2017. journal of Arachnology 45:287-295 A new Liphistius species (Mesothelae: Liphistiidae: Liphistiinae) from Thailand, with notes on its natural history Varat Sivayyapram1, Deborah Roan Smith2, Suthon Weingdow3 and Natapot Warrit!: ’Center of Excellence in Entomology and Department of Biology, Faculty of Science, Ghulalongkorn University, Bangkok 10330, Thailand. E- mail: natapot;[email protected] 2Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, Kansas 66045, USA; 3Mae Wong National Park, Klonglan District, Kamphaeng Phet 62180, Thailand Abstract, A new species of trapdoor spider of the genus Liphistius Schiodte, 1849 (Mesotheiae: Liphistiidae) is described from specimens collected from Mae Wong National Park, Klonglan district, Kamphaeng Phet province, Thailand. This Liphistius species belongs to the bristowei species-group based on the elevated cumulus and the distinct embolic part, and resembles L. yamasakii Ono, 1988. Diagnostic characters of the male and female are discussed, and a map is provided for the type localities of the 32 previously described Liphistius species in Thailand. This is the first record of a Liphistius species in the bristowei species-group that builds a T- or Y-shaped burrow with two trapdoor openings. Keywords: Systematics, checklist, conservation, spider http://zoobank.org/?lsid=urn:lsid:zoobank.org:pub:BlE4838E-2650-4896-88FB-E764C86BlF27 The segmented trapdoor spiders of the Family Liphistiidae male pedipalp—particularly the cumulus and the embolic are the sister group to all other extant spiders. They bear many parts—and the female genitalia. Based on a review of the plesiomorphic characters, including the presence of abdominal literature, the number of species in each species-group in tergal plates and the position of the spinnerets on the median Thailand is as follows: bristowei-grepp (4 species), birmanicus- area of the opisthosoma (Platnick & Gertsch 1976; Haupt group (1 species), trang-group (26 species), and one incertae 2003; Xu et al. 2015). Two allopatric subfamilies are included sedis species, L.jarujini Ono, 1988 (Fig. 1, Table 1). Liphistius in the family: Liphistiinae Thorell, 1869 and Heptathelinae habitats include sloped soil banks on man-made roads or Kishida, 1923. The Liphistiinae includes a single genus, paths cut into mountains or small hills (Haupt 2003). Other Liphistius Schiodte, 1849, found in Laos, Thailand, peninsular Liphistius species dwell in caves and among boulders. Three Malaysia, Myanmar and the Indonesian island of Sumatra types of Liphistius burrows have been documented thus far; (World Spider Catalogue 2017). The Heptathelinae includes however, burrow types do not correspond with species-group the remaining seven genera of liphistiids, whose members are classification. These are: (1) the more or less straight undivided distributed in China, Japan and Vietnam. Heptathelinae can terrestrial tube with one trapdoor opening equipped with silk be distinguished from Liphistiinae by the absence of a male “signal lines” for prey capture; (2) the T- or Y-shaped tibial apophysis and by the female internal genitalia (Platnick terrestrial burrow with two trapdoors, one equipped with & Sedgwick 1984; Haupt 2003; Xu et al. 2015), and by signal lines, the other lacking signal lines; and (3) the sac-like differences in burrow construction (see below). retreat on the surface of a cave wall or boulder, without a The genus Liphistius includes more than 50 described burrowing tube structure but with a trapdoor equipped with species, and in Thailand, 32 Liphistius species have been signal lines (Platnick & Sedgwick 1984; Schwendinger 1990). recorded from 22 provinces throughout the country (Fig. 1, Unlike Liphistius, members of the Heptathelinae do not Table 1), demonstrating a high level of diversity and endemism construct signal lines. (Platnick & Sedgwick 