WiLLMOTT: A new subspecies of Cithaerias pyropina TROP. LEPiD. RES., 25(1): 1-4, 2015 1 A NEw SUBSPECIES OF Cithaerias pyropina (SALVIN & GODMAN, 1868) FROM THE CORDILLERA DE CUTUCú IN SOUTHEASTERN ECUADOR (NYMPHALIDAE: SATYRINAE) Keith R. willmott McGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of Florida, Gainesville, Florida, USA; [email protected] Abstract – The new subspecies Cithaerias pyropina julia Willmott, n. ssp., is described from the Cordillera de Cutucú, an isolated sedimentary mountain range in southeastern Ecuador. This is the first new insect taxon to be described from this mountain range, to which it currently appears to be endemic. The new subspecies is distinguished from other subspecies in Peru and Bolivia by its extensive dark blue iridescence and lack of pinkish markings on the dorsal hindwing. Resumen – Se describe la nueva subespecie Cithaerias pyropina julia Willmott, n. ssp. de la Cordillera del Cutucú, una cordillera aislada de origen sedimentario en el este del Ecuador. Este representa el primer taxón nuevo descrito de esta Cordillera, donde aparentemente es endémico. La nueva subespecie se distingue de otras subespecies en Perú y Bolivia por su color de azul iridescente y la ausencia de marcas rosadas en el ala posterior en la cara dorsal. Key words: Bolivia, Cithaerias pyropina julia Willmott n. ssp., Cithaerias pyropina pyropina, Cithaerias pyropina songoana, cloud forest, Andes, Cordillera de Kutukú INTRODUCTION entomologist Martin Cooper made a collection of insects in the late 1970s and early 1980s along a trail that crossed the The Cordillera de Cutucú (also spelled Kutukú and Kutucú) mountain range due east of Macas to the Río Mangosisca (M. is an isolated mountain range in southern Ecuador, rising from Cooper, pers. comm.). Few butterflies were collected at higher around 300 m in the lowlands to 2500 m. With the Río Upano to elevations, unfortunately, and only the ithomiine Hylayris the west and the Río Santiago to the south, the highest point of antea achuar Vitale & Bollino, 2000, which also occurs in connection to the main Andean cordillera is in the northern part the adjacent Andes above Macas, provided any indication of of the range at approximately 1150 m. The Cordillera forms faunistic affinities. part of a series of mountain ranges lying at the eastern edge of As part of a long-term inventory of the butterflies of the Andes, including the Cordillera del Cóndor to the south and Ecuador (www.butterfliesofecuador.com), we made a short the Cordillera Galeras to the north, whose unusual sedimentary expedition into the Cordillera de Cutucú to sample butterflies at geology has resulted in these peaks showing the distinctive middle elevations in 2003. The expedition resulted in a number table-top profile of the Guianan highland ‘tepuis’. of interesting cloud forest butterfly taxa (e.g., Pachacutia The Cordillera del Cóndor has been the target of various baroni Willmott & Lamas, 2007), including a new taxon in biological expeditions (e.g., Schulenberg & Awbrey, 1997; the small genus of transparent satyrine butterflies Cithaerias Neill, 2007; Guayasamín & Bonaccorso, 2011), and, although Hübner, [1819]. the butterfly fauna appears to be unrelated to that of the Guianan Cithaerias is confined to the Neotropics and contains fewer tepuis (e.g., Costa et al., 2013), it is still known to harbor a than 10 species, most of which are found in lowland forests, number of endemic taxa (e.g. Lamas, 1997; Radford & Willmott, especially in the Amazon (Lamas, 2004; Penz et al., 2014). The 2013), although most remain to be described. The Cordillera immature stages feed on Araceae (e.g., Murillo, 2009; Janzen & de Cutucú, however, remains extremely poorly studied. An Hallwachs, 2015), common plants in the understorey of humid ornithological expedition in 1984 surveyed four sites on the forest. Often noticed by non-lepidopterists