2003. The Journal of Arachnology 31:55-61 BOTHRIURUS JESUITA, A NEW SCORPION SPECIES FROM NORTHEASTERN ARGENTINA (SCORPIONES, BOTHRIURIDAE) Andres A. Ojanguren Affilastro: Museo Argentine de Ciencias Naturales “Bernardino Rivadavia,” Division Aracnologia, Av. Angel Gallardo 470, 01405 DJR Buenos Aires, Argentina. E-mail: [email protected] ABSTRACT. The new species Bothriurusjesuita is described. This species belongs to the bonariensis species group and is closely related to Bothriurus chacoensis Maury & Acosta 1993 from which it can be distinguished by its thicker and taller chela and because of it is almost 30% larger. It can be distin- guished from Bothriurus bonariensis (C.L. Koch 1842) because it has an apical filament on the basal lobe of the right hemispermatophore and because the frontal ridge reaches the frontal fold. Bothriurusjesuita has been collected in the northern region ofCorrientes Province and in Misiones Province in an area that belongs to the “Paranaense” Phytogeographic Province. RESUMEN. En el presente trabajo se describe a Bothriurusjesuita sp. nov., esta especie pertenece al grupo bonariensis y se encuentramuy relacionadaconBothriurus chacoensisMauryy Acosta 1993; puede diferenciarse de esta por poseer una pinza mas alta y robusta, y por ser casi un 30% mas grande. Puede diferenciarse de Bothriurus bonariensis (C.L. Koch 1842) por poseer un filamento en el lobulo basal del hemiespermatoforo derecho, y porque la cresta frontal de la lamina distal llega hasta el repliegue frontal. Bothriurusjesuita solo ha sido colectada en el norte de la provincia de Corrientes y en la provincia de Misiones, en un area correspondiente con la provincia fitogeografica Paranaense. Keywords^ Bothriurus, Neotropics, scorpion, taxonomy. The genus Bothriurus is the most diverse in new species: Bothriurus vianai (nomen nu- the family Bothriuridae. To facilitate identifi- dum, in schedula). Maury & Acosta (1993) cation of species in the genus, it was divided mention the presence ofthis new species from into species groups (Maury 1973, 1979, the Argentinian Mesopotamia, but they never 1984), one ofwhich is the bonariensis species attempted to describe it. group (Maury 1979). The distinctive charac- Bothriurusjesuita is the fourth species re- teristics ofthis group are as follows: chela tri- corded for the Misiones scorpiological area chobothrium esb forms a triangle with eb 2 (Acosta & Maury 1998), the other species and eb 3, hemispermatophore lamina very are: Tityus bahiensis (Perty 1833), Bothriu- well developed, straight with a frontal ridge, rus moojeni Mello Leitao 1945 and Anan- chelicerae with a single subdistal tooth, ven- teris balzanii Thorell 1891, although the trolateral and transverse carinae forming an presence of the last species in the area has arc in the distal third of the fifth metasomal to be confirmed (Mello Leitao 1945; Lou- segment, males metasomal gland placed in a ren9o 1993; Ojanguren Affilastro & Vezzani very conspicuous depression on the dorsal 2001). face of the telson (Maury & Acosta 1993; A map of the distribution of the species Acosta & Maury 1998). ofthe bonariensis group in Argentina is pro- Currently the bonariensis group contains vided. All the localities given in this map only two species: B. bonariensis (C.L. Koch belong to the bibliography cited in this pa- 1842) and B. chacoensis Maury & Acosta per, except for the new records ofBothriurus 1993. Bothriurusjesuita, a new species from jesuita. the bonariensis group, is herein described. METHODS This species was first recognized as distinct by Dr. San Martin; all the specimens exam- Terminology of the structures of the hemis- ined for this paper were labelled by him as a permatophore follows Maury (1973) andMaury 55 — —— 56 THE JOURNAL OF ARACHNOLOGY 368, 369, map (in part.); Maury 1979: 708, 715, fig. 5 (in part). Bothriurus sp: Maury & Acosta 1993: 114, 118. Type data. Holotype male, ARGENTI- NA: Misiones Province, Loreto (27°19'S, 55°3UW), 1936, Ogloblin coll. (MACN-Ar 10048). Paratypes: Misiones Province: San Ig- nacio (27°16'S, 55°31'W), 1 d, December 1948, Biraben coll. (MACN-Ar 10049); 1 d, December 1948, Biraben coll. (MACN-Ar 10052); San Javier (27°52'S, 55°7'W), 2 ju- veniles, December 1948, Biraben coll. (MACN-Ar 10050); Santo Pipd (27°7'S, 55°31'W), 1 d, 1 $, December 1951, Foenter coll. (MACN-Ar 10051); Santa Ana (27°22'S, 55°34'W), 1 d. Llamas coll. (MACN-Ar 10053); juvenile, Llamas coll. (MACN-Ar 1 10071); 1 d, March 1901, Llamas coll. (MACN-Ar 10057); San Juan (27°46'S, 55°30'W), 1 d, 3 August 1924, Gomez coll. (MACN-Ar 10054); Loreto (27°19'S, 55°3rW), 2juveniles, 15-20 December 1932, Figures 1-3. Bothriiirusjesiiita new species. 1. Ogloblin coll. (MACN-Ar 10055); 1 d, Feb- Right hemispermatophore, dorsal aspect; 2. Left he- ruary 1956, Sanchez de Bustamante coll. mispermatophore, dorsal aspect; 3. Bothnunis ho- (MACN-Ar 10063); d, 8 January 1933, 1 rAriibadbrgrieee;nvsiFiasFt,io=rnisgf:hrtoAnhtFaelm=ifsopladpe.ircmSaacltaohlpelhabomarernet,=;dFo1rRsmam=l.afsrpoenctta.l g((uMMa-AAiCC,NN2--5°AA5rr5'S11,0000556490°));3;6'1PWu)de,,rt11o0$B,Fee1bmrbjuuevarergnyil(e1,U9r32u75,- November 1948, (MACN-Ar 10056); San & Jose (27°46'S, 55°46'W), 3 juveniles, Decem- Acosta (1993). Trichobothrial terminology ber 1948, Biraben coll. (MACN-Ar 10058); fsoolnlgowlsanVdafcohlolnows(1M97a4u)r.yTe&rmAicnoosltoag(y19o9f3t)h.eTteerl-- Aniploes,toMlaersch(271°95335',S,Ca5s5t°el4l6i'Wc)o,ll.1 (dM,AC1 Nj-uvAer- minology of the Phytogeographic Provinces 10061); Pindapoy (San Jose, 27°46'S, & ftoaogly&loowfMsathuCearbSyrceo(rr1pa9i9o8l)o.WgiiTlcelarlimnAikrne(oa1l9so8gf0y)o.lolTfoewtsrhmeAicnmooesl--- rW5ii5al°l4(i26n7'e°Wr5)5,c'oSl,1l.5d(5,°M21A2jC'uWNv)e-,nAil3re,d1,010D27e0Jc)a;enmuSbaaernyrta1199M44a32-,, tasomal carinae follows Stahnke (1970). Viana coll. (MACN-Ar 10062); 1 d, Novem- MAbAbCreNv-iaAtrion=s fMoursecoolleAcrtgieonntsinaoredaes Cfioelnlcoiwas:s bde,r J1a9n5u2a,ryVia1n9a64c,ollV.ia(nMaACcNol-l.Ar(M1A00C6N4)-;Ar1 Naturales “Bernardino Rivadavia”, National 10065); 1 d, March 1945, Viana coll. Arachnological Collection (Cristina Scioscia). (MACN-Ar 10066); Id, December 1948, Via- All measurements are in mm, and were taken na coll. (MACN-Ar 10067). Province of Co- using an ocular micrometer. Illustrations were rrientes: Manantiales (27°56'S, 58°7'W), 1 d. produced using a stereomicroscope and cam- May 1947, Apostol coll. (MACN-Ar 10068); era lucida. The hemispermatophores were dis- Apipe (27°31'S, 56°43‘'W), 1 d, December isnec8te0d%fertohmansoulr.rounding tissues and observed 194E5t,ymHoalnokgey.collT.h(eMAsCpeNci-fAicr e1p0i0t6h9e)t. comes Bothriurusjesuita, new species from the Spanish name of the Jesuit catholic Figs. 1, 2, 5-10, 12-16 order. This species has been mainly collected Bothriurus vianai: San Martin (nomen nudum, in near the ruins of the missions that the Jesuit schedula). order constructed in the XVII and XVIII cen- Bothriurus honarieusis (C.L. Koch): Maury 1973: turies. — 1 OJANGUREN AFFILASTRO—NEW SCORPION FROM ARGENTINA 57 — Figures 4-1 . Bothriurusjesuita new species, 5. Leftchela, male, ventral aspect; 6. Leftchela, female, ventral aspect; 7: Right chela, male, dorsal aspect; 8. Left chela, female, prolateral aspect; 9. Right chela, male, retrolateral aspect; 10. Left chela, male, prolateral aspect. 