1984; Schwendinger 1987, 1990, 1995, Until now, only one Liphistius species, L. kanthan Platnick, 1996, 1998, 2009, 2013; Ono 1988a, b; Ono & Schwendinger 1997, from Perak, Malaysia, has been evaluated and 1990; Sedgwick & Schwendinger 1990). The group is well designated as a critically endangered species under the IUCN known for its limited dispersal ability, and most Liphistius (2017), showing the possibility of extinction in this group of species described in Thailand have been recorded only from spiders due to restricted ranges, habitat destruction and their type localities. Currently, only seven Thai Liphistius human exploitation. In August 2015, the authors (VS and species are known to occur outside of their type localities: L. NW) discovered an aggregation of Liphistius burrows along a bicoloripes Ono, 1988, L. bristowei Platnick & Sedgwick, 1984, man-made road cutting during a spider collecting expedition L. isan Schwendinger, 1998, L. iahu Schwendinger, 1988, L. to Mae Wong National Park, Thailand. Here, we describe this lannaianus Schwendinger, 1990, L. pusohm Schwendinger, species for the purpose of further ecological studies and for 1996, and L. thaleban Schwendinger, 1990. However, the conservation assessment. apparently limited distributions of many Liphistius species could also be a result of insufficient sampling, habitat METHODS alteration, and deforestation. Schwendinger (1990) classified Liphistius into three species- Spiders were collected in Mae Wong National Park, groups: the bristowei species-group, the birmanicus species- Klonglan district, Kamphaeng Phet province, Thailand group, and the trang species-group, based on characters of the (16°05.67'N, 99°07.436'E) on a man-made road cutting (Fig. 287 288 JOURNAL OF ARACHNOLOGY November 2015 (for specimens collected in August 2015) and until 25 December 2015 (for specimens collected in December 2015) before being preserved in 95% ethanol. Measurements are reported in millimeters and were obtained using a Zeiss Stemi DV4 stereomicroscope with ocular micrometer, or with digital calipers. Total length (with and without chelicerae length) does not include the anal tubercle. Appendage measurements were based on the left appendages. Pedipalp and leg lengths include the lengths of the femur, patella, tibia, metatarsus, and tarsus. The female genital area was removed from the specimens and cleared using 5% potassium hydroxide. Terminology of genital characters follows Schwendinger (1987, 1990, 1995, 1996, 1998, 2009) and Schwendinger & Ono (2011). The abbrevia¬ tions used here are as follows: ALE, anterior lateral eye; AME, anterior median eye; CL, carapace length; CW, carapace width; EL, palpal coxal length; EW, palpal coxal width; LL, labium length; LW, labium width; OL, ocular tubercle length; OW, ocular tubercle width; PME, posterior median eye; PLE, posterior lateral eye; SL, sternum length; SW, sternum width; TL|, total length with chelicerae; TL2, total length without chelicerae. SYSTEMATICS Family Liphistiidae Thorell, 1869 Subfamily Liphistiinae Thorell, 1869 Genus Liphistius Schiodte, 1849 Liphistius maewongensis sp. nov. http://zoobank.org/?lsid=urn:lsid:zoobank. org:act:93009B75-A775-4376-B2AF-C8C421 OF 1F15 (Figs. 2-6) Type material.—Holotype male. THAILAND: Kamphaeng Phet: Klonglan District, Mae Wong National Park, Figure 1.—Type localities of 33 described Liphistius species in 16°05.67'N, 99°07.436'E), 1266 m, 23 December 2015, V. Thailand based on descriptions from Platnick & Sedgwick (1984), Sivayyapram (CUMZ-AR-ARA-Lip.2017.1). Schwendinger (1987, 1990, 1995, 1996, 1998, 2009), Ono (1988a, b), Paratypes. THAILAND: Kamphaeng Phet: 1 2 allotype, Ono & Schwendinger (1990), and Sedgwick & Schwendinger (1990). same data as holotype except 6 August 2015 (CUMZ-AR- See Tabic 1 for type locality information regarding each species. The ARA-Lip.2017.2); 3 2, same data (CUMZ-AR-ARA- species displayed is a female L. erawan Schwendinger, 1996 (dorsal Lip.2017.3); 1 2, same data except 23 December 2015 view). (CUMZ-AR-ARA-Lip.2017.4); 1 2, same data (CUMZ- AR-ARA-Lip.2017.5); 1 2, same data (CUMZ-AR-ARA- Lip.2017.6). 