California Amphibian and Reptile Crossing Preliminary Investigation Requested by Amy Bailey, Caltrans Division of Environmental Planning Prepared by Kendra K. Levine, Institute of Transportation Studies Library at UC Berkeley July 17, 2013 Executive Summary Background The efficacy of highway crossings for herptiles (the term that encompasses both reptiles and amphibians) is not as well documented as the efficacy of highway crossings for other species, such as mammals and fish. This is due, in part, to the lack of documentation and reliable data on herptile crossings. In 2012, a Preliminary Investigation was conducted entitled Highway Crossings for Herptiles (Reptiles and Amphibians).1 It summarized the key issues relevant to the design and best practices of herptile crossings. This Preliminary Investigation builds upon that report, focusing on 18 herptile species of particular interest in California. The effectiveness of a herptile crossing depends on the life histories and environment of the animals who will use it. Crossings should be only one component of mitigation efforts designed to minimize the effects of the “Road Zone” -- the altered area in and around roadways. Research has shown that animal reproduction is affected, not only by isolation, but by the changed environment created by the “Road Zone.” Ditches, culverts, and tire grooves can create unsuitable breeding grounds that stifle offspring yield.2 Summary of Findings Detailed habitat documentation and life histories are available for some of California’s endangered and threatened herptile species. This investigation begins the process of grouping potential crossing strategies, based on species life histories. But to conclusively measure the efficacy of a crossing treatment for a given population, actual experimentation and data collection is necessary. Researchers from New York State conducted such tests for the state’s 67 herptile species, and used the resulting data to develop models to identify potential crossing “hot spots” -- places 1 Highway Crossings for Herptiles (Reptiles and Amphibians); Caltrans Preliminary Investigation; November 2012; http://www.dot.ca.gov/newtech/researchreports/preliminary_investigations/docs/herptile_highway_crossings_pi201 2-11-2.pdf 2 “Ecological Effects of Roads Infrastructure on Herpetofauna: Understanding Biology and Increasing Communication”; Andrews, K.M. and Jochimsen, D.M.; Recent Work, Road Ecology Center, John Muir Institute of the Environment, UC Davis; May 2007 2 http://escholarship.org/uc/item/8d73q0mj 1 herptiles were most likely to use as a crossing.3 The team focused on minimizing road mortality at crossings. (While crossing location can play role, it was beyond the scope of the study.) While the effect of road mortality can significantly impact a species population, isolation is a larger concern for roads that bisect local herptile populations. Landscape fragmentation occurs when roads separate a group from its breeding grounds, hibernation locations, or adult habitats.4 The roads themselves may not directly cause mortality, but the disruption in habitat has an impact on the ability of populations to survive and reproduce. Herptile species often utilize general crossing treatments, such as viaducts or overpasses intended for mammals and other species. Amphibian/reptile tunnels have been recommended in locations where relatively high volumes of herptile crossings and a specific need already exist. Recommendations for herptile crossings from FHWA’s Handbook for Design and Evaluation of Wildlife Crossing Structures in North America5, and work from the Idaho Department of Fish and Game6 describe the key characteristics of crossing structures. While every crossing is context sensitive, it is possible to make generalizations for herptiles specific to their unique locomotion modes. Treatment Features/Reasons Tunnel Allows periodic migration for breeding without crossing a roadway. Natural substrate is often critical for herptiles, making it more likely they would use the tunnel (as opposed to bare concrete or metal). Wider tunnels allow for more natural light and airflow. Stream Crossing Amphibians often need water and moisture to successfully cross, just as in the natural environment. Drift Fence Acts as a barrier to prevent critters from crossing a roadway. Guides them to the designated crossing structure. Natural Light Some herptiles cross during the day or by moonlight; natural light is an important factor of their migration. Wide tunnels that allow some natural light and grating overhead are critical to maintain that environment. Area Closure Closes the area from use by humans to allow herptiles to migrate seasonally without threat from people. 