Accepted Manuscript Title: Development of a one-step RT-PCR assay for detection of pancoronaviruses (�-, �-, �-, and �-coronaviruses) using newly designed degenerate primers for porcine and avian fecal samples Authors: Hui Hu, Kwonil Jung, Qiuhong Wang, Linda J. Saif, Anastasia N. Vlasova PII: S0166-0934(17)30492-5 DOI: https://doi.org/10.1016/j.jviromet.2018.02.021 Reference: VIRMET 13436 To appear in: Journal of Virological Methods Received date: 31-7-2017 Revised date: 18-1-2018 Accepted date: 26-2-2018 Please cite this article as: Hu H, Jung K, Wang Q, Saif LJ, Vlasova AN, Development of a one-step RT-PCR assay for detection of pancoronaviruses (�-, �-, �-, and �-coronaviruses) using newly designed degenerate primers for porcine and avian fecal samples, Journal of Virological Methods (2010), https://doi.org/10.1016/j.jviromet.2018.02.021 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 1 Journal of Virological Methods (Short communication) Development of a one-step RT-PCR assay for detection of pancoronaviruses (α-, β-, γ-, and δ-coronaviruses) using newly designed degenerate primers for porcine and avian fecal samples Hui Hu1, 2, Kwonil Jung1, Qiuhong Wang1, Linda J. Saif 1*, Anastasia N. Vlasova1* 1 Food Animal Health Research Program, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, 44691 United States 2 College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou, 450002 China * Co-corresponding authors: Anastasia Vlasova (