, 129;DcjtoDC44-10,5.Usually with 1 vestigial prealar seta or sensilla and 1-2 setal scars. Abdomen. Tergites I, VII, and VIII without shagreen; T II-VI (Fig. 2d) with anterior, transverse band of points distinctly stronger than posteriorly contiguous shagreen, the latter with increasing Segment number progressively separating into 2 lobes and reduced until only few points present on VI. ConjunctiveIV/Vwith3-4rowsofanteriorly-directedpointsor2-5-toothedpointrows, pointfield extends % width of segment. Faratergite V with extensive field offine points starting nearbase ofLj and extending almostto posteriormargin, all otherpTbare. Hookrow onII continuous, extending% widthofsegment, of27-59, 39hooklets. SternitesI, VII, andVIIIbare;SIIwithmedianfield and afew posterolateralfinepoints;IIIwithanteriorfield; IVwithlateralband;VandVIwithscattered anterior finepoints. Pedes spuriiA onFV. Pedes spurii B weaklydeveloped onII. SegmentVIIIlateralmargin (Fig. 2c)with2-7, 3strong, straightorposteriorly-curved spines, longestspine 19-36, 27;alsooccasion- allywithisolated or clustered small spinules. Anal spur Single ordouble, 8-17, 13 long, withhair-like spinulesatbase. Malegenitalsacextends60-99 (3)beyondanallobe.Setation:SegmentIwith2D, L, and 1 V seta; II with 2 D, 3 L, and 2 V setae; III-IV with 4 D, 3 L, 3 V setae; V with 4 D, 3 taeniateL, 3 V setae; VI with 4 D, 4 taeniate L, 3 V setae; VII with 2-4 (usually 2) D, 4 taeniate L, 3 V setae; VIII with0-1 D,4taeniateL, 1 Vseta.D3setaonIII-VIdistinctlytaeniate and longerthanothers. Anallobe with 13-22, 17 fringe taeniae. Larva (n=14-16, unless stated otherwise). Body length 3.36-5.10, 4.30 mm (9). Head capsule length 0.28-0.39, 0.34 mm. Head lightbrown to brown, body red-orange with dark green markings on Segments I-III and some light green markings on rV-V; specimens freshly removed from hoUowed twigs and grass culms have head, posterior Segmentsand analtubulesnoticeablyredderthanotherSegments; alcoholpreservedlarvaeareyellow with light brown to brown head capsules. Head dorsum (Fig. 6b). With labral sclerite 2 and frontoclypeal apotome, the latter distinctly granulär from anterior margin to approximately % distance to S4 setae. Antenna (Fig. 2e). 6-segmented. AR0.90-1.18, 0.98. Segmentlengths53-76, 63; 12-18, 14; 12-17, 14; 14-19, 16; 11-17, 14; 6-8, 7. Basal segment 16-27, 20wide; distance frombase toringorgan 12-17, 14, to basalsetalmark 13-18, 14, to distal mark46-65, 54. Blade55-79, 65 long, subequalto orslightlylonger thanflagellum;accessoryblade8-12, 10long.Lauterbornorganslarge, 12-14, 13long;segment3apical style 7-10, 9 long. Labrum. SI slender, tapering, only medially fimbriate, Sil only apically fimbriate. Labral lamella withuniformfringe. 2spinulae, and4longposteriorand5-6 shortanteriorchaetulae. Pectenepiphar- yngiscomposedof3separateplates,eachwith3-5apicalteeth,with8chaetulaelateralesand2apically notched chaetulaebasales. Premandible67-88, 80long,bifid, withbrush. Setapremandibularissimple. Mentum (Fig. 2h). Width 87-104, 97 (12). With a pointed to worn-rounded pale mediantooth and fivepairs ofdarklateral teeth. Firstlateral notched, fourth and fifthbasally fused. Ventromentalplate 62-86, 73 wide, with smooth anterior margin and approximately 20-30, 27 Striae. Ventromental plate width / mentum width 0.65-0.84, 0.76. Postmentum 148-193, 172 long, with pebbled surface texture between ventromental plates. Seta submenti with 1-3 short, splinter-like branches. Maxiila (Fig. 2g). With 3 scale-like lamellar and 2 fimbriate lacinial chaetae. 136 1 ' --r> 11'!!. ^_ i^ i 11J£3 '1 J .-^ PI W k y I ^ ^ I ^ i 1 1 >i ,"«" ' f 1 1 (1 i\11 'j Lj. Fig. 3. Beardhis tnmcatus Reiss & Sublette and ß. sp. B; pupae. a-b. Frontal apotome. c-d. Thorax, lateral. e-f.TergiteIIshagreenpigmentationpattern. g.TergitesI (posterior)toVI. a,c,e,g. ß. truncatus. b,d,f.ß. sp.B. Mandible (Fig. 2f). Length 123-152, 141. Outer margin often with lightly pebbled or wrinkled surface. Pecten mandibularis oi about 8-10 lamellae. Seta interna with 3 extensively branched arms, Iowest arm with 2 majorbranches. Mola with 1-2 small spines. Body. Anterior 3 segments with conspicuous setae, posterior segments without notable setae. Procercus 23-36, 31 (10) high, 30-38, 34 (10) wide, with 8 anal setae up to 397-490, 438 (11) long. Supraanal seta length 294-416, 338 (11). Supraanal/anal seta ratio 0.68-0.91, 0.77. Posterior parapod about 125-165, 145 (6) long. Anal tubules conical-digitiform; ventral 72-94, 83 long, 44-62, 52 (5) wide; dorsal 65-86, 76 long, 34-46, 38 (5) wide. Comments. The3 