2). An estimated 300+ burrows were present on the banks at Other material examined. —THAILAND: Kamphaeng Phet: the time of discovery (August 2015). We excavated a subset of 5 2, 7 subadult juveniles, Klonglan District, Mae Wong the burrows using forceps and collected 15 specimens (7 2,8 National Park, 16°05.67'N, 99°07.436'E, 1266 m, 6 August 2015, V. Sivayyapram (CUMZ-AR-ARA-Lip.2017.7-18); 14 subadults). Trapdoor width and length, and burrow depth 2, 12 subadult juveniles, same data except 16°05.333'N, were measured for each specimen using digital calipers 9 9 °0 7.8 8 'E ), 119 3 m, 23 December 2015 (Mitutoyo). Numbers of signal lines were also recorded. One (CUMZ-AR-ARA-Lip.2017.19-44); 2 subadult juveniles, of the subadult specimens collected molted into an adult male same data except 1218 m (CUMZ-AR-ARA-Lip.2017.45-46). on 30 October 2015 (this specimen is designated as the Etymology.—The specific epithet refers to the type locality, holotype). In late December 2015, we returned to the initial Mae Wong National Park, an important tiger sanctuary in collecting site and discovered two additional collecting Southeast Asia. localities 1 km east and west of the original location Diagnosis.—Liphistius maewongensis sp. nov. is similar to L. (16°05.333'N, 99°07.88'E; 16°05.334'N, 99°07.88'E) and col¬ yamasakii Ono, 1988, but can be distinguished from the latter lected an additional 31 specimens (17 2, 14 subadults). Eleven by its smaller size, by the distinct shape of the embolus, which egg sacs were also recovered from the female burrows. All live has a slender tip (Fig. 4b), by the less prominent sharp distal specimens were transported back to the Department of edge of the contrategulum (Fig. 4a), by the narrower Biology, Chulalongkorn University, and reared until 1 paracymbium (Fig. 4a, b), by the less prominent cumulus SIVAYYAPRAM ET AL.—A NEW LIPHISTIUS SPIDER FROM THAILAND 289 Tabic 1.—Synopsis of 33 described Liphistius species from Thailand. Type localities arc provided verbatim from their original descriptions. Remarks are our interpretation and additions. Liphistius species-group classification is based on Schwcndinger (1990). The species numbers correspond to Figure 1. Original Species- No. Species Type Locality Type Locality Remarks Description group 1 L. lalut “Doi Angkhang (19°57'N, 99°05'E), Schwcndinger birmanicus 1500 m alt., Fang District, Chiang (1998) Mai Province, northern Thailand.” 2 L. lannaianus “Thailand: Huay Nam Dang and Doi Doi Chang is the highest peak in Huai Schwcndinger bristowei Chang” Nam Dang National Park (1,962 m), (1990) Mae Taeng District, Chiang Mai province 3 L. bristowei “Doi Suthep mountain, 1,100 m alt., Platnick & bristowei Chiang Mai, Thailand” Sedgwick (1984) 4 L. yamasakii “Doi Inthanon, 1,700 m alt. between Ono (1988a) bristowei Maeo Khun Klang and Mae Chaem. Chiang Mai, Thailand” 5 L. marginatus “Thailand: Lan Sang National Park" Lan Sang National Park, Mucang Schwcndinger bristowei District, Tak province (1990) 6 L. maewongensis Kamphaeng Phet province, Klonglan Sivayyapram et al. bristowei n. sp. District, Mae Wong National Park (2017) (16° 05.670'N, 99° 07.436'E), elevation 1266 meter 7 L. owadai “Thung Salaeng Luang, 550 m alt., Ono & trang Phitsanulok Province, Thailand” Schwcndinger (1990) 8 L. isan “Phu Phan National Park (16°43'N, Schwcndinger trang 103°51'E), 520 m, Kut Bak District, (1998) Sakon Nakhon Province, northeastern Thailand.” 