3rd Symposium on Harmful Algae in the U.S. Symposium Director: Chris Scholin Symposium Coordinators: Judy Kleindinst, Annette Gough, Mary Arnold, Jeannette Fink Steering Committee: Greg Boyer State University New York – Environmental Science and Forestry Quay Dortch NOAA, National Ocean Service, Silver Spring Greg Doucette Marine Biotoxins Program, NOAA/National Ocean Service Pat Glibert Horn Point Laboratory Cindy Heil MYFWC Raphe Kudela Ocean Sciences Department, University of California, Santa Cruz Kevin Sellner Chesapeake Research Consortium Marc Suddleson NOAA Ocean Service/CSCOR Vera Trainer NWFSC Tracy Villareal University of Texas at Austin Session Chairs: Bloom Ecology Kevin Sellner, Raphe Kudela, Quay Dortch Toxins: Greg Boyer, Greg Doucette Foodwebs: Cindy Heil, Vera Trainer Public Health: Tracy Villareal, Pat Glibert Outreach/Infrastructure: Marc Suddleson, Chris Scholin Sponsors: Monterey Bay Aquarium Research Institute NOAA/Center for Sponsored Coastal Ocean Research/Coastal Ocean Program U.S. National Office for Marine Biotoxins and Harmful Algal Blooms Student support: NOAA/Center for Sponsored Coastal Ocean Research/Coastal Ocean Program West Coast Center in Oceans and Human Health Center of Excellence for Oceans and Human Health at the Hollings Marine Laboratory Front Cover: A 3-D view of a phytoplankton layer (chlorophyll fluorescence) dispersed along the crest and concentrated in the trough of an internal wave (light blue isopycnal), observed at high resolution using an AUV (Ryan et al. 2005, Mar. Ecol. Prog. Ser. 287:23-32). The layer of phytoplankton contained Pseudo-nitzschia australis, a toxigenic diatom linked to illness and mortality of marine wildlife (Scholin et al. 2000, Nature 403: 80- 84). Source populations of organisms that ultimately give rise to HABs in coastal areas may occur offshore and be subsurface, sometimes in thin layers, and therefore are often difficult to detect using traditional ship surveys and even remote sensing. These blooms can be delivered to near shore areas by physical forcing, resulting in sudden increases in toxicity that are unrelated to local growth. n siteendeeking PosterSessions iAcacia,Heather, &Toyon OffAttPar DisabledParking .evA r a m o lis A s y a w h at P s n o atiel tp na eh alesC OrPrn i Bonfire Pit r Ave. a m o sil A ESTGE RD OO FL Surf and Sand nall oH nereptirill DinRecMer October 2005 3rd Symposium on Harmful Algae in the U.S. *Pre-registration on Sunday, October 2 from 3:00pm - 5:00pm at Asilomar, Acacia room. Refreshments will be served.* Schedule Mon., Oct. 3 Tues., Oct. 4 Wed., Oct. 5 Thu., Oct. 6 7:30am-8:30am Breakfast Breakfast Breakfast Breakfast 8:30 am Welcome and (First day only) Introduction 8:40 am-10:00 am Session 1 Session 5 Session 9 Session 13 HAB plan Toxins Bloom Ecology Bloom Ecology overview Outreach and Infrastructure Break 10:00 am - 10:30 am 10:30am-11:50am Session 2 Session 6 Session 10 Session 14 Outreach and Toxins Bloom Ecology Bloom Ecology Instrastructure Lunch 12:00pm - 1:30pm 1:30pm - 2:30pm Session 3 Session 7 Session 11 Session 15 Outreach and Food Webs Bloom Ecology Bloom Ecology Infrastructure Break 2:30pm - 3:00pm 3:00pm - 4:00pm Session 4 Session 8 Session 12 Session 16 Toxins Public Health Bloom Ecology Bloom Ecology Business Mtg Site selection for next meeting 4:00pm-6:00pm Public Outreach Fisheries and Public Health & Poster Session & & Toxins Food Web Bloom Ecology Refreshments (Heather Meeting (Acacia Meeting (Toyon Meeting Room) Room) Room) Dinner Reception Dinner 6:00pm 6:00pm (Merrill Hall) NOAA Event West Coast 8:00pm-10:00pm