ZOOSYSTEMATICA ROSSICA I S S N Zoological Institute, Russian Academy of Sciences, St Petersburg ▪ https://www.zin.ru/journals/zsr/ 2410-0226 [online] Vol. 29(2): 205–212 ▪ Published online 29 September 2020 ▪ DOI 10.31610/zsr/2020.29.2.205 0320-9180 [print] RESEARCH ARTICLE A new species of wolf spiders of the genus Lycosa (Aranei: Lycosidae) from Iran Новый вид пауков-волков рода Lycosa (Aranei: Lycosidae) из Ирана A.A. Nadolny* & A. Zamani А.А. Надольный*, А. Замани Anton A. Nadolny, A.O. Kovalevsky Institute of Biology of the Southern Seas of RAS, Nakhimov Ave. 2, Sevastopol 299011. E-mail: [email protected] Alireza Zamani, Biodiversity Unit, Zoological Museum, University of Turku, Turku, Finland. E-mail: [email protected] Abstract. A large burrowing wolf spider, Lycosa macrophthalma sp. nov., is described from the holotype female from central Iran. The new species can be easily distinguished from all congeners by the presence of anterior epigynal hoods and a protrusion between their edges and the septum. Lycosa macrophthalma sp. nov., L. aragogi Nadolny et Zamani, 2017, L. piochardi Simon, 1876, L. praegrandis C.L. Koch, 1836, and L. tarantula (Linnaeus, 1758) have a similar conformation of the endogyne and represent diagnostic characters of the genus Lycosa Latreille, 1804 sensu stricto. Резюме. Крупный роющий паук-волк Lycosa macrophthalma sp. nov. описан по голотипу (самке) из центрального Ирана. Новый вид отличается от остальных представителей рода наличием передних карманов эпигины и выпуклой области, которая находится между этими карманами и септумом. Lycosa macrophthalma sp. nov., L. aragogi Nadolny et Zamani, 2017, L. piochardi Simon, 1876, L. praegrandis C.L. Koch, 1836 и L. tarantula (Linnaeus, 1758) имеют общую схему строения эндогины, характерную для рода Lycosa Latreille, 1804 sensu stricto. Keywords: wolf spider, Middle East, Iran, Lycosidae, Lycosa macrophthalma, new species Ключевые слова: паук-волк, Ближний Восток, Иран, Lycosidae, Lycosa macrophthalma, новый вид ZooBank Article LSID: urn:lsid:zoobank.org:pub:D9CA35A5-31B5-44ED-88FF-6DE71B329CD0 Introduction visionally identified as “Lycosa tarantula” based on the specimens from France and Spain (Simon, With 2430 species in 123 genera, Lycosidae re- 1937: Figs 1696, 1697; Zyuzin & Logunov, 2000: presents one of the largest spider families. Although Fig. 3, 3A; Logunov, 2010: Figs 1, 27; Oger, 2020). the monophyly of Lycosidae is well supported both Yet, the illustrations of the male and female copu- by morphological characters and molecular data latory organs and general appearance of L. taran- (Dondale, 1986; Wheeler et al., 2017), a clear de- tula, based on the topotypes from Apulia (Italy), lineation of the type genus Lycosa Latreille, 1804 is have been presented only once by Pepe (2005: Figs still problematic due to the enigmatic type species 3–5). Despite the figures in the latter paper being L. tarantula (Linnaeus, 1758) which was original- rather schematic, they clearly show the characters ly described without illustrations. Several authors considered by Zyuzin & Logunov (2000) as the provided illustrations or photos of the spiders pro- main diagnostic characters of the genus: large size; the tongue-shaped septum of the epigyne, with *Corresponding author an elongated anterior part; the male palp with the © 2020 Zoological Institute RAS and the Author(s) A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran broad and asymmetric cymbium and the wide teg- dogyne were made after clearing in a 10% KOH ular apophysis bearing a distal process