9 L. dangrek “Phu Chong Nayoi National Park Schwcndinger (1996) indicated that L. Schwcndinger trang (14°18'N, 105°10'E), 300 m, Nam Yun dangrek is found only in the (1996) District, Ubon Ratchathani Province, surrounding of Tham Bak Tew northeastern Thailand.” Waterfall in the NP. Currently, this waterfall has changed its name to “Huai Luang Waterfall" 10 L. suwat “Hco Suwat Waterfall (14°21'N, Schwcndinger trang 101°29'E), 580 m, Khao Yai National (1996) Park, Pak Chong District, Nakhon Ratchasima Province, northeastern Thailand.” 11 L. nesioticus “Ko Chang National Park (12°01'N, Koh Chang district was separated from Schwcndinger trang 102°19'E), 50 m, Lacm Ngop District, Laem Ngop district in 2007 and is the (1996) Trat Province, southeastern type locality of L. nesioticus Thailand.” 12 L. say am “Khao Khieo Wildlife Sanctuary Schwcndinger trang (13°10'N, 100°58'E), 110 m. Si Racha (1998) District, Chon Buri Province, central Thailand” 13 L. ornatus “Khao Soi Dao Wildlife Sanctuary, Ono & trang 300-400m alt., Chanthaburi Province, Schwcndinger Thailand” (1990) 14 L. phileion “Ao Phrao (=Coconut Bay), 10 m, Schwcndinger trang Samet Island (12°33'N, 101°26'E), (1998) Khao Laem Ya & Mu KO Samet National Park, Rayong District and Province, southeastern Thailand." 15 L. ochraceus “Phu Rua National Park, 1,200m alt.. Ono & trang Loci Province, Thailand" Schwcndinger (1990) 16 L. onoi “Phu Hin Rongkla National Park Schwcndinger trang (16°52'N, 10l°03'E), 1200 m, Nakhon (1996) Thai District, Phitsanulok Province, northeastern Thailand" 290 JOURNAL OF ARACHNOLOGY Table 1.—Continued. Original Species- No. Species Type Locality Type Locality Remarks Description group 17 L. thoranie “Kong Kaeo Waterfall (14°21'N, Schwendinger trang 101°25'E), 680 m, Khao Yai National (1996) Park, Pak Chong District, Nakhon Ratchasima Province, northeastern Thailand.” 18 L. thorn “Thailand, Tham Suan ft in"' “Tham Suan Hin” described by Sedgwick & trang Sedgwick and Schwendinger (1990) is Schwendinger currently known as Lumphinee Suan (1990) Hin cave, Wat Tham Phra Phothisat monastery, Kaeng Kfaoi District, Saraburi province (Ellis 2012) 19 L. pusohm “Nam Nao National Park (16°44'N, Schwendinger trang 101°32'E), 800 m Lom Sak District, (1996) Phetchabun Province, northeastern Thailand.” 20 L. erawan “Erawan Waterfall and National Park Schwendinger trang (14°25'N, 99°03'E), 100 m. Bo Phloi (1996) District, Kanchanaburi Province, western Thailand” 21 L. schwendingeri “Kfalong Nakha, 50m alt., Ranong, Khlong Na Kha Wildlife Sanctuary, Suk Ono (1988b) trang South Thailand” Samran district, Ranong province 22 L. fuscus “Khao Phanom Bencha National Park Schwendinger trang (8°12'N, 98°56'E), 280 m, Krabi (1995) District, Krabi Province, Thailand.” 23 L. phuketensis “Tone Sai Waterfall, Khao Phra Thaeo Schwendinger trang Non-hunting Area (8°02'N, 98°22'E), (1998) 100 m, Thalang District, Phuket Island and Province, southern Thailand.” 24 L. rufipes “Than To Waterfall (6°2'N, IQHO'E), Schwendinger trang 150 m, Banglang National Park, Than (1995) To District, Yala Province, Thailand.” 25 L. thaleban “Thailand: Thaleban National Park” Thale Ban National Park, Wang Schwendinger trang Prachan, Khuan Don District, Salun (1990) province 26 L. bicolorip.es “Khlong Nakha, 50m alt, Ranong, Khlong Na Kha Wildlife Sanctuary, Na Ono (1988b) trang South Thailand” Kha, Suk Samran District, Ranong province 27 L. castaneus “Khlong Nakha Wildlife Sanctuary, 30 Schwendinger (1995) might have erred Schwendinger trang m, Kapoe District, Ranong Province, in stating that Khlong Nakha WS is (1995) Thailand.” in Kapoe district, since this WS is located in Suk Samran district 28 L. niphanae “Khao Luang National Park, 120 m Ono (1988b) trang alt.. Nop Pitam, Tha Sala, Nakon Si Thammarat, South Thailand” 29 L. trang “Krachong Forest, 100 m. near Trang, Krachong Forest, Na Yong District, Platnick & trang Thailand” Trang Province Sedgwick (1984) 30 L. thaleri "¥(cid:9632) o {= Island) Libong (also called Ko Schwendinger trang Talibong), near Ao Tokae (= Gekko (2009) Bay) (7°16'04"N, 99°22'38"E), 30 m” 31 L. albipes “Khao Luang, Nam Tok Huay Yang Schwendinger trang National Park (11°38'N, 99°33'E), 550 (1995) m, Thap Sakae District, Prachuab Khiri Khan Province, Thailand.” 