A REVISION OF THE WORLD SPECIES OF QUADRASTICHODELLA GIRAULT, WITH Title DESCRIPTIONS OF FOUR NEW SPECIES (HYMENOPTERA,EULOPHIDAE) Author(s) IKEDA, Eiji Insecta matsumurana. Series entomology. New series, 55: 13- Citation 35 Issue Date 1999-03 Doc URL http://hdl.handle.net/2115/9890 Right Type bulletin Additional Information Instructions for use Hokkaido University Collection of Scholarly and Academic Papers : HUSCAP INSECTA MATSUMURANA NEW SERIES 55: 13-3,5 MARCH 1999 AREVISION OF THE WORLD SPECIES OF QUADRASTICHODELLA GIRAULT, WITH DESCRIPTIONS OF FOUR NEW SPECIES (HYMENOPTERA, EULOPHIDAE) EIJI IKEDA 1) Abstract IKEDA, E. 1999. A revision of the world species of Quadrastichodella Girault, with descriptions offour new species (Hymenoptera, Eulophidae). Ins. matsum. n. s. 55: 13-35,57 figs. Six species of the genus Quadrastichodella Girault, Q. nova Girault, Q. aenea Girault, Q. bella Girault, Q. boudiennyi Girault, Q. candida (Girault), Q. cyaneiviridis (Girault) are redescribed mostly on the basis of the type material. Four species, Q. neglectae sp. n. from Australia, Q. pilosa sp. n. from New Zealand, Q. gracilis sp. n. from Japan, and Q. hirsuta sp. n. from the Malay Peninsula are described. A key to the females of these species except for Q. boudiennyi Girault is provided. Two species-groups, the bella-and gracilis-groups, are proposed. Author:S address. Systematic Entomology, Faculty of Agriculture, Hokkaido University, Kita 9 Nishi 9, Kita-ku, Sapporo, 060-8589 Japan. Contents. Introduction - Genus Quadrastichodella Girault - Key to the species of Quadrastichodella Girault - Quadrastichodella nova - Q. bella - Q. aenea - Q. boudiennyi - Q. candida - Q. cyaneiviritlis - Q. neglectae sp. n. - Q. pilosa sp. n. - Q. gracilis sp. n. - Q. hirsuta sp. n. - Acknowledgements - References. 1) Research Fellow of the Japan Society for the Promotion of Science. 13 INTRODUCTION The genus Quadrastichodella was erected by Girault in 1913, and 6 species have hitherto been recognized. Members of this genus are easily diagnosed by an unique feature, the strongly raised rasp-like reticulation on the inner surfaces of the scape and pedicel. They are known to be associated with Eucalyptus and all distributed in Australia. Only Q. nova was introduced into USA, southern Europe, and South Africa with Eucalyptus (Boucek, 1977, 1988). Although the biology (Flock, 1957) and morphology (Timberlake, 1957; De Santis, 1957; LaSalle, 1994) of Q. nova have been relatively well studied, the other 5 species of this genus have not been fully examined. In this paper I redescribe the 6 known species mainly on the basis of the type material, and describe 4 new species from Australia, New Zealand, Japan, and Malaysia. The two of them which are from Japan and Malaysia are rather remarkably different from the Australasian species in their morphology. Moreover, the Japanese species is associated not with Eucalyptus but with Ulmus davidiana var. japonica. I propose 2 species-groups in Quadrasichodella on the basis of a morphological and a biological gap between the Asian and Australasian species. Specimens in the following institutions are examined (abbreviations in parentheses). Australian National Insect Collection, Canberra, Australia (ANIC). Entomological Laboratory, Faculty of Agriculture, KyfishU University, Fukuoka, Japan (ELKU). National Institute ofAgro-Environmental Sciences, Tsukuba, Ibaraki, Japan (NIAES). Queensland Museum, South Bristbane, Australia (QM). South Australian Museum, Adelaide, Australia (SAM). Systematic Entomology, Faculty of Agriculture, Hokkaido University, Sapporo, Japan (SEHU). I generally follow Graham's (1987) terminology in this study. The length of the pronotum is measured when the dorsal surface of the mesoscutum is horizontal. Genus Quadrastichodella Girault Quadrastichodella Girault, 1913b: 69.JYpe species: Quadrastichodella bella Girault (original designation). Quadrastichodes Girault, 1913b: 68. Type species: Quadrastichodes cyaneiviridis