4, 11: Bothriurus chacoensis, 4. Left chela, male, ventral aspect; 11. left chela, male, prolateral aspect. Scale bar = 1 mm. Diagnosis. Bothriurusjesuitacanbedistin- mispermatophores, but Bothriurus jesuita has guishedfromB. bonariensisby having an apical a wider and higher pedipalp chela (Figs. 4- = filament on the basal lobe of the right hemis- 11); chela length/chela width: 2.6-3.02 (n permatophore (Fig. 1), that is lacking in the left 25; mean = 2.74) in B. chacoensis males, one (Fig. 2), and by the frontal ridge reaching 3.12-3.36 {n = 25; mean = 3.24) in females; the frontal fold, whereas in B. bonariensis it 2.25-2.56 {n = 18; mean == 2.47) in B.jesuita does not (Fig. 3). Bothriurusjesuita is morpho= males, 2.89-3.01 (« = 3; mean == 2.93) in logically most similar to B. chacoensis; both females; chela iength/chela height: 1.84-2.22 species share the particular shape of their he- {n = 25; mean = 1.97) in B. chacoensis males. 58 THE JOURNAL OF ARACHNOLOGY — Figures 12-14. Bothriuriisjesuita new species, 12. Fifth caudal segment, ventral aspect; 13. Telson, male, lateral aspect; 14. Telson, female, lateral aspect. Scale bar = 1 mm. 2.33-2.52 (/? = 25; mean = 2.4) in females; sternites dark brown with some yellow spots; 1.68-1.8 (n = 18; mean = 1.76) in B. jesuita legs dark brown with some yellow spots in males, 2.15-2.24 {n = 3; mean = 2.21) in coxa and trochanter; telson dark brown except females. Although in the extremes ofvariation the metasomal gland in males that is light yel- the ratios may overlap, no specimens have low. been found where both ratios overlap (Fig. Morphology: Measurements of male holo- 15), so to be certain of the identification of type and a female paratype (MACN-Ar the species both ratios must be used. Bothriu- 10051) in table 1. Prosoma: carapace: tegu- rus bonariensis ratios are similar to those of ment finely granular, front border with a slight B.jesuita, but in some specimens they overlap notch, median ocular tubercle located in the with those of B. chacoensis (Fig. 15); B. bo- center of the carapace, eyes one diameter nariensis chela length/chela height ratio: apart, postocular furrow and lateral sulcus males 1.68-1.85 (/? = 20; mean = 1.76), fe- moderately deep; chelicerae with a single sub- males 1.80-1.98 {n = 20; mean = 1.96); distal tooth. Mesosoma: tergites I to VI finely length/width ratio: males 2.36-2.66 {n = 20; granular, VII finely granular with two postero- mean = 2.50), females 2.97-3.20 {n = 20; lateral carinae; sternites smooth. Metasoma: mean = 3.06). Bothriurus jesuita is approxi- segment I: dorsosubmedian carinarepresented mately 30% longer than B. chacoensis', B. je- only by 4 or 5 coarse granules in the second suita'. males 39-47 mm (n = 18; mean = half of the segment, dorsolateral carinae pres- 44.7), females 52-59 mm (« = 3; mean = ent only in the second part of the segment, 55.3); B. chacoensis'. males 28-35 mm {n = median lateral carinae present only in the dis- 25; mean = 31.5), females 30-36 mm {n — tal third of the segment; segment II and III: 25; mean = 33.8). Bothriurus jesuita has a dorsosubmedian and dorsolateral carinae like darker and more uniform color pattern than B. in segment I, median lateral carinarepresented chacoensis. — by some tiny granules at the end of the seg- Description. Coloration: Carapace, pedi- ment; segment IV: dorsosubmedian carina like palps, tergites and metasoma: base color segment I, dorsolateral and median lateral ca- dusky brown with moderately dense variegat- rinae absent; segment V: ventrolateral carinae ed darker pattern; chelicerae and pectines in the distal third, forming an arc with the brown-yellow with underlying dusky pattern; transverse carinae which surrounds some scat- 1 OJANGUREN AFFILASTRO—NEW SCORPION FROM ARGENTINA 59 — Table 1. Bothriurusjesuita, measurements (mm) and number of pectinal teeth of the holotype male and a female paratype. Male holotype Female paratype Total length 42.76 52.28 Prosoma, length 4.85 5.66 Prosoma, anterior width 3.31 4.53 Prosoma, posterior width 5.82 7.1 Mesosoma, total length 11.41 18.58 