32 L. tenuis “Nam Tok Phliu-Khao Sabap National Schwendinger trang Park (12°32'N, 102°12'E), 100 m, (1996) Chanthaburi District and Province, southeastern Thailand.” 33 L. jarujini “Taksin Maharat National Park, 950 m Ono (1988a) Incertae alt, ca 30 km W of Tak, Muzng, sedis Thailand" SIVAYYAPRAM ET AL.—A NEW LIPHISTIUS SPIDER FROM THAILAND 291 Figure 2.—Type locality of Liphistius maewongensis sp. nov. in Mae Wong National Park (a, b), Klonglan District, Kamphaeng Phet province, Thailand; (c), a man-made road cutting hill slope where L. maewongensis burrows were found. Figure 3.—Dorsal habitus of Liphistius maewongensis sp. nov. Adult male (left) and female (right). Note the darker coloration of the male specimen. 292 JOURNAL OF ARACHNOLOGY AME-PME 0.09, AEL-PLE 0.06. Labium: LL 0.72, LW 1.36. Sternum: SL 3.08, SW 0.96. Palpal coxa: EL 1.96, EW 1.60. Chelicerae with 11 promarginal teeth. Paired tarsal claws with 4 teeth, unpaired tarsal claw with 1 small denticle. Pedipalp and leg measurements: pedipalp length: 11.36 (3.60+2.08+3.68+2.00), leg I: 17.12 (4.88+2.56+3.68+3.92+2.08), leg II: 15.00 (2.28+2.56+3.52+4.40+2.24), leg III: 19.92 (5.28+2.56+3.44+5.54+2.80), leg IV: 25.12 (6.32+2.88+4.96+7.12 +3.84). Pedipalp with four tapering spines on short truncate tibial apophysis, paracybium protruding and narrow bearing numerous short strong spines, cumulus elevated bearing several long hairs, alveolar process well developed, subtegular apophysis slightly elevated, tegulum narrow with dentate dorsoproximal edge of tegulum, contrategulum broad with dentate ventral ridge, small paraembolic plate. Embolus short, adjoining sclerotized embolic parts with two longitudinal ridges that reach to tip (Fig. 4). Female allotype (CUMZ-AR-ARA-Lip.2017.2). Color (live specimens): carapace orange-brown with thick black markings along the margins which radiate to thoracic groove; opistho¬ soma orange-brown with black spot on lateral surface, abdominal tergites orange-brown with black pattern in the middle and lateral margins (Fig. 3); chelicerae brown proximally, black distaliy; leg and pedipalp light-brown with black annulations; palpal coxa, leg coxae, labium, and sternum black. Total length: TL1 12.38, TL2 10.63. Carapace: CL 5.88, CW 5.38. OcuUr tubercle: OL 0.88, OW 0.88, clypeus narrow. Eye sizes and interdistances: AME 0.09, ALE oval shape 0.51 and 0.15, PM E 0.39, PLE oval shape 0.42 and 0.12, AME-AME 0.06, AME-ALE 0.12, PME-PME 0.03, PME- PLE 0.06, ALE-PLE 0.09. Labium: LL 0.76, LW 1.12. Sternum: SL 3.04, SW 1.32. Palpal coxa: EL 1.92, EW 1.16. Chelicerae with 12 promarginal teeth. Paired tarsal claws with 3 teeth, unpaired tarsal claw with 1 small denticle. Pedipalp Figure 4.—Male pcdipalp of Liphistius maewongensis sp. nov. (holotype: CUMZ-AR-ARA-Lip.2017.1): (a) retrolateral view; (b) and leg measurements: pedipalp length: 10.56 retroventral view; (c) ventral view; (d) provcntral view; (e) distal view. (3.68+1.92+2.40+2.56), leg I: 13.36 (4.48+2.08+2.72+2.56+1.52), A = alveolar apophysis; CT = contrategulum; Cu = cumulus; Cy = leg II: 13.52 (4.40+2.08+2.80+2.64+1.60), leg III: 14.64 cymbium; DT = dorsal extension of terminal apophysis of tegulum; E (4.40+2.08+2.88+3.36+1.92), leg IV: 20.80 (5.68+2.56+4.08+5.60 = embolus; PC = paracybium; PeP = paracmbolic plate; SE = sharp +2.88). Vulva: anterodorsal poreplate rectangle, wider than long, distal edge of contrategulum; ST = subtcgulum; TiA = tibial anterior and lateral lips thick without distinct lobe, large ventral apophysis. vesicle (Fig. 5). Receptacular cluster racemose, well develop. Genital atrium wide, sclerotized portion well developed, with W- shaped