3 Effects on New York State Roadways on Amphibians and Reptiles: A Research and Adaptive Mitigation Project; Gibbs, J.P et al; State University of New York College of Environmental Science and Forestry Department of Environmental and Forest Biology; March 2011; http://www.utrc2.org/sites/default/files/pubs/effects-nys-roadways- reptiles-final_0.pdf 4 “Effects of Habitat Fragmentation and Road Density on the Distribution Pattern of the Moor Frog Rana arvalis”; Vos, C.C. and Chardon, J.P.; Journal of Applied Ecology; no. 35, 1998; http://www.jstor.org/stable/2405186 5 Wildlife Crossing Structure Handbook: Design and Evaluation in North America; Clevenger, A.P. and Huijser, M.P.; FHWA; March 2011; 5http://www.cflhd.gov/programs/techdevelopment/wildlife/documents/01_Wildlife_Crossing_Structures_Handbook. pdf 6 A Literature Review of the Effects of Roads on Amphibians and Reptiles and the Measures Used to Minimize Those Effects; Jochminsen, D.M. et al; Idaho Department of Fish and Game; 2004; http://fishandgame.idaho.gov/public/wildlife/collisionAmphibRep.pdf 2 The following matrix shows recommended crossing strategies for the 18 herptile species of interest. Potential strategies were identified from the Handbook for Design and Evaluation of Wildlife Crossing Structures in North America. Specific strategies recommended for each species are a result of research on the individual life histories and habitats of the herptiles of interest, based on information found in environmental documentation from the U.S. Fish and Wildlife Service’s Recovery Plans (via the Environmental Conservation Online System7), the California Department of Fish and Wildlife’s “Threatened and Endangered Species” portal,8 and life sciences research databases, such as BIOSIS and PLOS One. Crossing Strategies for California Herptile Species of Interest Species Tunnel Stream Crossing Drift Fence Natural Light Area Closure Arroyo Toad ● ● ● Yosemite Toad ● ● ● Western Spadefoot ● ● ● ● California Red-Legged Frog ◯ ● Southern Mountain Yellow-Legged Frog ● Sierra Nevada Yellow-Legged Frog ● Western Pond Turtle ● ● Southern Rubber Boa Rosy Boa California Tiger Salamander ● ● ● Santa Cruz Long-Toed Salamander ◯ ● Coast Range Newt ● ● ◯ Western Skink San Joaquin Coachwhip Alameda Whipsnake California Mountain Kingsnake Ring-Necked Snake ◯ Giant Garter Snake ◯ ● - Recommended/Optimum Solution ◯ - Possibly effective, if tailored for local environment 7 “Environmental Conservation Online System”; U.S. Fish and Wildlife Service; http://ecos.fws.gov/ 8 “Threatened and Endangered Species”; California Department of Fish and Wildlife http://www.dfg.ca.gov/wildlife/nongame/t_e_spp/ 3 The most recommended crossing strategy is tunnels or stream crossings with a natural substrate. Guide fencing is frequently recommended to complement these treatments and ensure that the tunnels and stream crossings are used by the herptiles. Fencing must be made of an opaque, smooth fabric, constructed at a minimum height of 1.25 ft. so herptiles cannot climb over it.9 For some species of reptiles, paved roads can be attractive areas for basking. For others, paved roads disrupt the thermal environment and lead to population fragmentation. Some species, such as the Arroyo Toad10, find dirt roads enticing to cross11 or suitable for burrowing, Based on the survey of California herptiles of interest, it is apparent that roads and highways are just one type of development threatening herptile populations. Encroaching land development, such as housing subdivisions, and recreational areas, particularly those used for off-highway vehicles or camping, are also problematic. In addition, considerable damage has been done to herptile populations as a result of environmental pollution, including mercury contamination from abandoned