directed aqueous solution. The length of leg segments was retro-posteriad. Due to the long-term uncertainty measured on the lateral side and listed as follows: regarding the type species, the genus Lycosa cur- total length (femur, patella, tibia, metatarsus, tar- rently includes many medium to large wolf spiders sus). All measurements are given in millimetres. that are not congeneric with L. tarantula. Indeed, For description of spination, the following abbre- with 224 named species from all zoogeographical viations are used in the text: d – dorsal; p – prola- regions (World Spider Catalog, 2020), Lycosa is teral; r – retrolateral; v – ventral. The distribution undoubtedly a polyphyletic taxon. map was created using the SimpleMappr website To date, 73 lycosid species have been recorded (www.simplemappr.net). The holotype has been from Iran, of which four belong to Lycosa (Zamani deposited in the Muséum d’histoire naturelle, et al., 2020; World Spider Catalog, 2020). During Genève, Switzerland (MHNG; curator: Dr. Peter the ongoing taxonomic and faunistic studies of J. Schwendinger). the Iranian spiders, we have found a large female belonging to an undescribed wolf spider species Taxonomy that is a true member of Lycosa. In the Palaearc- tic region, Lycosa is represented by 64 species Order Aranei (World Spider Catalog, 2020) and currently re- Family Lycosidae mains practically unrevised, except for a recent revision of the 12 West Mediterranean species Genus Lycosa Latreille, 1804 (Planas et al., 2013), being based solely on the Lycosa macrophthalma sp. nov. molecular data and completely ignoring morpho- (Figs 1–16, 28, 35–36) logy. Based on the World Spider Catalog (2020), 38 Lycosa species have been recorded from the Holotype. Female, Iran, Isfahan Prov., Ghomishloo Saharo-Gobian Desert Region of the Palaearctic National Park, 32°49′03.7″N 51°13′59.8″E, XI.2017, B. Zadhoush leg. (MHNG). (sensu Emeljanov, 1974) to date. Eight of them are Diagnosis. In the conformation of endogyne, known only from the original descriptions with Lycosa macrophthalma sp. nov. is most similar to no diagnostic illustrations: from South and East L. tarantula, L. praegrandis C.L. Koch, 1836 and Mediterranean countries [L. affinis Lucas, 1846, L. aragogi Nadolny et Zamani, 2017 (Figs 10–12, L. articulata Costa, 1875, L. cingara (C.L. Koch, 32–35), but differs in the shape of the epigyne, 1847), L. intermedialis Roewer, 1955, L. nilotica particularly in having the anterior hoods (Eh) and Audouin, 1826, L. sylvatica (Roewer, 1951)] and protrusion (Ep) between hood edges and a clearly from Yarkand [L. approximata (Pickard-Cam- marked septum (Figs 8, 13–15, 28), compared to bridge, 1885), L. sabulosa (Pickard-Cambridge, no hoods in L. tarantula and L. praegrandis (Figs 1885)]. For the remaining 30 species, at least fe- 17, 23) and the presence of an incision between male copulatory organs were illustrated, allowing hood edges and absence of protrusions on the sides us to undertake a comparison with the new spe- of septum in L. aragogi (Figs. 27). cies from Iran. The present paper is devoted to the Description. Female. Habitus as in Figs 1–6. description of a new Lycosa species having a dis- Total length 18.7; carapace length 9.5, width 6.4. tinct shape of the copulatory organs and hence be- Length of legs: I 23.8 (7.0, 3.5, 5.6, 5.1, 2.6); II 22.3 ing easily distinguishable from all the congeners. (6.4, 3.3, 5.0, 5.1, 2.5); III 22.6 (6.0, 3.0, 4.5, 6.1, 3.0); IV 29.9 (8.0, 3.2, 6.4, 8.9, 3.4). Carapace with Material and methods slightly marked gradual