Volume 195 1991 Table of contents No. 1 1—288 issued on January 15, 1991 No.2 289—584 issued on January 30, 1991 No.3 585—878 issued on February 14, 1991 Acknowledgements to Referees 1-7 Specific valylation identity of turnip yellow mosaic virus RNA by yeast valyl-tRNA synthe- Corrections 287, 871 tase is directed by the anticodon in a kinetic Information for Authors 873-878 rather than affinity-based discrimination C. Florentz, T. W. Dreher, J. Rudinger and R. Giege 229-234 Molecular cloning and chromosomal localiza- tion of a human peripheral-type benzodiaze- Reviews pine receptor ‘Crosstalk’: a pivotal role for protein kinase C J. Riond, M. G. Mattei, M. Kaghad, in modulating relationships between signal X. Dumont, J. C. Guillemot, G. Le Fur, transduction pathways D. Caput and P. Ferrara 305-311 M. D. Houslay inhibition of protein synthesis by antagonists Sensory transduction in eukaryotes — A com- of calmodulin in Ehrlich ascites tumor cells parison between Dictyostelium and vertebrate R. V. Kumar, R. Panniers, A. Wolfman and cells E. C. Henshaw 313-319 P. J. M. Van Haastert, P. M. W. Janssens Gene for the ADP-ribosylatable elongation and C. Erneux 289-303 factor 2 from the extreme thermoacidophilic Regulation of the expression of the sex-spe- archaebacterium Sulfolobus acidocaldarius — cific isoforms of cytochrome P-450 in rat liver Cloning, sequencing, comparative analysis V. Kobliakov, N. Popova and L. Rossi 585-591 J. Schroder and F. Klink 321-327 Structures and characterization of sex-specific mouse cytochrome P-450 genes as members within a large family — Duplication boundary and evolution Nucleic acids, protein synthesis M. Lakso, R. Masaki, M. Noshiro and and molecular genetics M. Negishi 477-486 NMR studies of bipyrimidine cyclobutane Multiple «-tubulin isoforms in cilia and cyto- photodimers skeleton of Tetrahymena pyriformis generated T. M. G. Koning, J. J. G. van Soest and by post-translational modifications — Studies R. Kaptein during reciliation Activation of the double-stranded RNA-de- D. Penque, L. Galego and C. Rodrigues- pendent elF-2a kinase by cellular RNA from Pousada 487-494 3T3-F442A cells Structures of regulatory regions in the human J. Li and R. A. Petryshyn 41- 48 cytochrome P-450,... (desmolase) gene Simian-virus-40 large-T-antigen-catalyzed H. Inoue, N. Watanabe, Y. Higashi and DNA and RNA unwinding reactions Y. Fujii-Kuriyama 563-569 M. Scheffner, R. Knippers and H. Stahl 49- 54 Highly conserved structure of proliferating cell Evolutionary analysis of the nucleus-encoded nuclear antigen (DNA polymerase 6 auxiliary subunits of mammalian cytochrome c oxidase protein) gene in plants C. Saccone, G. Pesole and B. Kadenbach 151-156 |. Suzuka, S. Hata, M. Matsuoka, S. Kosugi and J. Hashimoto 571-575 Reverse transcriptase in archaebacteria — Pu- rification and characterization of a primase— Genomic organization of the human reverse-transcriptase complex procollagen «1 (Il) collagen gene from Halobacterium halobium M.-C. Huang, J. M. Seyer, J. P.T hompson, K. Ben-Mahrez, |. Sorokine, M. Nakayama D. G. Spinella, K. S. E. Cheah and and M. Kohiyama 157-162 A. H. Kang Linker mutagenesis in the /acZ gene of Esche- Secondary structure of the 5’ external richia coli yields variants of active f-galactosi- transcribed spacer of vertebrate pre-rRNA — dase Presence of phylogenetically conserved A. Breul, W. Kuchinke, B. von Wilcken- features Bergmann and B. Muller-Hill 191-194 B. Michot and J.