Metasoma, total length 26.5 28.04 Metasomal segment I, length 2.51 3.39 Metasomal segment I, width 3.96 4.28 Metasomal segment I, height 2.83 3.56 Metasomal segment II, length 3.10 3.56 Metasomal segment II, width 3.88 4.12 Metasomal segment II, height 2.99 3.47 Metasomal segment III, length 3.47 3.88 Metasomal segment III, width 3.88 4.04 Metasomal segment III, height 3.23 3.39 Metasomal segment IV, length 4.36 3.64 Metasomal segment IV, width 3.79 4.04 Metasomal segment IV, height 3.31 3.72 Metasomal segment V, length 6.06 6.71 Metasomal segment V, width 3.72 4.04 Metasomal segment V, height 2.99 3.39 Telson, length 7.00 6.86 Vesicle, length 4.12 5.17 Vesicle, width 2.99 3.39 Vesicle, height 2.18 2.51 Aculeus, length 2.18 1.69 Pedipalp, total length 14.54 17.69 Femur, length 3.56 4.20 Femur, width 1.69 1.86 Patella, length 3.79 4.20 Patella, width 1.62 1.94 Chela, length 7.19 9.29 Chela, width 2.91 2.75 Chela, height 4.12 3.79 Movable finger, length 3.79 4.44 Number of pectinal teeth 22-22 18-18 tered granules, ventromedian carina occupies group: neobothriotaxic major type C: femur the whole segment except a small area near with 3 trichobothria: 1 d, 1 i and 1 e; patella the front border (Fig. 12). Telsoe: tegument with 3 V trichobothria and 13 trichobothria on finely granular, vesicle dorsoventrally com- its external face: 3 et, 1 est, 2 em, 2 esb and pressed (Fig. 13), aculeus short, in females the 5 eb; chela with 12 trichobothria on its pro- vesicle is more globose and the aculeus is lateral face: 1 est, 2 et, 5 v, 1 esb and 3 eb; shorter (Fig. 14), in males the metasomal no intraspecific variation has been observed in gland is placed dorsally in a very conspicuous this character; chela trichobothrium esb forms pit. Pedipalps; femur granulated, ventrointer- a triangle with eb 2 and eb 3. Legs: smooth nal and dorsointernal carinae poorly devel- tegument; ventrosubmedian spiniform setae of oped, patella smooth, chela high and thick, the telotarsi: telotarsus I: 1-fl, telotarsus II: with a ventroexternal carina, apophysis of 24-2, telotarsi III & IV: 3+3; no intraspecific males scarcely developed, short fingers, tri- variation has been observed in this character. chobothrial pattern typical of the bonariensis Hemispermatophore: distal lamina very well — 60 THE JOURNAL OF ARACHNOLOGY length/heightofthe pedipalpchela — Figure 15. Length/height ratio ofthe pedipalpal chela, against length/width ratio ofthe pedipalp chela; males ofBothriurusjesuita (grey triangles), males ofBothriurus chacoensis (black circles), and males of Bothriurus bonariensis (white squares). developed, straight with a frontal ridge that & Maury 1998); B. bonariensis is found in reaches the frontal fold; there is an apical fil- the “Pampeana” Province, although it has ament on the basal lobe ofthe right hemisper- also been found in the “Espinal” (Maury matophore that is lacking in the left (Figs. 1 1973; Maury 1979, 1986; Acosta & Rosso de & 2). — Ferradas 1996; Acosta & Maury 1998); and Variation. Total length in males, 39-47 B. jesuita, the most restricted species, has mm {n = 18; mean = 44.7), in females 52- been found so far only in the “Paranaense” 59 mm (ii = 3; mean = 55.3). Number of Province. pectinal teeth, in males: 20-23 {n — 18; me- In Manantiales, in the northern region of dian = 21), and in the 3 females examined 18. Corrientes Province, several specimens ofB. = Chela length/chela width: 2.25—2.56 {n 18; chacoensis have been found, together with a mean = 2.47) in males, 2.89-3.01 {n = 3; specimen of B, jesuita. Since this locality hm1e.ei7ag6h)n,t:=2.11.256.