Response - Collaborations Special Evening M. Suddelson w/Emphasis on Sessions Pseudo-nitzschia - P. Miller & V. Trainer Presentation Schedule 3 3rd Symposium on Harmful Algae in the U.S. October 2005 Monday, October 3, 2005 Session 1: Outreach and Infrastructure Time Presenter Title 8:30 Scholin, Chris Welcome and opening Remarks (8:30-8:40) 8:40 Glibert, Patricia HAB plan overview 9:00 Marsh, Anne S. A National Harmful Algae Indicator to Monitor the Condition of Coastal Waters in the United States 9:20 Kirkpatrick, Barbara The START Story: Expansion From a Small Local Effort to Statewide Efforts and Benefits to the HAB Community 9:40 Morton, Steve Utilization of Volunteers to Monitor Harmful Algal Blooms: The Southeastern Phytoplankton Monitoring Network Break 10:00 am - 10:30 am Session 2: Outreach and Infrastructure 10:30 Stumpf, Richard P. Remote Sensing Detection of Red Tides: and Other Harmful Algal Blooms that Discolor the Water 10:50 Campbell, Lisa Buoy-Based in Situ Imaging System for Real-Time Monitoring of Karenia brevis in the Gulf of Mexico 11:20 Haywood, Allison J. Molecular Detection of Karenia brevis and Related Species Using Sandwich Hybridization Assays 11:30 Connell, Laurie Filling the Bloom Monitoring and Research Resource needs of the HAB Community Lunch 12:00pm - 1:30 pm Session 3: Outreach and Infrastructure 1:30 Dalgleish, Fraser Remote Imaging System for Monitoring Macroalgal HABs in Deep Reef Communities Off Southeast Florida New Solid-State Fluorescence Sensor Used to Monitor Donovan, Chelsea Photosynthetic Parameters 1:50 An Epidemiologic Study of the Aerosolized Florida Red Fleming, Lora E. Tide Toxins on Asthmatics 2:10 Break 2:30 pm - 3:00 pm Session 4: Toxins 3:00 Gulland, Francis Environmental Exposures to Florida Red Tides: Effects on Emergency Room Respiratory Diagnoses Admissions 3:20 Landsberg,Jan Saxitoxin Monitoring in Florida: One More Toxin to Deal With 3:40 Radwan, Faisal F.Y. Identification of a Rapid Detoxification Mechanism for Brevetoxin in Rats 4 Presentation Schedule October 2005 3rd Symposium on Harmful Algae in the U.S. Tuesday, October 4, 2005 Session 5: Toxins Time Presenter Title 8:40 Van Dolah, Frances Functional Genomic Studies in Karenia brevis: Current Insight into Mechanisms Regulating Growth, Toxicity and Adaptation 9:00 Monroe, Emily Brevetoxin and Polyketide Synthase Gene Expression Under Low-Nutrient Conditions in the Dinoflagellate, Karenia brevis 9:20 Bachvaroff, Tsvetan Linking Genetic Differences Between Karlodinium micrum strains with differences in toxin type and abundance 9:40 Villareal, Tracy Growth and Toxicity of the Dinoflagellate, Gambierdiscus Toxicus, Under Nitrogen and Phosphorus Limitation Break 10:00 am - 10:30 am Session 6: Toxins 10:30 Place, Allen R. The Toxin from Gymnodinium veneficum Ballantine - Rediscovered: It’s a Karlotoxin 10:50 Armstrong, Meredith The Production of Yessotoxin in California Isolates of Lingulodinium polyedrum 11:20 Goldberg, Judah Recurrent Presence of Pseudo-nitzschia and Domoic Acid in a Pacific Northwest Estuary 11:30 Vogelbein, Wolfgang Determinants of Pathogenicity in Pfiesteria piscicida & Pseudopfiesteria shumwayae: Species and Strain Comparisons Lunch 12:00pm - 1:30 pm Session 7: Food Webs 1:30 Demir, Elif Microzooplankton Grazing on Heterosigma akashiwo in Delaware Inland bays, and Application of QRT-PCR Technique 1:50 Flewelling, Leanne Unexpected Vectors of Brevetoxins During Marine Mammal Mortalities 2:10 Foster, Vicki Allelopathic Effects of Karlodinium micrum on Co- Occurring Dinoflagellates Break 2:30pm - 3:00pm Session 8: Public