descend of thoracic region, The specimen was photographed using an eye field not elevated (Fig. 6). Carapace brown, Olympus Camedia E-520 camera attached to an covered with white setae forming marginal stripes Olympus SZX16 stereomicroscope at the Zoo- which are well visible in the preserved specimen logical Museum of University of Turku, Finland. (Fig. 3) and poorly distinguishable in vivo (Fig. Digital images were prepared using CombineZP 1). Sternum and labium dark brown, covered with image stacking software. Illustrations of the en- black setae (Fig. 4). Chelicerae brown, proximally 206 ( Zoosystematica Rossica, Vol. 29, No. 2, pp. 205–212 A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran Figs 1–6. Lycosa macrophthalma sp. nov., holotype female. 1–2, live specimen, general appearance and in threat pose; 3–5, habitus in dorsal, ventral, and frontal views; 6, prosoma, lateral view. Scale bars: 2 mm. Photos 1 and 2 by Alireza Zamani. Table 1. Lycosa macrophthalma sp. nov., leg spination. Femur Patella Tibia Metatarsus I d 1-1-1, r 1-1-1, p 2 p 1 p 1-1, v 2-2-2 v 2-2-3 II d 1-1-1, r 1-1-1, p 1-1-1 p 1 p 2-1, v 2-2-2 p 1-1, v 2-2-3 III d 1-1-1, r 1-1-1, p 1-1-1 p 1, r 1 d 1-1, p 1-1, r 1-1, v 2-2-2 p 1-1-1, r 1-1-1, v 2-2-3 IV d 1-1-1, r 1, p 1-1 p 1, r 1 d 1-1, p 1-1, r 1-1, v 2-2-2 p 1-1-1, r 1-1-1, v 2-2-3 covered with white setae, distally with black setae and tarsi: I–IV with spinules, I–II with well-de- (Figs. 2, 5). Palps light brown, with white setae. veloped and III–IV with poorly developed scopu- Legs yellow, covered with white setae. Black setae la. Leg spination (see Table 1). Abdomen yellow, forming spots on all patellae (distally on ventral dorsally with light brown lanceolate cardiac mark and prolateral sides) and tibiae (proximally on and three triangular spots of white and black se- lateral and ventral sides) (Figs 1–2). Metatarsi tae; ventrally with area of black setae tapering to- ( Zoosystematica Rossica, Vol. 29, No. 2, pp. 205–212 207 A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran Figs 7–16. Epigyne and endogyne of Lycosa macrophthalma sp. nov., holotype. 7–9, intact, dissected, and mace- rated epigyne, ventral view; 10–12, macerated endogyne in dorsal, dorso-latero-anterior and dorso-latero-posteri- or views; 13–15, epigyne in posterior, anterior and lateral views; 16, general conformation of endogyne. Cd – copu- latory duct; Co – copulatory opening; Dc – spermatheca duct trajectory; Eh – hood; Ep – protrusion; Es – stalk of septum; Fd – fertilization duct; Hs – head of spermatheca; Os – anterior outgrowth at base of spermatheca; Sp – septum; Ss – stalk of spermatheca. Scale bars: 0.2 mm. ward spinnerets (Fig. 4). Epigyne and endogyne spherical, 1.8 times wider than stalk (Ss), with a as in Figs 7–16, 28, 35. Septum rectangular, with large pore; spherical anterior outgrowth at sper- stalk (Es); hood edges (Eh) rounded; area between mathecal base (Os); copulatory duct (Cd) massive, hoods and septum protuberant (Ep) (Figs 8, 13– zigzag-shaped, situated in front of head of sper- 15); copu latory openings situated near anterior matheca (Figs 10–12, 35). side of septum (Fig. 9); spermathecal head (Hs) Male. Unknown. 208 ( Zoosystematica Rossica, Vol. 29, No. 2, pp. 205–212 A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran Figs 17–35. Female copulatory organs of Lycosa species from the West Palaearctic. 