mines.12 In these scenarios, the most direct mitigation strategy is to simply close down the area to human use during the migratory periods. For species, such as the Southern Mountain Yellow-Legged Frog and the Sierra Nevada Yellow- Legged Frog, the spread of disease within the population is an even greater threat to their survival than roads or subdivisions.13 Fungal infections and pesticides also adversely affect herptile populations. In addition, the threat of introduced fish and wildlife species that prey on or compete with native herptile species is another pressing issue. California Red-Legged Frogs are being threatened by introduced species, such as bullfrogs, trout, and crayfish, all of which are well suited to the habitat. These foreign species introduce new diseases to the local habitat.14 The introduction of the American Bullfrog (Rana catesbeiana) to the Sierra foothills has resulted in increased competition for food and resources with Yellow-Legged frogs, which are native to the area. American Bullfrogs are well suited to man-made environs, such as artificial streams15, and are therefore thriving in that habitat. 9 Wildlife Crossing Structure Handbook: Design and Evaluation in North America; Clevenger, A.P. and Huijser, M.P.; FHWA; March 2011; http://www.cflhd.gov/programs/techdevelopment/wildlife/documents/01_Wildlife_Crossing_Structures_Handbook. pdf 10 Arroyo Toad 5-Year Review: Summary and Evaluation; U.S. Fish and Wildlife Service, Ventura Fish and Wildlife Office; August 2009 http://www.biologicaldiversity.org/species/amphibians/arroyo_toad/pdfs/5_year_review_5-21- 10.pdf 11 Responses of Small Terrestrial Vertebrates to Roads in a Coastal Sage Scrub Ecosystem; Brehme, C.S.l San Diego State University; 2003; http://roadecology.ucdavis.edu/pdflib/TTP_289/W08/Roads_Thesis_CSB_Final.pdf 12 “Mercury contamination in three species of anuran amphibians from the Cache Creek Watershed, California, USA”; Hothem, R.L, Jennings, M.R. and Crayon, J.J.; Environmental Monitoring and Assessment; April 2010, v. 163, no. 1-4, pp 433-448; http://dx.doi.org/10.1007/s10661-009-0847-3 13 “Dynamics of an Emerging Disease Drive Large-Scale Amphibian Population Extinctions”; Vrendenburg, V.T. et al; PNAS, v.107 no.21, 2010; http://www.pnas.org/content/107/21/9689.short 14 http://www.epa.gov/espp/litstatus/effects/redleg-frog/attachment1.pdf 15 “Linking the Distribution of an Invasive Amphibian (Rana catesbeiana) to Habitat Conditions in a Managed River System in Northern California”; Fuller, T.E. et al; Restoration Ecology, v. 19, no. 201, pp 204–213, March 2011; http://dx.doi.org/10.1111/j.1526-100X.2010.00708.x 4 Gaps in Findings To date, very little research exists describing the direct impact of roads on the 18 California species of interest. While a handful of articles reference the current state of these herptile populations with some conjecture about the effects of roads and population fragmentation, they did not offer viable solutions or mitigation strategies. Not all the species examined are officially labeled as endangered, and therefore are not protected under existing U.S. Fish and Wildlife Service recovery plans. In addition, because very little information exists about the life histories and habitats of some of these herptile species, it is difficult to deduce the actual impact of human development upon them. While some of the examined species have such local and contained habitats, it might be relatively straightforward to minimize and mitigate impact upon them. Other species that have forage in wider ranges and therefore are more likely to come in contact with man-made development will require more consideration. Next Steps When implementing new herptile crossings, documenting and collecting data to assess the efficacy of the crossings is important. This data can be used to develop models to better predict crossing hot spots and future survival strategies. For species where little or no information is available that documents their life histories and habitats, collecting that data would be a good initial step. 5 Herptile Life Histories The 18 California herptile species of interest identified by Caltrans for this Preliminary Investigation are the following: Arroyo Toad Yosemite Toad Western