Girault (original designation). Synonymized by BotiCek, 1988: 668. Flockiella Timberlake, 1957: 109. Type species: Flockiella eucalypti Timberlake (= Q. nova Girault) (original designation). Synonymized by Boucek, 1977: 25. Diagnosis. Inner surfaces of scape and pedicel with strongly raised rasp-like reticulation (Figs. 22,23), or with many short spines instead of reticulation (Figs. 45, 46). Occiput with a transverse groove or narrow depression which is crossing foramen magnum at level of upper margin of foramen magnum (Figs. 43, 44). Antenna with 4 anelli and 3-segmented funicule in female (Figs. 7, 8), with 3 anelli and 4-segmented funicule in male (Fig. 31). Scrobal groove (Figs. 1,18) deep and smooth. Face with 2 longitudinal grooves between toruli and clypeus. Gena weakly to strongly swollen. Malar sulcus weakly to strongly curved. Anterior margin of clypeus (Figs. 3, 21) weakly bilobed. Axilla (Figs. 4,25) strongly advanced. Scutellum (Figs. 5, 26) with distinct submedian and sublaterallines. Submarginal vein of forewing (Figs. 13,32) with 2 or more dorsal setae. Postmarginal vein of forewing about half as long as stigmal vein. Cercal setae (Fig. 6) subequal in length. 14 The 2 species-groups proposed, the bella-and gracilis-groups, are diagnosed as follows: The bella-group Diagnosis. Scape and pedicel with strongly raised rasp-like reticulation on the inner surfaces. Clava 3-segrnented. Ocellar triangle (Figs. 2, 20) surrounded by groove at least anteriorly and laterally. Gena strongly swollen. Malar sulcus strongly to weakly curved. Propodeum (Figs. 5, 27) at most as long as dorsellum, usually shorter, without paraspiracular carina; spiracle large, with outer rim of spiracle covered by lobe of callus; area around propodeal spiracle at least weakly depressed and smooth. Speculum of forewing (Figs. 13, 32) open. Hind coxa very weakly engraved-reticulate, almost smooth. Petiole (Figs. 27) short and indistinct. Host. Members of the bella-group are associated with Eucalyptus, and probably phytophagous (BouCek, 1988). The gracilis-group Diagnosis. Scape and pedicel with many short spines (Figs. 45, 46) or rasp-like raised-reticulation (Fig. 55) on the inner surfaces. Clava 1-or 2-segrnented. Ocellar triangle (Figs. 41,56) not surrounded by groove. Gena (Fig. 42) weakly swollen. Malar sulcus weakly curved. Propodeum (Figs. 51,57) longer than dorsellum, with paraspiracular carina; spiracle small, with whole rim exposed. Speculum of forewing (Fig. 54) closed. Hind coxa (Figs. 51, 57) distinctly raised-reticulate. Petiole (Fig. 57) distinct, wider than long. Host. One species of this species-group, Q. gracilis sp. n., was reared from flowering branches of mmus davidiana var. japonica. DISCUSSION I place the 2 new species of the gracilis-group, Q. gracilis sp. n. and Q. hirsuta sp. n., in the genus Quadrastichodella on the basis of some characteristics common to the bella group, which are mentioned in the diagnosis of the genus. The morphological gap between the gracilis-group and the bella-group, however, is fairly large, especially in the propodeum, petiole, clava, and forewing. The features on the propodeum and petiole of the gracilis group are more similar to those seen in the genus Mischotetrastichus Graham than to those in the bella-group. Moreover, the bella-group is associated with Eucalyptus, whereas no species of Eucalyptus is distributed in Japan and Malaysia. Further investigations of the Asian fauna of Tetrastichinae are needed to confirm the position of the gracilis-group. 15 Key to the species of Quadrastichodella Girault (females) Q. boudiennyi Girault is not included in this key, because the head and antennae of the type are missing and no other specimen of this species is available. 1. Propodeum (Figs. 51, 57) longer than dorsellum, with paraspiracular carina; spiracle small, with whole rim exposed. Speculum of forewing (Fig. 54) closed. Hind coxa (Figs. 51,57) distinctly raised-reticulate. Petiole (Fig. 57) distinct (the gracilis-group) .......................... 