-P. Bachellerie 601-609 The major serum protein of Drosophila larvae, ADP is a substrate for the AAUAAA-directed larval serum protein 1, is dispensable poly(A) addition reaction catalyzed by HeLa D. B. Roberts, T. Jowett, J. Hughes, cell nuclear extracts D. F. Smith and D. M. Glover 195-201 J. Lakota and B. D. Nelson 685-689 IV Table of contents The substrate specificity of protein kinases Cuttlefish sperm protamines — 2. Mass which phosphorylate the « subunit of spectrometry of protamines and related eukaryotic initiation factor 2 peptides C. G. Proud, D. R. Colthurst, S. Ferrari and P. Schindler, F. Bitsch, K. Klarskov, L.A. Pinna 771-779 P. Roepstorff, G. Briand, D. Wouters- An additional promoter functions in the human Tyrou, P. Sautiére and A. Van Dorsselaer 621-629 aldolase A gene, but not in rat Two structural domains as a general fold of the T. Mukai, Y. Arai, H. Yatsuki, K. Joh and toxic fragment of the Bacillus thuringiensis K. Hori 781-787 6-endotoxins D. Convents, M. Cherlet, J.V an Damme, |. Lasters and M. Lauwereys 631-635 Thrombin cleavage analysis of a novel antihaemophilic factor variant, factor VIII A Il S. Krishnan, H. V. J. Kolbe, P. Lepage, Protein chemistry and structure T. Faure, R. Sauerwald, H. de la Salle, C. Mulier, N. Bihoreau, P. Paolantonacci, C. Roitsch, P. Meulien and A. Pavirani 637-644 The amphiphilic «-helix concept — Conse- quences on the structure of staphylococcal Tryptophan phosphorescence of G-actin and d-toxin in solution and bound to lipids F-actin E. Thiaudiére, O. Siffert, J.-C. Talbot, G. B. Strambini and S. S. Lehrer 645-651 J. Bolard, J. E. Alouf and J. Dufourcq 203-213 Efficient renaturation and fibrinolytic Construction and characterization of a func- properties of prourokinase and a deletion tional chimeric murine — human antibody di- mutant expressed in Escherichia coli as rected against human fibrin fragment-D dimer inclusion bodies F. Bulens, A.-M. Vandamme, H. Bernar, G. Orsini, A. Brandazza, P. Sarmientos, L. Nelles, R. H. Lijnen and D. Collen 235-242 A. Molinari, J. Lansen and G. Cauet 691-697 Amino acid and cDNA sequences of a methio- Blocking of the receptor-mediated invasion of nine-rich 2S protein from sunflower seed erythrocytes by Plasmodium knowlesi malaria (Helianthus annuus L.) with sulfated polysaccharides and glycos- A. A. Kortt, J. B. Caldwell, G. G. Lilley and aminoglycans T. J. Higgins 329-334 J. P. Dalton, D. Hudson, J. H. Adams and L. H. Miller 789-794 Conformational change of turkey-gizzard cal- desmon induced by specific chemical modifi- Characterization of the cell-cycle-regulated cation with carbodiimide protein calcyclin from Ehrlich ascites tumor F. Martin, M.-C. Harricane, E. Audemard, cells — Identification of two binding proteins F. Pons and D. Mornet 335-342 obtained by Ca?* -dependent affinity chromatography An unusual class | (Schiff base) fructose-1,6- A. Filipek, V. Gerke, K. Weber and bisphosphate aldolase from the halophilic ar- J. Kuznicki chaebacterium Haloarcula vallismortis G. Krishnan and W. Altekar 343-350 Characterization of the cellular binding domain and the effects of monoclonal antibodies and Isolation and structure elucidation of a novel thrombin inhibitors on the binding and adipokinetic hormone (Lom-AKH-IIl) from internalization of the antithrombin-III— the glandular lobes of the corpus cardiacum of thrombin complex by cultured cells the migratory locust, Locusta migratoria S. Knoller and N. Savion 801-806 R. C. H. M. Oudejans, F. P. Kooiman, W. Heerma, C. Versluis, A. J. Slotboom and Three-dimensional correlated NMR study of A. M. T. Beenakkers 351-359 Megasphaera elsdenii flavodoxin in the oxidized state cDNA sequence and deduced amino acid se- S. S. Wijmenga and C. P. M. Mierlo 807-822 quence of the precursor of the 37-kDa inner envelope membrane polypeptide from spinach chloroplasts — Its transit peptide contains an Enzymology amphiphilic «-helix as the only detectable structural element Thymidine kinase from Tetrahymena thermo- U. Dreses-Werringloer, K. Fischer, phila — Purification and immunological analy- E. Wachter, T. A. Link and U.