Spadefoot Southern Mountain Yellow- (Anaxyrus californicus) (Anaxyrus canorus) (Spea hammondii) Legged Frog (Rana muscosa) Sierra Nevada Yellow- California Red-Legged Western Pond Turtle Western Skink (Plestiodon Legged Frog (Rana Frog (Rana draytonii) (Actinemys marmorata) skiltonianus) sierrae) Southern Rubber Boa Rosy Boa (Lichanura California Tiger Santa Cruz Long-toed (Charina umbratica) trivirgata) Salamander Salamander (Ambystoma (Ambystoma macrodactylum croceum) californiense) Coast Range Newt San Joaquin Alameda Whipsnake Ring-Necked Snake (Taricha torosa) Coachwhip (Masticophis lateralis (Diadophis punctatus) (Masticophis flagellum euryxanthus) ruddocki) California Mountain Giant Garter Snake Kingsnake (Thamnophis gigas) (Lampropeltis zonata) The life histories and habitats of the concerned species were collected to make grouping and classification of effective crossings more apparent. None of the species of interest have been formally studied with regard to the effect of road zones on their populations. For species designated as endangered or threatened by the U.S. Department of Fish and Wildlife, the recovery plan was examined to see if it included any recommendations related to roads or vehicles. Species still under review do not yet have recovery plans and are not as well documented as those currently classified as Not Listed. Documented road interactions are also mentioned, as well as any noted mitigation strategies. These life histories help inform possible crossing strategies for specific herptile populations. 6 Arroyo Toad (Anaxyrus californicus)16 Status Endangered (Federal) Special Concern (California) Location Los Angeles, Monterey, Orange County, Riverside, San Bernardino, San Diego, Santa Barbara, Ventura Counties. Road Interactions Threats from human development and off-road vehicles. Nighttime crossing and foraging on paved roads (particularly on rainy nights) leads to high mortality. Burrowing in dirt roads is a problem as they are crushed by traveling vehicles. Crossings/Mitigation Replacing culverts with stream crossings, adding roadside fences, and seasonal closing of campgrounds have led to greater breeding success. Breeding February to July in streams with persistent water. Slow moving, shallow streams and riparian habitats. Streams must be large enough for channel scouring. Eggs/Tadpoles/Metamorphosis Hatching occurs in 4-5 days. Substrate is sand or silt. Immobile for another 5-6 days, then disperse into shallow water for 10 weeks. Feed on loose organic material (algae). Young Adult Hangs out on the gravel on outsides until ponds dry out. Feeds on ants (and maybe beetles). Adult Feeds on insects and arthropods. Live in upland areas during non-breeding seasons; burrows in sand. 16 Arroyo Toad 5-Year Review: Summary and Evaluation; U.S. Fish and Wildlife Service, Ventura Fish and Wildlife Office; August 2009 http://www.biologicaldiversity.org/species/amphibians/arroyo_toad/pdfs/5_year_review_5-21- 10.pdf 16Recovery Plan for the Arroyo Southwestern Toad; U.S. Fish and Wildlife Service; 1999; http://ecos.fws.gov/docs/recovery_plan/990724.pdf 7 Yosemite Toad (Anaxyrus canorus)17 Status Proposed Threatened (Federal) Special Concern (California) Location El Dorado, Fresno, Inyo, Madera, Mono, Tulare Counties. Road Interactions No information. Crossings/Mitigation Culverts, stream crossings, and fencing in some areas. Breeding Mating is polygynous, mid-April to mid-July, depending on local conditions. Eggs laid in shallow, quiet pools, in wet meadows, or shallow tarns surrounded by forest. Eggs/Tadpoles/Metamorphosis Laid in shallow pools or tarns. Compete for space with Hyla regilla, Rana muscosa, and B. boreas. Feed on bottom detritus, suspended plant material, or planktonic animals. Adult Diurnal. Lives in moist microclimates. Migration can be extensive. Hibernation in meadow sod or vegetation. Quiet pools in alpine meadows is optimal habitat. Diet includes beetles, ants, mosquitoes, dragonfly nymphs, centipedes, and spiders. 