because of their western slopes from 1000-2300 m, accessing the mountain abundance, low flight and conspicuous hindwing coloration, the range along the old trail from the town of Logroño in the west taxonomy of Cithaerias is nevertheless still poorly understood to Yaupi in the east. The avian fauna recorded proved to be very (e.g., Penz et al., 2014). I here describe a new subspecies of similar to sites in the Cordillera del Cóndor and adjacent Andes the east Andean Cithaerias pyropina (Salvin & Godman, (Robbins et al., 1987), but several anuran amphibians collected 1868), perhaps the only true cloud forest species in the genus, during that expedition proved to be undescribed and apparently to contribute to improving our knowledge partly of Cithaerias endemic to the mountain range (Duellman & Lynch, 1988), taxonomy, and partly of the fauna of the Cordillera de Cutucú. opening up the possibility of endemic taxa in more sedentary organisms. Aside from this expedition, Brito & Pozo-Zamora METHODS (2013) recently reported a new species of frog collected during an expedition in 2012 along the same trail. Otherwise, I have Morphology was studied using standard techniques, with not been able to find any other publications about the fauna of adult abdomens being soaked in hot 10% KOH for 10-15 the Cordillera at elevations above 1500 m. minutes, dissected and subsequently stored in glycerine. Body Similarly to other organisms, therefore, the butterflies of the morphology and dissections were studied using a binocular Cordillera de Cutucú also remain very poorly known. British microscope at 50x magnification. The terminology for male 2 TROP. LEPiD. RES., 25(1): 1-4, 2015 WiLLMOTT: A new subspecies of Cithaerias pyropina genitalic and abdominal structures follows Scoble (1992), and and the dark iridescent surrounding patch of scales is a paler, nomenclature for venation follows Comstock & Needham sapphire blue rather than dark purplish blue. In contrast, the (1918). white submarginal spot in DHW cell M3-M2 is larger than in the Distributional data and field observations for Cithaerias nominate subspecies, being similar in size to the corresponding were also gathered by myself and colleagues during more than spot in cell Cu1-M3. Overall, the hindwing tornal colored 700 days of field work in Ecuador between 1991 and 2014, patch appears dark blue in flight, without any evidence of pink representing 600 sites in 20 provinces, ranging from sea level coloration. to 4000 m on both Andean slopes. Access to the Cordillera de Cutucú was from the village of Tayuza, just south of Logroño. Taxonomy: Cithaerias pyropina is currently treated as Butterfly sampling was conducted at three sites along a trail containing two subspecies (Lamas, 2004). The nominate leading from the village of Yakunk (02°44.432’ S, 78°12.242’ subspecies was described by Salvin & Godman (1868) from W) up into the western slopes of the Cordillera, including Peru (Type locality [TL]: Peru: lower Huallaga; Pozuzo), and flat primary forest at 1000 m (02°45.112’ S, 78°10.913’ W), the names Callitaera pyropina roquensis Bryk, 1953 (TL: Peru: ridgetop primary forest at 1340-1400 m (the type locality), and Roque) and Callitaera pyropina var. rosacea Langer, 1944 ridgetop secondary forest and pasture at 1570 m (02°45.815’ S, (TL: Peru: upper Huallaga) are currently treated as synonyms 78°08.662’ W). (Lamas, 2004). Cithaerias pyropina songoana (Langer, 1944), Recent taxonomic papers on the genus (Constantino, from Bolivia (TL: Río Songo), differs from the nominate 1995; Penz et al., 2014) were examined for relevant taxa subspecies in having a pink flush extending across the dark and information. Original descriptions of all names listed as blue areas in the DHW tornus, thus somewhat resembling the subspecies or synonyms of C. pyropina by Lamas (2004) were lowland species Cithaerias pireta (Stoll, 1780). also