-1. Flugge 361 -368 sis P. W. Kinyanjui and R. E. Pearlman Determination of the covalent structure of an N- and C-terminally blocked glycoprotein Purification of a cysteine endopeptidase which from endocuticle of Locusta migratoria — is secreted with bioactive peptides from the Combined use of plasma desorption mass epidermal glands of Xenopus laevis spectrometry and Edman degradation to study N. J. Darby, D. B. Lackey and D. G. Smyth post-translationally modified proteins Submicromolar manganese dependence of G. Talbo, P. Hojrup, H. Rahbek- Nielsen, Golgi vesicular galactosyltransferase (lactose S. O. Andersen and P. Roepstorff 495-504 synthetase) Cuttlefish sperm protamines — 1. Amino acid N. J. Kuhn, S. Ward and W. S. Leong 243-250 sequences of two distinct variants Mechanism of the inhibition of «-thrombin by A. Martin-Ponthieu, D. Wouters-Tyrou, hirudin-derived fragments hirudin(1 — 47) and D. Bélaiche, P. Sautiére, P. Schindler and hirudin(45 — 65) A. Van Dorsselaer 611-619 T. Schmitz, M. Rothe and J. Dodt 251-256 Table of contents Purification and properties of an aryl-alcohol Investigations on microbial sulfur dehydrogenase from the white-rot fungus respiration — Isolation, purification, and Phanerochaete chrysosporium characterization of cellular components from A. Muheim, R. Waldner, D. Sanglard, Spirillum 5175 J. Reiser, H. E. Schoemaker and A. Zophel, M. C. Kennedy, H. Beinert and M. S. A. Leisola 369-375 P. M. H. Kroneck 849-856 Kinetic characterization of the enzymatic acti- vity of the eEF-2-specific Ca?* - and calmodu- Carbohydrates, lipids and other natural products lin-dependent proteinkinase II! purified from rabbit reticulocytes NMR investigations of the N-linked oligosac- A. Nilsson, U. Carlberg and O. Nygard 377-383 charides at individual glycosylation sites of hu- Paramagnetic centers and acetyl-coenzyme A/ man lutropin CO exchange activity of carbon monoxide de- G. Weisshaar, J. Hiyama, A. G. C. Renwick hydrogenase from Methanothrix soehngenii and M. Nimtz 257-268 M. S. M. Jetten, W. R. Hagen, A. J. Pierik, Rapid characterization of asparagine-linked A. J. M. Stams and A. J. B. Zehnder 385-391 oligosaccharides isolated from glycoproteins Fine tuning of the catalytic properties of hu- using a carbohydrate analyzer man carbonic anhydrase || — Effects of varying K. R. Anumula and P. B. Taylor 269-280 active-site residue 200 Carbohydrate microheterogeneity of rat sero- G. Behravan, B.-H- Jonsson and transferrin — Determination of glycan primary S. Lindskog 393-396 structures and characterization of a new type Demonstration of a molybdenum- and of trisialylated diantennary glycan vanadium-independent nitrogenase in a G. Spik, B. Coddeville, G. Strecker, nifHDK-deletion mutant of Rhodobacter J. Montreuil, E. Regoeczi, P. A. Chindemi capsulatus and J. R. Rudolph 397-4C5 K. Schneider, A. Muller, U. Schramm and Covalent binding of dolichy! phosphate to W. Klipp 653-661 proteins in rat liver Why do many Michaelian enzymes exhibit an A. Thelin, P. Low, T. Chojnacki and equilibrium constant close to unity for the G. Daliner interconversion of enzyme-bound substrate and product? G. Pettersson 663-670 Physical and inorganic biochemistry Kinetics of the conformational transition of the S = 9/2 EPR signals are evidence against cou- spinach chloroplast fructose-1,6-bis- pling between the siroheme and the Fe/S clus- phosphatase induced by fructose 2,6-bis- ter prosthetic groups in Desulfovibrio vulgaris phosphate (Hildenborough) dissimilatory sulfite reduc- J.-M. Soulié, M. Riviére, P. Baldet and tase J. Ricard 671-678 A. J. Pierik and W. R. Hagen 505-516 Synthesis of myristoyl-carba(dethia) - coenzyme A and S-(3-oxohexadecy)) - Membranes and bioenergetics coenzyme A, two potent inhibitors of myristoyl-CoA: protein N-myristoyl- Mechanism and energetics of a citrate-trans- port system of Klebsiella pneumoniae transferase A. P. Wagner and J. Rétey 699-705 M. E. van der Rest, T. Abee, D. Molenaar and W. N. Konings Dependence of lysozyme-catalysed On the mechanism of sodium ion translocation solubilization of Proteus mirabilis by methylmalonyl-CoA decarboxylase from peptidoglycan on the extent of O-acetylation C. Dupont and A. J. Clarke 763-769 Veillonella alcalescens W. Hilpert and P. Dimroth An acryl-carrier- protein —thioesterase domain Analysis of inhibitor binding to the mitochon- from the 6-deoxyerythronolide B synthase of drial cytochrome c reductase by fluorescence Saccharopolyspora erythraea — High-level quench titration — Evidence for a ‘catalytic production, purification and characterisation in Escherichia coli switch’ at the Q, center U. Brandt and G. von Jagow 163-170 P. Caffrey, B. Green, L. C. Packrnan, B. J. Rawlings, J. Staunton and PF. Leadlay 823-830 Phosphate binding and ATP-binding sites Transcription of synthetic DNA containing coexist in Na*/K* -transporting ATPase, as sequences with dyad symmetry by wheat- demonstrated by the inactivating MgPO, germ RNA polymerase I! — Increased rates of complex analogue Co(NH3)4PO, product release in single-step addition E. Buxbaum and W. Schoner 407-419 reactions Secondary structure and orientation of the am- D. Job, C. Job, L. Mercoyrol and D. Shire 831-839 phipathic peptide GALA in lipid structures — An infrared-spectroscopic approach Lys631 residue in the active site of the E. Goormaghtigh, J. De Meutter, F. Szoka, bacteriophage T7 RNA polymerase -- Affinity V. Cabiaux, R. A. Parente and labeling and site-directed mutagenesis J.-M. Ruysschaert 421-429 T. G. Maksimova, A. A. Mustayev, E. F. Zaychikov, D. L. Lyakhov, Regulation of the proton/electron stoichiome- V. L. Tunitskaya, Kh. Akbarov, S. V. Luchin, try of mitochondrial ubiquinol: cytochrome c V. O. Rechinsky, B. K. Chernov and reductase by the membrane potential S. N. Kochetkov 841-847 G. Bechmann and H. Weiss 431-438 Vi Table of contents Purification, biosynthesis and cellular localiza- Signal transduction and molecular neurobiology tion of a major 125-kDa glycophosphatidyl- Isolation of schistosomin, a neuropeptide inositol-anchored membrane glycoprotein of which antagonizes gonadotropic hormones in Saccharomyces cerevisiae a freshwater snail C. Fankhauser and A. Conzelmann P. L. Hordijk, R. H. M. Ebberink, The Bacillus subtilis cytochrome-c oxidase — M. de Jong-Brink and J. Joosse 131-136 Variations on a conserved protein theme Monoclonal antibodies against various forms M. Saraste, T. Metso, T. Nakari, T. Jalli, of the adenylyl cyclase catalytic subunit and M. Lauraeus and J. van der Oost 517-525 associated proteins Structural and functional characteristics of S. Moliner, R. Simmoteit, D. Palm and polypeptide subunits of the bovine heart T. Pfeuffer 281-286 ubiquinol — cytochrome-c reductase complex A clonal rat pancreatic 6 cell line (Rin14B) T. Cocco, M. Lorusso, A.-M. Sardanelli, expresses a high number of galanin receptors M. Minuto, S. Ronchi, G. Tedeschi and negatively coupled to a pertussis-toxin-sensi- S. Papa tive cAMP-production pathway Isolation and inactivation of the nuclear gene B. Amiranoff, A. M. Lorinet and encoding the rotenone-insensitive internal M. Laburthe 459-463 NADH: ubiquinone oxidoreductase of Interaction of guanine-nucleotide-binding mitochondria from Saccharomyces cerevisiae regulatory proteins with chemotactic peptide C. A. M. Marres, S. de Vries and L. A. Grivell 857-862 receptors in differentiated human leukemic HL-60 cells M. Tohkin, N. Morishima, T. liri, K. Takahashi, M. Ui and T. Katada 527-533 Structural requirements for the binding of the pituitary adenylate-cyclase-activating peptide to receptors and adenylate-cyclase activation Metabolism and metabolic regulation in pancreatic and neuronal membranes Phosphorus-31 nuclear magnetic resonance P. Gourlet, M.-C. Woussen-Colle, of C6 glioma cells and rat astrocytes — Evi- P. Robberecht, P. de Neef, A. Cauvin, dence for a modification of the longitudinal M.-C. Vandermeers-Piret, A.V andermeers relaxation time of ATP and P; during glucose and J. Christophe 535-541 starvation Effects of phorbol ester and cholecystokinin |. Pianet, M. Merle, J. Labouesse and on the intracellular distribution of protein P. Canioni 87- 95 kinase C in rabbit pancreatic acini Metabolism of [2-'*C]succinate in renal cells A. G. H. Ederveen, S. E. van Emst-De Vries, determined by '°C NMR J. J. H. H. M. de Pont and A. W. H. Jans and R. Willem 97-101 P.H. G. M. Willems 679-683 Failure of channelling to maintain low concen- Linking phospholipase A, to phospholipid trations of metabolic intermediates turnover and prostaglandin synthesis in mast A. Cornish-Bowden 103-108 cell granules S. P. Chock, S. G. Rhee, L. C. Tang and Irreversible transitions in the 6-phospho- E. A. Schmauder- Chock 707-713 fructokinase/fructose 1,6-bisphosphatase Activation of a pertussis-toxin-sensitive cycle guanine-nucleotide-binding regulatory W. Schellenberger and J.