17 “Yosemite Toad” A033; California Wildlife Habitat Relationships System, California Department of Fish and Wildlife Service; 2000; https://nrm.dfg.ca.gov/FileHandler.ashx?DocumentVersionID=18167 17Arroyo Toad (Anaxyrus californicus) Life History, Population Status, Population 17Threats, and Habitat Assessment of Conditions at Fort Hunter Liggett, Monterey 17County, California; Hancock, J.P.; California Polytechnic State University, San Luis Obispo; 2009; 17http://digitalcommons.calpoly.edu/cgi/viewcontent.cgi?article=1222&context=theses 17“Habitat Use by Yosemite Toads: Life History Traits and Implications for Conservation”; Morton, M.L. and Pereyra, M.E; Herpetological Conservation and Biology v. 5 no. 3; September 2010; 17http://www.herpconbio.org/Volume_5/Issue_3/Morton_Pereyra_2010.pdf 17“El Dorado County Integrated Natural Resources Management Plan-Phase I”; Sierra Ecosystem Associates; Wildlife Movement and Corridors Report; November 2010; https://www.edcgov.us/Government/Planning/INRMP/A_-_Final_Draft_Wildlife_Rpt.aspx 8 Western Spadefoot (Spea hammondii)18 Status Under Review (Federal) Special Concern (California) Location Throughout Central Valley, Coast Ranges, and coastal lowlands from San Francisco Bay toward Mexico. Road Interactions Sometimes they cross roads at night during migration periods. The balance between urban development and open spaces greatly affects them. Crossings/Mitigation Undercrossings and stream crossings that have natural substrate and ambient light. Fencing for guidance and as a barrier. Breeding Breed January to May in temporary pools, drainage from spring rains. Water must be between 48-86 degrees F. Oviposition does not occur until the water is 48 degrees F. Eggs are deposited on twigs or detritus in pools. Eggs/Tadpoles/Metamorphosis Eggs hatch in 0.6-6 days depending on the water temperature. Half the eggs often fail to develop, perhaps due to fungus in the water. Feed on planktonic animals and algae. Young Adult Once they leave the natal pool, their habits are similar to that of adults. Adult Feeds on variety of insects, worms, and invertebrates. Active nocturnally during period of rain or high humidity. Remains in burrows during much of the year, but surface during first rains. Movement is restricted, at most several meters on rainy nights. 18 “Western Spadefoot” A028; California Wildlife Habitat Relationships System, California Department of Fish and Wildlife Service; 2000; https://nrm.dfg.ca.gov/FileHandler.ashx?DocumentVersionID=18116 18Appendix O Road Design Guidelines; South Sacramento Habitat Conservation Plan; July 2010; 18http://www.per.saccounty.net/PlansandProjectsIn-Progress/Documents/SSHCPTOC/Appendix- O_Road%20Design%20Guidelines_final-mn.pdf 9 California Red-Legged Frog (Rana draytonii)19 Status Threatened (Federal) Special Concern (California) Location Found in the Coast Ranges from Mendocino County southward and in portions of the Sierra Nevada and Cascade Ranges. Road Interactions Found on roads at night during winter and spring rains, which results in a high rate of mortality. Population fragmentation is also disruptive. Crossings/Mitigation Undercrossings and stream crossing may be appropriate. Recovery plans largely focus on preserving breeding grounds through area closures during breeding and migration seasons. Breeding Eggs laid in permanent pools with emergent vegetation. Breeding occurs January to July in the south; March to July in the north. Eggs/Tadpoles/Metamorphosis Tadpoles need 11-20 weeks to reach metamorphosis, typically between May and September. Young Adult Juveniles feed day and night. Adult Active year round on the coast. More inland populations are inactive during fall and winter. Prefers shorelines with vegetation, and water with depths of 3ft. or more for escape. Primarily nocturnal. There is little movement from the streamside habitats. 19 “California Red-Legged Frog” A071; California Wildlife Habitat Relationships System, California Department of Fish and Wildlife Service; 2008 https://nrm.dfg.ca.gov/FileHandler.ashx?DocumentVersionID=17603 19“California Red-Legged Frog: Rana aurora draytonii”; Administrative Draft, Solano HCP; Solano County Water Agency; April 2009; http://www.scwa2.com/documents/hcp/Final%20Admin%20Draft/Appendix%20B/Inner%20Coast%20Range/Calif ornia%20Red-legged%20Frog.pdf 19“Status and Life History of California Red-Legged Frog”; Environmental Protection Agency; 2007 19http://www.epa.gov/espp/litstatus/effects/redleg-frog/attachment1.pdf 10