examined, and information from those descriptions was The male genitalia of C. pyropina are rather distinctive in used to confirm the validity of the putative type specimens of all comparison with C. pireta and relatives (Constantino, 1995; of these names illustrated on www.butterfliesofamerica.com. Penz et al., 2014). The taxon described here is treated as a subspecies of C. pyropina based on several distinctive male RESULTS AND DISCUSSION genitalic characters shared with a dissected male of C. pyropina pyropina from Tingo María (Peru: Huánuco; FLMNH genitalia Cithaerias pyropina julia willmott, new subspecies dissection vial KW-14-84), and (where visible) in illustrations Figs. 1-3. of male genitalia of C. p. pyropina and C. p. songoana in Langer (1944). These include the flared anterior tip to the aedeagus in Description and diagnosis: This subspecies differs from dorsal view (Fig. 2C), multiple projections and cup-like inner the nominate subspecies (Fig. 1) by having smaller, pale valve surface at the distal tip of the valvae (Fig. 2A) and the submarginal and marginal spots in DHW cells 2A-Cu2, Cu2- short, rounded, studded subscaphium (Fig. 2B). Insufficient Cu1 and Cu1-M3, which appear dirty white rather than pinkish, specimens were examined to determine whether there were any Fig. 1: Cithaerias pyropina subspecies: left, ventral surface; middle, dorsal surface; right, dorsal surface over dark background. A. Cithaerias pyropina julia n. ssp., HT♂, Ecuador. B. C. pyropina songoana, ♂, Bolivia. C. C. pyropina pyropina, ♂, Peru. D. C. pyropina pyropina, ♀, Peru. WiLLMOTT: A new subspecies of Cithaerias pyropina TROP. LEPiD. RES., 25(1): 1-4, 2015 3 fallen branches and appearing very inconspicuous with their muted hindwing coloration. No individuals were observed at a flat forest site at 1000 m further down the trail, suggesting that this taxon may typically occur in Ecuador on ridgetops and/or in a narrow elevational range. Discussion: The only specimens of Cithaerias pyropina that are so far reliably known from Ecuador are those in the type series of C. p. julia. Specimens phenotypically similar to C. p. songoana, with brilliant magenta hindwing patches, occur north of the type locality in Pastaza and Napo provinces at similar elevations to C. p. julia (as figured by D’Abrera (1987: 740) under the name “Cithaerias ereba browni”). However, these specimens lack any of the distinguishing wing pattern or male genitalic (pers. obs.) characters of C. pyropina discussed above under Taxonomy, and are therefore apparently not conspecific with C. pyropina. There is a single male in the FLMNH which appears to be typical C. p. pyropina and which is labeled ‘Mera’ (Pastaza Province), a locality to the north of the Cordillera de Cutucú (marked on Fig. 3 with ‘??’), which would imply that the nominate subspecies should occur in central and southeastern Ecuador, at least in the main Andean Fig. 2: Cithaerias pyropina julia n. ssp., HT♂ genitalia. A. lateral view. B. ventral view. C. dorsal view of anterior tip of aedeagus. differences among subspecies in male genitalic characters. In terms of wing pattern, C. pyropina subspecies share elongate forewings with hardly any or no trace of a brown postdiscal stripe in cells 2A-Cu2 and Cu2-Cu1 in the male. Finally, the only other Cithaerias taxon with bluish DHW iridescence similar to C. p. julia, and which occurs at a similar elevation in the eastern Andes, is C. p. pyropina. Types: HOLOTYPE ♂: Ecuador- Morona-Santiago: Yakunk- Cutucú trail, lower ridge, [2°45’40’’S,78°9’40’’W], 1340 m (K. Willmott), 3 December 2003, [genitalic vial KW-14-83, DNA voucher LEP-14980], 1♂ (to be deposited in Museo Ecuatoriano de Ciencias Naturales, Quito, Ecuador; MECN). PARATYPES (5♂): Ecuador- Morona-Santiago: same locality as HT, 1340-1400 m (K. Willmott), 5 December 2003, 1♂ (Florida Museum of Natural History, Gainesville, FL, USA; FLMNH), 