posterior margin, connected to lateral margin of atrium. Variation. -Pedipalp and leg measurements for nine adult (Fig. 4a, b), and by the distinct subtegular apophysis (Fig. 4b, female specimens (individuals with egg sacs collected on 23 c) (see also Ono 1988a). The female L. maewongensis has December 2015) are provided in Table 2. larger medium-sized pores on the pore plate that lead to the Remarks.Liphistius maewongensis belongs to the bristowei ampulliform vesicles, and a distinct genital atrium and species-group sensu Schwendinger (1990), based on the arrangement of the receptacular cluster (Fig. 5). elevated cumulus (Figs. 4a-d) and the two longitudinal ridges Description. -Male holotype (CUMZ-AR-ARA- on the sclerotized part of the embolic parts that reach the tip Lip.2017.1). Color (live specimens); carapace black, paler in (Fig. 4e). Thus, the bristowei species-group of Liphistius is median area; opisthosoma black, abdominal tergites darker currently comprised of L. bristowei, L. lannaianus, L. margin- than other areas; chelicerae black, paler in proximal portion; atus Schwendinger, 1990, L. yamasakii, and L. maewongensis. leg and pedipalp pale yellow with black annulations; palpal Despite the similarity between L. yamasakii and L. maewon¬ coxa, leg coxae, labium and sternum black (Fig. 3). Total gensis, the male pedipalp shapes are grossly different (see length: TL1 16.3, TL2 14.7. Carapace: CL 6.75, CW 6.25. Diagnosis, above). Ocular tubercle: OL 0.99, OW 1.17. Eye sizes and interdis¬ Distribution, burrows and natural history.—Liphistius mae¬ tances: AME 0.9, ALE oval shape 0.18 and 0.75, PME oval wongensis is found only in the area surrounding the type shape 0.42 and 0.33, PLE oval shape 0.18 and 0.48; AME- locality in Mae Wong National Park, which spans an area of AME 0.15, AME-ALE 0.18, PME-PME 0.03, PME-PLE 0.09, approximately 2-3 km2 within an altitudinal range of 1193- SIVAYYAPRAM ET AL.—A NEW LIPH1STIUS SPIDER FROM THAILAND 293 Figure 5.—Female vulvae of Liphistius maewongensis sp. nov. (a-c) Allotype (CUMZ-AR-ARA-Lip.2017.2): (a) dorsal view; (b) ventral view; (c) distal view; (d) Variations in four female paratype vulvae (upper row, dorsal view; lower row, ventral view), from left to right (CUMZ-AR- ARA-Lip.2017.3-6, respectively). CDO = central dorsal opening; GA = genital atrium; PP1 = porcplate; RC = receptacular cluster; V = ampulliform vesicle. 1266 m. No additional individuals were found beyond 10 km 46), and an average depth of 69.02 ± 23.84 mm (24.66-144.5 from the type locality. Liphistius maewongensis was found in mm; n =46). The shape of trapdoors of straight burrows with soil burrows on man-made road cuttings with a gentle surface signal lines was more or less oval with an average width of inclination of 68.2-87.1 degrees measured from ground level. 10.02 ± 4.97 mm (4.6-24 mm; n = 21), an average length of All burrows, regardless of size, had a trapdoor. Two types of 14.84 ± 6.37 mm (7-28 mm; n = 21), and an average depth of burrows were observed, the simple straight burrow with a 70.26 ± 21.02 mm (40-105.1 mm; n = 21). The shape of single opening (with signal lines) (n = 21) and the T- or Y- trapdoors of T- or Y-shaped burrows with signal lines was shaped burrow (n = 25) with two openings (one trapdoor is more or less ova! with an average width of 12.14 ± 2.77mm equipped with signal lines, whereas the other is not) (Fig. 6). (8.1-17.9 mm; n = 25), an average length of 17.74 ± 3.63 mm The number of signal lines on trapdoors ranged from 4-8 (n = (12.3-27.3 mm; zz=25), and an average depth of 67.97 ± 27.74 46). Straight burrows had an average number of signal lines of mm (27.6-144.5 mm; n =25). The shape of trapdoors of T- or 5.86 ± 1.01 (4-7; n = 21), whereas T- or Y- shaped burrows Y-shaped burrows without signal lines was more or less oval had an average number of signal lines of 6.24 ± 0.88 (5-8; n = with an average width of 9.6 ± 2.42 mm (5.1-15.5 mm; n = 25) 25). The shape of trapdoors with signal lines was more or less and an average length of 12.92 ± 2.67 (9.5-18.8 mm; n = 25). oval with an average width of 11.17 ± 4.03 mm (4.6-24 mm; n In the field, we observed that while excavating for individual = 46), an average length of 16.42 ± 5.21 mm (7-28 mm; n = spiders in a T- or Y-shaped burrow through its trapdoor with 294 JOURNAL OF ARACHNOLOGY Figure 6.