-F. Hervagault 109-113 protein during desensitization of Substrate specificity of «2 +3-sialyltransfera- Dictyostelium discoideum cells to chemotactic ses in ganglioside biosynthesis of rat liver golgi signals H. Iber, G. van Echten and K. Sandhoff 115-120 B. E. Snaar-Jagalska, S. Van Es, F. Kesbeke Hepatocyte heterogeneity in uptake and meta- and P. J. M. Van Haastert bolism of malate and related dicarboxylates in Phosphatidylinositol-specific phospholipase perfused rat liver C induces biosynthesis of acetylcholinesterase B. Stoll and D. Haussinger 121-129 via diacylglycerol in Schistosoma mansonii The human dioxin-inducible NAD(P)H: qui- B. Espinoza, |. Silman, R. Arnon and none oxidoreductase cDNA-encoded protein R. Tarrab-Hazdai 863-870 expressed in COS-1 cells is identical to diaphorase 4 P. M. Shaw, A. Reiss, M. Adesnik, D. W. Nebert, J. Schembri and A. K. Jaiswal 171-176 Molecular cell biology Control of hepatic mitochondrial 3-hydroxy- 3-methyiglutaryl-CoA synthase during the Fe3* -transferrin and Fe3 * -asialotransferrin foetal/neonatal transition, suckling and deliver iron to hepatocytes by an identical weaning in the rat mechanism P. A. Quant, D. Robin, P. Robin, P. Ferre, R. J. Sharma and D. A. W. Grant 137-143 M. D. Brand and J. Girard 449-454 The vacuolar protein-targeting signal of yeast Oxygen tension does not affect urea synthesis carboxypeptidase is functional in oocytes from in perifused rat hepatocytes Xenopus laevis L. Boon and A. J. Meijer 455-457 T. Roitsch and L. Lehle 145-150 Table of contents Highly purified pea chloroplast RNA polyme- Identification of a novel nuclear protein rase transcribes both rRNA and mRNA genes synthesized in growth-arrested human V. K. Rajasekhar, E. Sun, R. Meeker, hepatoblastoma HepG2 cells B.-W. Wu and K. K. Tewari 215-228 G. Donadel, C. Garzelli, R. Frank and F. Gabrielli 723-729 Molecular cell biology A tetranectin-related protein is produced and deposited in extracellular matrix by human Tumour-necrosis-factor-mediated cytotoxi- embryonal fibroblasts city is correlated with phospholipase-A, acti- |. Clemmensen, L. R. Lund, L. Christensen vity, but not with arachidonic acid release and P. A. Andreasen 735-741 per se P. Suffys, R. Beyaert, D. De Valck, B. Vanhaesebroeck, F. Van Roy and Developmental biochemistry and immunology W. Fiers 465-475 The human leukocyte-adhesion ligand, inter- Caldesmon-induced polymerization of actin cellular-adhesion molecule 2 — Expression from profilactin and characterization of the protein B. Gatlazkiewicz, F. Buss, B. M. Jockusch C. G. Gahmberg, P. Nortamo, and R. Dabrowska D. Zimmermann and E. Rouslahti 177-182 Tissue-specific and time-coordinated hor- Endopeptidase variations among different life- mone regulation of plasminogen-activator-in- cycle stages of African trypanosomes hibitor type | and tissue-type plasminogen ac- Z. R. Mbawa, |. D. Gumm, W. R. Fish and tivator in the rat ovary during gonadotropin- J. D. Lonsdale-Eccles 183-190 induced ovulation Comparison of myosin isoenzymes present in Y.-X. Liu, X.-R. Peng and T. Ny skeletal and cardiac muscles of the Arctic charr Requirement of down-regulation of NAD* Salvelinus alpinus (L.) — Sequential ADP-ribosyltransferase for the interferon-y- expression of different myosin heavy chains induced activation process of murine macro- during development of the fast white skeletal phage tumor cells muscle T. Taniguchi, S. Takahashi, H. Yamamoto, |. Martinez, J. S. Christiansen, R. Ofstad S. Fujimoto and H. Okoyama 557-562 and R. L. Olsen 743-753 Myosin heavy-chain-based isomyosins in de- veloping, adult fast-twitch and slow-twitch muscles A. Termin and D. Pette Indexed in Current Contents