1♂ (The Natural History Museum, London, UK); same data as HT, 3♂ (FLMNH). Etymology: This subspecies is named for Julia Robinson Willmott, in appreciation of her great spirit and excellent company during our trek into the Cutucú, despite her poor health, monotonous food, drenching rain and abysmal mud. The subspecific name is treated as a noun in apposition. Natural history and distribution: Males of C. pyropina julia were not uncommon along the trail through primary cloud forest understorey on the ridge top at the type locality from Fig. 3: Map of the tropical Andes showing the distribution of C. pyropina. ?? 1340-1400 m (Fig. 3B), and a single individual was observed indicates a questionable locality, as discussed further in the text. Inset shows higher up along the same ridge at 1570 m. In a similar manner Cordillera de Cutucú, the type locality for C. pyropina julia n. ssp., and adjacent to other Ecuadorian Cithaerias, they flew low to the ground Ecuadorian Andes, with open circles indicating sampled localities with similar with a steady, gliding flight, weaving among the leaf litter and elevations and habitats to type locality but no records of C. pyropina. 4 TROP. LEPiD. RES., 25(1): 1-4, 2015 WiLLMOTT: A new subspecies of Cithaerias pyropina cordillera. Although ‘Mera’ seems a plausible locality for this species in terms of elevation and habitat, the lack of any other Constantino, L. M. 1995. Revisión de la tribu Haeterini Herrich-Schäffer, 1864 en Colombia (Lepidoptera: Nymphalidae, Satyrinae). Shilap 23: 49- records of C. pyropina from Ecuador, and from northern Peru 76. (G. Lamas, pers. comm.), suggests that the specimen may be Costa, M., Viloria, A. L., Huber, O., Attal, S., Orellana, A. 2013. Lepidoptera mislabeled. We have collected at a number of ridgetop localities del Pantepui. Parte I: Endemismo y caracterización biogeográfica. in the main Andean cordillera and Cordillera del Cóndor, which Entomotropica 28: 193-217. are similar in topography and elevation to the type locality of D’Abrera, B. L. 1987. Butterflies of the Neotropical Region. Part V. C. p. julia, without finding C. pyropina (see Fig. 3). It therefore Nymphalidae (Conc.) & Satyridae. Victoria, Black Rock, Hill House. pp. seems possible that C. pyropina in Ecuador is confined to [viii] + 679-877, figs. the Cordillera de Cutucú, representing a remarkably isolated Duellmann, w. E., Lynch, J. D. 1988. Anuran amphibians from the Cordillera population. de Cutucú, Ecuador. Proceedings of the Academy of Natural Sciences of The Cordillera de Cutucú above 1500 m is still mostly Philadelphia 140: 125-142. primary forest contained within the Bosque Protector Cutucú, which is listed as an Important Bird Area by BirdLife Guayasamín, J. M & E. Bonaccorso (Eds.) 2011. Evaluación Ecológica Rápida de la Biodiversidad de los Tepuyes de la Cuenca Alta del Río International (http://www.birdlife.org/datazone/sitefactsheet. Nangaritza, Cordillera del Cóndor, Ecuador. Conservación Internacional, php?id=14554). Identification of threatened or restricted-range Quito. Available from: http://www.conservation.org.ec/publicaciones/ taxa within the Cordillera could therefore strengthen arguments imagenes/Web%20RAP%20tepuyes.pdf for the conservation of this remarkable region, given the threats Janzen, D. H., Hallwachs, w. 2015. Dynamic database for an inventory of the to the more accessible Cordillera del Cóndor to the south from macrocaterpillar fauna, and its food plants and parasitoids, of Area de the mining industry. Gaining permission to access parts of the Conservacion Guanacaste (ACG), northwestern Costa Rica (nn-SRNP- Cordillera remains difficult, however. Except for our visit in nnnnn voucher codes). http://janzen.sas.upenn.edu 2003 and the pioneering expeditions of Martin Cooper 35 years Lamas, G. 1997. Lepidoptera of the Cordillera del Cóndor, pp. 80-93. In: ago, therefore, nothing