17, 32, L. tarantula; 18, L. fas- ciiventris; 19, L. piochardi; 20, L. vachoni; 21, L. baulnyi; 22, L. munieri; 23, 33, L. praegrandis; 24, L. abnormis; 25, L. oculata; 26, L. bedeli; 27, 34, L. aragogi; 28, 35, L. macrophthalma sp. nov.; 29, L. suboculata; 30, L. hispanica; 31, L. bonneti. Epigynes in ventral view (17–31); endogynes in dorsal view (32–35). After Simon (1937) (17); after Barrientos (2004) (18); after Nentwig et al. (2019) (19); after Guy (1966) (20–22, 24–26, 29); after Simon (1876) (30); after Guy & Carricaburu (1967) (31); after Logunov (2010) (32). Specimens from the Crimea (23, 33). Remarks. The epigyne of L. macrophthalma ca (Walckenaer, 1837), L. munieri Simon, 1876, sp. nov. is similar to that of 14 species from the L. oculata Simon, 1876, L. piochardi Simon, 1876, West Palaearctic (Figs 17–31): L. abnormis Guy, L. praegrandis, L. suboculata Guy, 1966, L. taran- 1966, L. aragogi, L. baulnyi Simon, 1876, L. be- tula, and L. vachoni Guy, 1966. The habitus and deli Simon, 1876, L. bonneti Guy et Carricaburu, the conformation of the epigyne in these species 1967, L. fasciiventris Dufour, 1835, L. hispani- correspond to the diagnostic characters of the ( Zoosystematica Rossica, Vol. 29, No. 2, pp. 205–212 209 A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran Fig. 36. Collecting localities of Lycosa spp. in Iran: L. aragogi (a), L. macrophthalma sp. nov. (b), L. praegrandis (c), L. singoriensis (d), and L. tarantula (e) (Zamani et al., 2020; present data). genus Lycosa (sensu Zyuzin & Logunov, 2000): [L. gobiensis Schenkel, 1936, L. singoriensis (Lax- large spiders with a well-developed long anterior mann, 1770), L. shansia (Hogg, 1912)]; 2) the epi- part of the epigyne and the tongue-shaped epigy- gyne conformation corresponding to the Pardosa nal septum. Other 16 illustrated species of Lycosa nebulosa (Thorell, 1872) species-group [L. terres- recorded from the Saharo-Gobian Desert Region tris Butt, Anwar et Tahir, 2006]; 3) the triangu- (for the references with species descriptions see lar epigyne being similar to that of some mem- World Spider Catalog, 2020) are medium to large bers of Allocosa Banks, 1900 [L. chaperi Simon, spiders having various epigynal conformations, as 1885, L. choudhuryi Tikader et Malhotra, 1980, follows: 1) the anchor-shaped septum being simi- L. trichopus (Roewer, 1960)]; and 4) the peculiar lar to those of Hogna Simon, 1885 [L. cretacea Si- septal shapes that are dissimilar to that of L. ta- mon, 1898, L. interstitialis (Strand, 1906), L. mag- rantula [L. asiatica Sytshevskaja, 1980, L. kempi nifica Hu, 2001, L. nigricans Butt, Anwar et Tahir, Gravely, 1924, L. mackenziei Gravely, 1924]. 2006, L. rufisterna Schenkel, 1953] and Trochosa Lycosa macrophthalma sp. nov., L. arago- C.L. Koch, 1847 [L. maculata Butt, Anwar et Ta- gi, L. piochardi, L. praegrandis, and L. tarantula hir, 2006], or to the species that actually belong have a similar structure of the endogyne, with the to the genus Allohogna Roewer, 1955 but formally massive, curved copulatory duct (Cd) and an out- are still considered in Lycosa (see Logunov, 2010; growth at the base of spermatheca (Os) (cf. Figs World Spider Catalog, 2020: note under Lycosa) 10–12, 32–35; Logunov, 2010: Fig. 27; Nadolny 210 ( Zoosystematica Rossica, Vol. 29, No. 2, pp. 205–212 A.A. Nadolny & A. Zamani. A new species of Lycosa from Iran & Zamani, 2017: Fig. 2 [marked as “massive fold” Dondale C.D. 1986. The subfamilies of wolf spiders and “gland”]; Nentwig et al., 2019: Fig. 6e). In our (Araneae: Lycosidae). In: Barrientos J.A. (Ed.). opinion, such conformation (Fig. 16) is a good Actas X Congreso Internacional de Aracnologia, Jaca (España), septiembre 1986, 1: 327–332. diagnostic character of the genus Lycosa