—Burrow types of Liphistius maewongensis sp. nov.: (a) two trapdoor openings of a T- or Y-shaped burrow (note the opening without signal lines used as escape door; yellow arrow); (b) a cross-sectional sketch of a T- or Y-shaped burrow; (c) an opening of a trapdoor of a simple horizontal burrow with signal lines; (d) a cross-sectional sketch of a simple horizontal burrow. signal lines, the spider frequently escaped through the second ACKNOWLEDGMENTS trapdoor without signal lines. We speculated that one of the The authors are grateful to the assistance in the field provided functions for the second trapdoor is that it is used as an by the park rangers at Mae Wong National Park. Our “escape door” (Fig. 5a). This is the first report of a T- or Y- colleagues, Nontawat Chatthanabun, Narin Chomphuphuang, shaped burrow found in a species belonging to the bristowei Sirat Lertjintanakit, Pakorn Nalinrachatakan and Chaowalit species-group, since Schwendinger (1990) earlier reported that Songsangchote assisted us during intensive field collecting such a burrow characteristic is usually only found in the throughout the study. Peeranat Bokoranee and Puttita Pasukdee binnanicus- and /rang species-groups. assisted us with the figures of the male and female genitalia and The number of eggs per egg sac was between 31-52 eggs (n = the Thailand map. We also greatly appreciate Michael Rix, Peter 11). Since one male of L. maewongensis molted to an adult in Schwendinger and an anonymous reviewer for providing useful October, and high numbers of female egg sacs were found in comments to improve the manuscript. This research is supported December and not in August, we inferred that the mating by the 90th Anniversary of Chulalongkorn University Scholar¬ period is likely to be around October-December. This annual ship (GCUGR1125601047M) and the Graduate School schol¬ cycle concurs with the findings of Schwendinger (1990) for arship, Chulalongkorn University to commemorate the 72nd species of the bristowei and binnanicus species-groups, which anniversary of his Majesty King Bhumibol Adulyadej. The also have mating seasons during this time in Thailand. Center of Excellence in Biodiversity, Office of Higher Education Tabic 2.—Pcdipalp and legs measurements of nine adult females of Liphistius maewongensis sp. nov. collected on 23 December 2015. All measurements (mean ± s.d.) are given in millimeters. Femur Patella Tibia Metatarsus Tarsus Total length Pedipalp 3.34 ± 0.52 1.73 ± 0.41 2.34 ± 0.44 _ 2.35 ± 0.40 9.76 ± 1.69 Leg I 4.06 ± 0.57 2.07 ± 0.30 2.51 ± 0.43 2.38 ± 0.40 1.38 ± 0.18 12.39 ± 1.81 Leg 11 4.10 ± 0.62 2.04 ± 0.32 2.53 ± 0.43 2.54 ± 0.36 1.42 ± 0.30 12.64 ± 1.92 Leg III 4.06 ± 0.59 2.06 ± 0.33 2.64 ± 0.41 2.96 ± 0.67 1.74 ± 0.25 13.45 ±2.17 Leg IV 5.17 ± 0.77 2.39 ± 0.32 3.60 ± 0.47 5.09 ± 0.82 2.54 ± 0.36 18.79 ± 2.68 S1VAYYAPRAM ET AL.—A NEW LIPHISTIUS SPIDER FROM THAILAND 295 Commission, Thailand also provided partial funding for this Schwendinger, P.J. 1990. On the spider genus Liphistius (Araneae: work (PERDO-BDC: BDC-PG2-159009/1). Mesothelae) in Thailand and Burma. Zoologica Scripta 19:331 351. Schwendinger, P.J. 1995. New Liphistius species (Araneae, Mcso- LITERATURE CITED thclac) from southern Thailand and northern Malaysia. Zoologica Scripta 24:143-156. Ellis, M. 2012. The Caves of Saraburi, Thailand. 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