-8923-.)12.48in{infne=mmaall3e;ess;me{canhne=la=1l28e;.n2g1mt)he/aicnnhefle=a- il“siCeshnoaatqtustethnreaanb”goerPdhteoyrtfooigfnedothgberoat“phPhaisrcpeacnPiraeoesvnisinenc”essy,amn-idt i males. I patry. It is very probable that B. jesuita is Distribution. Bothriurusjesuita has been I also present in southern Brazil and eastern collected in Argentina, in the northern part of Paraguay, although we have not had access Corrientes Province and in Misiones Province | to any specimen that confirms this distribu- (Fig. 16). This area belongs to the “Paranaen- , se’' Phytogeographic Province, and to the Mi- tion. siones Scorpiological Area. ACKNOWLEDGMENTS : It is interesting to note that the distribution ofthe species ofthe bonariensis group match- I am grateful to Cristina Scioscia who made es almost exactly three different Phytogeo- many useful suggestions for the improvement j' graphic Provinces; B. chacoensis is found al- of the first draft of the manuscript. I am also most exclusively in the “Chaquena” Province grateful to Cristina Marinone, Mark S. Harvey , with a very slight extension into the '‘Espi- and two anonymous reviewers for their help- nal” Province (Maury & Acosta 1993; Acosta ful comments. OJANGUREN AFFILASTRO—NEW SCORPION FROM ARGENTINA 61 Biodiversidad de Artropodos Argentines, Edicio- nes Sur. La Plata, Argentina. & Cabrera, A.L. A. Willink. 1980, Biogeografia de America Latina. Monografia 13. Serie Biologia, Organizacion de los Estados Americanos, Wash- ington, DC. 122 pp. Lourenpo, WR. 1993. A review ofthe geographical distribution ofthegenusAnanterisThorell (Scor- piones: Buthidae), with the description of a new species. Revista de Biologia Tropical 41:697- 701. Maury, E.A. 1973. Los escorpiones de los sistemas Serranos de laprovinciadeBuenos Aires. Physis, Buenos Aires, C 32(85):351-371. Maury, E.A. 1979. Apuntes para una zoogeografia de la escorpiofauna argentina. Acta Zoologica Lilloana 35:703-719. Maury, E.A. 1984. Redescripcion de Bothriurus Kraepelie 1911 (Scorpiones, Bothriuridae). Journal of Arachnology 12:351-356. Maury, E.A. 1986. Guia para la identificacion de los escorpiones de la provincia de Buenos Aires. Museo Argeetino de Ciencias Naturales “Ber- nardino Rivadavia”, Suplemento 1. Aracnologia, SupL 6:1-10. Maury, E.A. & L.E. Acosta. 1993. Un nuevo Both- 16 riurus del grupo bonariensis (Scorpiones, Both- — riuridae). Boletin de la Sociedad Biologica de Figure 16. Distribution ofthe species ofthebo~ Concepcion 64:113-119. nariensis group in Argentina, and the Phytogeo- Mello Leitao, C. de. 1945. Escorpioes Sul-Ameri- graphic Provinces where they have been collected. canos. Arquivos do Museu Nacional 40:7-468. Black circles: Bothriurus jesuita, black triangles: Ojanguren Affilastro, A.A. & D. Vezzani. 2001. Bothriurus chacoensis, black squares: Bothriurus Nuevoregistro deAnanterisbalzani(Scorpiones, bonariensis, hatched horizontal: “Paranaense” Buthidae) para la Argentina y ampliacion de la Phytogeographic Province; hatched vertical: “Es- distribucion geografica de Bothriurus corduben- pinal” Phytogeographic Province; darkgrey: “Cha- sis (Scorpiones, Bothriuridae). Physis, Buenos quena” Phytogeographic Province; light grey: Aires, C 58(134-135):13-14. “Pampeana” Phytogeographic Province. Stahnke, H.L. 1970. Scorpion nomenclature and mensuration. Entomological News 83:121-133. Vachon M. 1973 (1974). Etude des caracteres pour classer les families et les genres de scorpions LITERATURE CITED (Arachnides). 1. La trichobothriotaxie en Arach- Acosta, L.E. & B. Rosso de Ferradas. 1996. Arac- ncohloobgoiteh.rioStigalxeise tcrhieczholbeosthsrcioarupxioents.tyBpuelsletdientdriu- nidos de la provincia de Cordoba, In, di Tada, Museum National d’Historie Naturelle, 3e sen, LE. & E.H. Bucher (eds.), Biodiversidad de la 140, ZooL 104:857-958. Provincia de Cordoba, Fauna, 1:71-99. Acosta, L.E. & E.A. Maury. 1998. Scorpiones, pp. Manuscript received 26 April 2002, revised 8 Oc- 545-559. In Morrone, JJ. & S. Coscaroe (eds,), tober 2002.