else is known of the butterfly fauna of Schulenberg, T.S. & Awbrey, K. (Eds.), The Cordillera del Cóndor this remote and potentially faunistically distinctive mountain region of Ecuador and Perú: a Biological Assessment. Rapid Assessment range. Program, RAP Working Papers 7, Conservation International, Washington DC, USA. ACKNOWLEDGEMENTS Lamas, G. 2004. Nymphalidae. Satyrinae. Tribe Haeterini, pp. 205-206. In: Lamas, G. (Ed.), Checklist: Part 4A. Hesperioidea - Papilionoidea. I thank the museum curators who allowed me to examine In: Heppner, J. B. (Ed.), Atlas of Neotropical Lepidoptera. Volume 5A. the Cithaerias collections under their care, and Gerardo Lamas Gainesville, Association for Tropical Lepidoptera; Scientific Publishers. for his advice and information about Peruvian C. pyropina and Langer, G. 1944. Gattung Callitaera Btlr. (Satyridae, Lep.). Entomologische for making available his type photographs via the “Butterflies Zeitschrift 58(2): 15-16, (3): 19-20. of America” website (www.butterfliesofamerica.com). I thank S. Villamarín, the MECN and Ecuadorian Ministerio del Murillo, L. R. 2009. Early stages and natural history of Cithaerias p. pireta (Satyrinae) from Costa Rica. Journal of the Lepidopterists’ Society 63: Ambiente for arranging the necessary permits for research 169-172. in Ecuador, and Jason Hall for years of collaboration in our Ecuador research. Museum and field work was funded in part Neill, D. A. 2007. Botanical Inventory of the Cordillera del Cóndor Region of by the Leverhulme Trust, the Darwin Initiative, and the National Ecuador and Perú - Project Activities and Findings, 2004–2007. Available from: http://www.mobot.org/mobot/research/ecuador/cordillera/ Science Foundation (# 0847582), and field work by the National Geographic Society (Research and Exploration Grant # 5751- Penz, C. M., Alexander, L. G., DeVries, P. J. 2014. Revised species definitions 96) and NSF (# 0103746, #0639977, #0639861). I thank Martin and nomenclature of the rose colored Cithaerias butterflies (Lepidoptera, Cooper for his helpful correspondence about accessing the Nymphalidae, Satyrinae). Zootaxa 3873: 541-559. Cordillera de Cutucú and for sharing his information about the Radford, J., willmott, K. R. 2013. A distinctive new subspecies of Catasticta butterfly specimens he collected there. I thank Andrew Neild poujadei from the Cordillera del Cóndor in eastern Ecuador (Lepidoptera: and Carla Penz for their very helpful and thoughtful comments Pieridae: Pierinae). Tropical Lepidoptera Research 23: 86-91. on the manuscript. Finally, I thank the many contributors to the Robbins, M. R., Ridgely, R. S., Schulenberg, T. S., Gill, F. B. 1987. The Tropical Andean Butterfly Diversity project butterfly database, avifauna of the Cordillera de Cutucú, Ecuador, with comparisons to other Julia Robinson Willmott for her help and company in the field, Andean localities. Proceedings of the Academy of Natural Sciences of and Galo Mankash for guiding us into the Cordillera de Cutucú Philadelphia 139: 243-259. and for his fine net-skills. Salvin, O., Godman, F. D. C. 1868. On some new species of diurnal Lepidoptera from South America. Annals and Magazine of Natural LITERATURE CITED History (4)2(8): 141-152. Brito M., J., Pozo-Zamora, G. 2013. Una nueva especie de rana terrestre Scoble, M. J. 1992. The Lepidoptera: Form, Function and Diversity. Oxford, del género Pristimantis (Amphibia: Craugastoridae), de la Cordillera de Oxford University Press. xi + 404 pp. Kutukú, Ecuador. Papéis Avulsos de Zoologia. Museo de Zoologia da Universidade de São Paulo 53: 315-325. Schulenberg, T. S., Awbrey, K. (Eds.) 1997. The Cordillera del Cóndor region of Ecuador and Perú: a Biological Assessment. Rapid Assessment Comstock, J. H., Needham, J. G. 1918. The wings of insects. American Program, RAP Working Papers 7, Conservation International, Washington Naturalist 32: 231-257. DC, USA.