2. Propodeum (Figs. 5, 27, 39) at most as long as dorsellum, without paraspiracular carina; spiracle moderate to large, with outer rim covered by lobe of callus. Speculum of forewing (Figs. 13, 32) open. Hind coxa smooth to only weakly engraved-reticulate. Petiole (Figs. 27, 28) indistinct (the bella-group) ........................................................................................... 3. 2. Mesoscutum (Fig. 49) with a few pairs of adnotaular setae. Callus (Fig. 51) with 2 setae. Pronotum (Fig. 49) 0.6--0.8 times as long as mesoscutum. Mouth about 1.6 times as long as malar space .................................................................................................. Q. gracilis sp. n. Mesoscutum (Fig. 57) with scattered setae on the whole surface; callus with 10 setae. Pronotum 0.3 times as long as mesoscutum. Mouth (Fig. 56) 1.1 times as long as malar space ............... . ...... .......... ...... ..... ... ................ ................................................ ....................... Q. hirsuta sp. n. 3. Clava (Figs. 7, 9) with distinct terminal spine .................................................................... 4. - Clava (Figs. 8, 11) without terminal spine .......................................................................... 5. 4. Fl (Fig. 7) 0.9-1.0 times as long as wide. ................................................ Q. nova Girault. F1 (Figs. 9, 10) 1.3-1.4 times as long as wide .......................................... Q. aenea Girault. 5. F1 (Fig. 12) 1.5-1.7 times as long as wide. Pronotum (Fig. 17) short, about 0.1 times as long as mesoscutum .................................................................................. Q. cyaneiviridis (Girault) F1 (Figs. 8, 11) at most 1.3 times as long as wide. Pronotum (Figs. 14, 15, 16,25) long, at least 0.3 times as long as mesoscutum, except for Q. pilosa sp. n. ...................... ............. 6. 6. Anterior surface of fore coxa and posterior surface of fore femur (Fig. 40) with 15-25 long setae. Femora pale brown. Pronotum (Figs. 36,37) 0.1-{).3 times as long as mesoscutum ...... ......................................................................................................................... Q. pilosa sp. n. At least posterior surface of fore femur without long setae. Femora predominantly dark brown . ............................................................................................................................................ 7. 7. Mesoscutum (Fig. 16) without median line. F1 (Fig. 11) wider than long ............................. .. ........................................................................................................... Q. candida (Girault). - Mesoscutum (Figs. 14, 15) with distinct median line. F1 (Figs. 8, 30) longer than wide ... 8. 8. Eye 1.3-1.4 times as high as malar space length. Mouth 1.1-1.3 times as long as malar space. Gaster 1.9-2.0 times as long as wide, 1.2 times as long as thorax ................... Q. bella Girault. - Eye (Figs. 18, 19) 1.6-2.0 times as high as malar space length. Mouth 1.5-1.7 times as long as malar space. Gaster (Fig. 24) 2.4-2.7 times as long as wide, 1.4-1.5 times as long as thorax .......................................................................................................... Q. neglectae sp. n. 16 The bella-group Quadrastichodella nova Girault (Figs. 1-7) Quadrastichodella nova Girault, 1922: 4~1. Flockiella eucalypti Timberlake, 1957: 110. Transferred to Quadrastichodella by Boueek, 1977: 25. Synonymized with Quadrastichodella nova by Boucek, 1988: 669. Flockiella eucalypti Timberlake; Flock, 1957: 153-155. Biology. Figs. 1-6. Quadrastichodella nova Girault, ~,specimen from California, Riverside Co., Riverside. -1. Head; 2. Vertex; 3. Mouth; 4. Thorax; 5. Scutellum, dorsellum, and propodeum; 6. Apex of gaster. 