Health 3:00 Backer, Lorraine Environmental Exposures to Florida Red Tides: Effects on Emergency Room Respiratory Diagnoses Admissions 3:20 Jellett, Joanne F. Enhancing Public Health and Safety Through Distrib- uted Testing: Models in The USA Using Jellett Rapid Tests 3:40 Reich, Andrew Development of Public Health Response Plans for HABS: a “County Up” Approach Presentation Schedule 5 3rd Symposium on Harmful Algae in the U.S. October 2005 Wednesday, October 5, 2005 Session 9: Bloom Ecology Time Presenter Title 8:40 Anderson, Don Intrapopulation Variation of Alexandrium fundyense within the Gulf of Maine: Ribosomal DNA and Microsatellite Analyses 9:00 Erdner, Deana L. Global Gene Expression Analysis of Nitrogen and Phosphorus Stress in the Toxic Dinoflagellate Alexandrium fundyense 9:20 Coyne Katherine J. Nitrate Assimilation in Heterosigma akashiwo: Evalua- tion of Nitrate Reductase (NR) Gene Expression in Laboratory Cultures and in Situ Populations of H. akashiwo in the Delaware Inland Bays, DE. 9:40 Parrow, Matthew W. Population DNA Distibution, Cellular DNA Content and the Diel NDA Cell Cycle of Cultured Karlodinium spp. (Dinophyceae) Break 10:00 am - 10:30 am Session 10: Bloom Ecology 10:30 Belas, Robert Motility of a Dinoflagellate-Associated Bacterium, Silcibacter sp. TM1040, is Important in its Interaction with Pfiesteria Piscicida 10:50 Glibert, Patricia M. Urea is a Good Predictor of Cyanobacteria in Florida Bay and on the Western Florida Shelf 11:20 Gobler, Christopher The Impact of Nutrient Loading and Zooplankton Grazing on the Growth of, and Toxin Synthesis by, cyanobacteria Blooms in Lake Agawam, NY, USA 11:30 Haas, Leanoard W. The Role of Dissolved Organic Matter in Heterotrophic Dinoflagellate Growth Lunch 12:00pm - 1:30 pm Session 11: Bloom Ecology 1:30 Twiner, Michael Comparative Brevetoxin Dynamics During Lysis of Karenia brevis by Two Algicidal Bacteria 1:50 Sengco, Mario R. Flow Effects on Interactions Between Karenia brevis and Clay Used in HAB Mitigation 2:10 Heil, Cynthia A. Nutrient Quality Drives Differential Phytoplankton Community Composition on the West Florida Shelf During a Karenia brevis Bloom Break 2:30pm - 3:00pm Session 12: Bloom Ecology 3:00 Schaeffer, Blake A Biochemical Investigation of Karenia brevis across a front off Sarasota, FL. 3:20 Anderson, Don Bloom Development and Transport of Toxic Alexandrium fundyense Populations Within a Coastal Plume in the Gulf of Maine 3:40 Hutchins, David A. Defining Ecological Niches Within a Multi-Species Raphidophyte HAB Consortium 6 Presentation Schedule October 2005 3rd Symposium on Harmful Algae in the U.S. Thursday, October 6, 2005 Session13: Bloom Ecology Time Presenter Title 8:40 Trainer, Vera L. Characteristics of the Juan de Fuca Eddy, a Source of Domoic Acid to the Washington Coast 9:00 Hickey, Barbara M. A Lagrangian View of the Juan de Fuca Eddy: Macro- nutrients and Circulation 9:20 Lessard, Evelyn J. Ups and Downs in the Life of a Toxic Pseudo-nitzschia Bloom in the Juan de Fuca Eddy off the Washington Coast 9:40 MacFadyen, Amy Circulation and Biological Modeling in the ECOHAB PNW Region Break 10:00 am - 10:30 am Session 14: Bloom Ecology 10:30 Edwards, Kathleen A. A Satellite View of Spatial and Temporal Variability of Chlorophyll a and SST in Coastal PNW Waters 10:50 Boyer, Gregory MERHAB - Lower Great Lakes -Monitoring for Harmful Algal Blooms in Our Inland Seas 11:20 Mayali, Xavier Ecdysis as a Defense Mechanism Against Bacterial Colonization: The Case of the Dinoflagellate Lingulodinium polyedrum 11:30 Lakeman, Michael B. Towards Recognition of Cryptic