larvaefromBigSlough, Hardee Co., differfrom reared specimensfrom Everglades National Park inhaving a longer, darker, more sclerotized head capsule and a higher antennal ratio. The greater size and sclerotization oflarvae from Big Slough maybe due to lower ambient tempera- tures and/or nutrient enrichment from agriculture at this site. 137 Beardius truncatus Reiss & Sublette, emended (Figs 3a,c,e,g, 6c) Beardius truncatus Reiss & Sublette, 1985: 188 (male); Epler 1992: 7.27, 1995: 7.27 (larva). Material examined. USA, Florida: ColumbiaCo., SantaFeRiveratUS441 atHighSprings, 1 maleP+Lex, IS, 1$ P, 13.Xn.l990, Environmental Sciences and Permitting personnel (in coli. JHE). Dade Co., Everglades Natl Park, Solutionhole alongL-31Wcanal 2mi. N. ofTaylorSlough, 2 Pex, 21.XI.1999; Solutionhole inLongPine Key alongWildemessRd, 4Pex, 16.Vn.l998, M. C. Bruno &R.Jacobsen; 1 mileW. ofTaylorSloughalongFL 9336,3Pex,7.X.1998,7Pex,5.II.1999,D.Levitis&R.Jacobsen;TaylorSlough100mdownstreamofL-31Wcanal, 1 Pex, 12.V.1999. Description Pupa (n=17-19, unless stated otherwise) mm Totallength3.19-4.20, 3.62 (10). Cephalothoraxlightbrowntogray, darkeratbasesoflegand wing sheaths, on v^^ing margins, post-occipital area, and median suture of frontal apotome between cephalic tubercles; thoracic cuticle along oblique hinge line (Langton 1995: 184) with light brow^n pigmentation near median suture (Fig. 3c); tergites with pale yellovs^, brown or gray pigmentation; pigmentationstrongestonTI, progressivelydiminishingonsuccessivesegments;pigmentationwithin shagreen fields confined to points and discrete patches atbases ofpoints (Fig. 3e); paratergitesV-VIII with increasingly wider, darker longitudinal bands of pigment. Cephalothorax. Antennalsheathwithorwithoutminutepearlrowabovepedicel. Frontaltubercle arisingfrom a lowprominence (Fig. 3a), apicallypointed, thorn-like, 17-28, 22 (10)long, 14-20, 17(10) Wide, with subapical-anteriorly inserted frontal seta 30-40, 35 (15) long. Antepronotum occasionally with alveolus or vestigial seta. Thoracichörn not clearly observable in available mounts, with a stout anteriorbranchwithspinulesonatleastthedistalhalf;andatleast3other,smoothbranches;basalring elliptical, 34-56,42long, with 1 trachealbranch12-20, 15wide. 1 taeniateprecorneal, 36-44,41 (4)long. Dorsocentral seta Dci stiff, hairlike, alveolus well sclerotized; Dcj and DC4 taeniate, alveoli strongly sclerotized; Dcjhairlike, alveolus lightly sclerotized; lengthsDe,28-43, 35; DC2 31-67, 48; DC3 48-65, 56 (16) ; DC4 18-31, 24; distance Dcj to DC2 6-17, 12; Dcj to De, 150-248, 207; Dcj to DC4 9-20, 13. With or without prealar setal scars. Abdomen. Tergites I, VII, and VIII without shagreen; T II (Fig. 3g) shagreen widest in anterior third, narrowing abruptly behind anterior muscle marks and widening over posterior half; central points strong, generally uniform in size, diminishing only at periphery; III to V with progressively smaller, more posteriorly-bilobed fields, with anterior points notably larger than posterior points; VI has anteromedian field with short posterior lobes of smaller points. Conjunctive I/II with a small medianpatchofveryfinespinules;IV/Vwith4-5rowsofanteriorly-directedpointsorpairsofpoints, point field extends about % width of segment. Paratergite V with posterior patch offine spinules, VI usually with a few fine spinules near base of L4. Hook row on II continuous, extending % width of segment, of46-73, 59hooklets. Sternites1, IV-VIIIbare;IIandIIIwithposteromedianfield ofscattered fine points. Pedes spurii A on IV. Pedes spurii B present on II. Segment VIII lateral margin with 0-8, 3straightorposteriorly-curvedspines,longestspine17-46,25long.AnalspurusuallySingle, occasion- ally double, 7-14, 10 long, with hair-like spinules at base. Male genital sac extends 94-109, 104 (4) beyond anal lobe. Setation: Segment I with2 D, L, 1 V seta; II with2 D, 3 L, 3 V setae; IIl-FV with5 D, 3 L, 3V setae; V-Vl with 5 D, 4 taeniateL, 3V setae; VII with3D, 4 taeniate L, 3 V setae; VIII with D, 4 taeniate L, 1 V seta. D4 seta on IIl-VI distinctly taeniate and longer than others. Anal lobe with 12-18, 15 fringe taeniae. Beardius sp. B (Figs 3b,d,f) Materialexamined. USA: Florida,DadeCo., EvergladesNatlPark,TaylorSlough, alligatorhole 1 mi. S. ofFL 9336, 2 Pex, 12.11.1999; Typha stand 1 mi. S. ofFL 9336, 1 Pex, 14.1.1999, 1 Pex 12.11.1999; Cladium stand 1 miS. ofFL9336, 1 Pex, 12.11.1999, allD. Levitis&R.Jacobsen;200mW. ofFL9336bridge, 1 Pex, 6.X.1998;200mE. ofFL9336bridge, 1 Pex, 5.11.1999; upstream ofFL 9336 bridge, 3 Pex, 5.II.1999. 138