sensu Emeljanov A.F. 1974. Proposals on the classification stricto. and nomenclature of ranges. Entomological Review, It is noteworthy that the diameter of poste- 53(3), 11–26. rior median eyes in this new species is relatively Ghahari H. & Tabari M. 2012. Fauna and popula- large: carapace width/eye diameter ratio in L. tion fluctuations of spiders (Arthropoda: Araneae) macro phthalma sp. nov. is 5.7 (vs. in L. praegran- in rice fields of Mazandaran Province. Journal of dis: from Iran – 6.7, from the Crimea – 9.5, and Plant Protection, 26(2): 136–144. [In Persian] from Rostov Province of Russia – 8.3). Somatic Guy Y. 1966. Contribution à l’étude des araignées de characters (gradually descending carapace pro- la famille des Lycosidae et de la sous-famille des Ly- file, the presence of scopula and spinules on tarsi) cosinae avec étude spéciale des espèces du Maroc. Travaux de l’Institut Scientifique Chérifien et de of L. macrophthalma sp. nov. correspond to the la Faculté des Sciences, Série Zoologie, Rabat, 33: structural and functional features of the burrow- 1–174. ing wolf spiders, as argued by Zyuzin (1990). Guy Y. & Carricaburu P. 1967. Une araignée nouvelle Etymology. The specific epithet is derived from pour la science decouverte en Algerie: Lycosa (Ly- the Greek μακρός (large) and ὀφθαλμός (eye), re- cosa) bonneti n. sp. (Aranea – Lycosidae). Bulletin ferring to the uniquely large posterior median eyes de la Société d’histoire naturelle d’Afrique du Nord, in the new species. 58(3/4): 1–4. Distribution. Known only from the type lo- Logunov D.V. 2010. On new central Asian genus and cality in Isfahan Province, central Iran (Fig. 36). species of wolf spiders (Araneae: Lycosidae) exhi- Both currently known Iranian endemic species of biting a pronounced sexual size dimorphism. Pro- this genus were found in mountains of the Central ceedings of the Zoological Institute of the Russian Academy of Sciences, 314: 233–263. Iranian Range (or Sahand-Bazman Volcanic and Nadolny A.A. & Zamani A. 2017. A new species of Plutonic Belt). It is noteworthy that the single re- burrowing wolf spiders (Araneae: Lycosidae: Lyco- cord of L. tarantula from Iran (Ghahari & Tabari, sa) from Iran. Zootaxa, 4286(4): 597–400. https:// 2012) is highly doubtful and most probably refers doi.org/10.11646/zootaxa.4286.4.13 to L. praegrandis. Nentwig W., Blick T., Gloor D., Jäger P. & Kropf C. 2019. Tackling taxonomic redundancy in spiders: Acknowledgements the infraspecific spider taxa described by Embrik Strand (Arachnida: Araneae). Arachnologische We are grateful to Behzad Zadhoush (Isfahan, Mitteilungen, 58: 29–51. https://doi.org/10.30963/ Iran) for providing us with the collected specimen, and aramit5809 Yuri M. Marusik (Magadan, Russia) and Seppo Ko- Oger P. 2020. Les araignees de Belgique et de ponen (Turku, Finland) for arranging the visit of the France [online]. https://arachno.piwigo.com/ first author to Turku and providing research facilities. index?/category/521-lycosa_tarantula [Updated Vladimir I. Ovtsharenko (New York, USA), Dmitri V. September 2020; Viewed 5 September 2020]. Logunov (Manchester, UK), Yuri M. Marusik and an Pepe R. 2005. Basi zoologiche-naturalistiche del anonymous reviewer provided constructive comments ta rantismo nel Salento. Thalassia Salentina, 27: on an earlier version of the manuscript, and Dmitri V. 47–62. Logunov kindly checked the English of the final draft. Planas E., Fernández-Montraveta C. & Ribera C. The work of A.A. Nadolny was carried out within 2013. 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