Functional Diversity in Natural HAB Populations Lunch 12:00pm - 1:30 pm Session 15: Bloom Ecology 1:30 Tester, Patricia A. Dinoflagellate Abundance in Mangrove Cay Embayments off the Coast of Belize 1:50 Parsons, Michael L. The Autecology of Gambierdiscus in the coastal waters of Hawaii 2:10 Tomas, Carmelo Growth, Nutrient Utilization and Evidence for Toxin Production by the New Toxic Flagellate Chloromorum toxicum. Break 2:30pm - 3:00pm Session 16: Bloom Ecology 3:00 Smayda, Ted Multidecadal Changes in the Diatom:Flagellate Ratio and Si:N and Si:P Ratios in Narragansett Bay, and Influence of Si:N Supply Ratios on Diatom Species Competition 3:20 Dortch, Quay Business meeting 3:40 TBD Site selection for next meeting Presentation Schedule 7 3rd Symposium on Harmful Algae in the U.S. October 2005 Public Outreach and Infrastructure – Posters PO1 Dalpra, Dana R. Are All Those Outreach Materials We’re Creating Doing Any Good? PO2 Fisher, Kathleen, M. Assessment of an operational Harmful Algal Bloom Forecast System for the Gulf of Mexico PO3 Greenfield, Dianne I. Application of the Environmental Sample Processor (ESP) for Remote Detection of Harmful Algae PO4 Petrik, Kim Molecular Detection of Karenia brevis and Related Species Using Fluorescent in situ Hybridization Assays PO5 Pigg, Ryan MERHAB 2002: Eastern GOMx Sentinel Program PO6 Poulton, Nicole J. Detection and Enumeration of Harmful Algal Bloom Species Using a Continuous Imaging Fluid Particle Analyzer (FlowCAM) PO7 Sinigalliano, Christopher Isolation of Toxic Algae from Marine Waters by High-Speed Flow Cytometric Single-Cell Sorting Toxins – Posters T1 Abbott, Jay P. Statewide Distribution of Saxitoxins in Selected Florida Puffer Fish Species (withdrawn) T2 Adolf, Jason Ichthyotoxic Karlocinium micrum in the Swan River Estuary (Western Australia): An Emerging Threat in a Highly Eutrophic Estuarine System T3 Bai, Xuemei Effect of Host Toxicity on Success of the Parasitic Di- noflagellate Amoebophrya, with Preliminary Examination of Host and Parasite Membrane Sterol Composition T4 Baugh, Keri Stability of Domoic Acid Under Various Storage Conditions T5 Bill, Brian D. Domoic Acid in Pseudo-nitzschia cuspidata from Washington State Coastal Waters T6 Bottein, Marie-Yasmine Detection of Ciguatoxin in the Blood of Patients Diagnosed with Ciguatera Intoxication T7 Bouillon, Rene-Christian Photochemistry of Dissolved Domoic Acid in Natural Water Matrices T8 Eberhart, Bich-Thuy Application of a Polychlonal Antibody in the Development of Methods for Detecting Domoic Acid T9 Faust, Maria A. The Biodiversity of Harmful Dinoflagellates in the Belizean Coral Reef Mangrove Forest T10 Ferry, John Applying Combinatorial Photochemistry Techniques to Explore the Photodegratation of the Harmful Algal Bloom Toxin Domoic Acid 8 Presentation Schedule October 2005 3rd Symposium on Harmful Algae in the U.S. T11 Dyer, Brian Susceptibility of Two Fishes (O. Niloticus and C. Variegatus) to Pfiesteria shumwayae and it’s Associated Toxin: Influ- ence of Salinity T12 Grover, James P. Growth, Toxicity and Composition of Prymnesium parvum in Relation to Temperature, Light and Salinity T13 Henry, Michael S. Aerosolized Brevetoxins: Compositional Changes from Water Bourne Brevetoxins to Aerosolized Brevetoxins Im- pacting Human Respiration T14 Hotto, Amber, M. Potential and Actual Microcystin Production in Lake Ontario Embayments T15 Keltner, Karen Pseudo-nitzschia australis: The Most Abundant Species of the Genus Pseudo-nitzschia at Two Central California Sites South and North of Pt. Conception, 2003-2005 T16 Litaker, R. Wayne Development of a Simplified ELISA for Detecting Domoic Acid T17 Lovko, Vincent J. Factors Regulating Micropredation and Fish Pathogenicity in Heterotrophic “Pfiesteria-Like” Dinoflagellates T18 Fahnenstiel, Gary Assessment of Microcystins Using Surface-Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry T19 Neely, Tatum A Modified Hemolytic Assay Suggests Toxin Activity Among Karenia mikimotoi Clones Isolated During Red Tide Events off the Texas Coast T20 Pierce, Richard Brevetoxins and Metabolites in NSP-Toxic Bivalve Molluscs: A Comparison of Methods T21 Satchwell, Mike Cyanobacterial Toxins in Lake Champlain - a Five Year Review T22 Schnetzer, Astrid Pseudo-nitzschia spp. and Domoic Acid in the San Pedro Channel and Los Angeles Harbor Areas of the Southern California Bight T23 Silver, Mary Toxic Pseudo-nitzschia from California: Some Emerging Patterns T24 Smith, G. Jason Molecular Physiology of Amino Acid Metabolism in Pseudo- nitzschia australis: Biomarkers for Growth Status or Domoic Acid Toxicity? T25 Sutherland, Cristy M. Dinophysis abundance, DSP Toxin Production, & Bioaccumulation in California Mussels, Mytilus Californianus, in Monterey Bay, CA, USA T26 Terlizzi, Daniel E. Membrane Sterols and Inhibition of Dinoflagellates by Karlodinium micrum Filtrate T27 Wang, Zhihong Analysis of Brevetoxin Metabolites in Bottlenose Dolphins Associated with Their Mortality in the Florida Panhandle During 2004 Presentation Schedule 9 3rd Symposium on Harmful Algae in the U.S. October 2005 T28 Westrick, Judy Preliminary Occurrence Study of Algal Toxin in Source and Finished Waters T29 Wright, Jeffrey L.C. Structure of Several New Hemolytic Toxins from Strains of Prymnesium parvum Isolated from Fish Ponds in North Carolina Food Web – Posters FW1 Antrobus, Rozalin Oceanographic Conditions in Monterey Bay, CA as They Relate to Alexandrium catenella and PSP Toxins in Local Fisheries: A Two Year Time Series FW2 Atwood, Karen Brevetoxin Body Burdens in Seabirds from the Central West Florida Coast FW3 Bargu, Sibel An Overview of the Domoic Acid Contamination of Monterey Bay Food Webs FW4 Borkman, David Modification of Heterosigma akashiwo Annual Succession Patterns in Narragansett Bay: Influence of Long-Term (1959 - 1996) Habitat Changes on Interspecific Competition FW5 Bretz, Carrie K. Toxic Prey Can Alter Foraging Strategies of Key Marine Predators FW6 Busse, Lilian B. Did We Have Toxic Algal Blooms in California in the Past? - Some Insights from Historical Data FW7 Casper, Erica T. A Handheld Device for the Detection of Karenia Brevis via NASBA FW8 Cheung, Itchung Presence of Domoic Acid in California Rock Crabs, Cancer antennarius And Cancer productus in Monterey Bay, Cali- fornia FW9 Deeds, Johnathan Puffer Fish: An Emerging reservoir for Saxitoxins in Marine Food Webs in the US FW10 Gobler, Christopher Investigating the Role of Zooplankton Grazing in Control- ling Harmful Brown Tide Blooms (Aureococcus anophagefferens) in Mid-Atlantic Estuaries FW11 Doucette, Gregory Exposure of North Atlantic Right Whales to Algal Biotoxins: The Proof is in the Poop FW12 Postel, J.R. Copepods and Diatoms: Paradigm or Paradox? FW13 Gribble, Kristin E Asexual and Sexual Reproduction in the Heterotrophic Dinoflagellate Protoperidinium oblongum FW14 Bricelj, V. Monica Transfer of Brevetoxins to The Benthos in the Context of Clay Mitigation of Karenia brevis Blooms FW15 Hégaret, Hélène No Apparent Effect of Two Species of the Toxic Dinoflagel- late Alexandrium on Hemocyte Parameters of the Oysters Crassostrea virginica and crassostrea gigas (withdrawn) 10 Presentation Schedule