17 Quadrastichodella obscurata DeSantis, 1957: 65-68. Synonymized with Quadrastichodella eucalypti by Boucek, 1977: 25. Quadrastichodella nova Girault; Dahms, 1986: 355. - Boucek, 1988: 669. - LaSalle, 1994: 193. Diagnosis (female) FI (Fig. 7) 0.9-1.0 times as long as wide. Clava without tenninal spine. Mesoscutum (Fig. 4) with distinct median line. Propodeum (Fig. 5) with weak and indistinct median carina. Callus (Fig. 5) with 2-3 setae. Femora predominantly dark brown. Redescription Female. Body length 1.4-2.3 mm. Head and thorax dark metallic green tinged with copper and red. Gaster dark brown tinged with metallic green and copper. Scape pale brown, apically dark brown; pedicel and flagellum dark brown. Coxae dark brown; hind coxa tinged with green; femora predominantly dark brown; tibiae and tarsi pale brown; fourth tarsal segment infuscate. Wings hyaline. Head (Fig. 1) 1.0-1.1 as wide as high, 1.6-1.7 times as wide as long, 0.9-1.1 times as wide as thorax. POL 3.7-3.8 times, OOLO.8-I.0 times as long as median ocellus diameter. Ocellar triangle (Fig. 2) surrounded by groove anteriorly and laterally. Frons weakly raised or engraved-reticulate. Face smooth. Eye 1.4-1.6 times as high as malar space length. Malar sulcus strongly curved. Mouth 1.2-1.5 times as long as malar space. Antenna (Fig. 7): scape 2.6-3.0 times as long as wide, not reaching vertex; combined length of pedicel and flagellum 0.7-0.8 times as long as head width; pedicel 0.7-0.8 times as long as scape; Fl about as long as wide, 0.3-0.4 times as long as pedicel; F2 0.6-0.8 times as long as wide, 0.8-1.0 times as long as Fl; F3 0.5-0.7 times as long as wide, 0.7-1.2 times as long as F2; clava 1.7-2.3 times as long as wide, 3.0-5.0 times as long as F3, without tenninal spine. Thorax (Fig. 4) 1.4-1.6 times as long as wide, weakly engraved-reticulate. Pronotum about 0.4 times as long as mesoscutum, distinctly raised-reticulate. Mesoscutum 1.0-1.5 times as long as scutellum, sometimes weakly raised-reticulate, with distinct median line and 4-6 pairs of adnotaular setae. Scutellum (Fig. 5) about 0.9 times as long as wide; anterior pair of setae situated in or slightly before the middle of scutellum. Propodeum (Fig. 5) 0.4-0.6 times as long as dorsellum, very weakly sculptured, sometimes almost smooth, with weak and indistinct median carina and very small anteromedian fovea; diameter of spiracle about as long as propodeum; callus with 3, rarely 2, setae. Forewing 2.3-2.4 times as long as wide; submarginal vein with 3 dorsal setae; costal cell:marginal vein:stigmal vein:postmarginal vein=5.1-5.3:3.8-4.0:1.0:0.7. Gaster 1.4-2.4 times as long as wide, 1.1-1.6 times as long as thorax; apex of hypopygium situated before the middle of gaster. Male. Unknown. Type material. The holotype ( ~ ) without the head (see below) is mounted on a card and in good condition. The right forewing is separated from the body. Both antennae and fragments of the head are mounted on a slide. One antenna has the scape separated. The antennae are generally in good condition, but most parts of the head are missing. For other data, see Dahms, 1986: 355. The holotype is deposited in the Queensland Museum. Additional material examined. Australia: Queensland: Coomera river, Lamington National Park, 1200', 1 ~ ,28. V. 1966 (Z. Liepa) (ANlC). USA: California: Riverside Co., Riverside, 12 ~ ,1-7. 18 IV. 1984 (1. LaSalle), ex. seed pods Eucalyptus (QM) / 25 !f , 15-22. N. 1984 (1. LaSalle), ex. seed pods Eucalyptus (SEHU). Distribution. Australia (Queensland, Victoria) (Bourek, 1988), Argentina (De Santis, 1957), Spain, Italy, South Africa (Bourek, 1977), USA (Timberlake, 1957; LaSalle, 1994). Boutek (1988) states that Q. nova was introduced with seeds of Eucalyptus into various countries from Australia. Hosts. This species is phytophagous and makes galls in young flower buds of Eucalyptus umbellata (Timberlake, 1957) and Eucalyptus resinifera (Boucek, 1977). The biology of this species in California, USA, is described by Flock (1957). Figs. 7-13. - 7. Quadrastichodella nova Girault, !f , holotype, antenna; 8. Q. bella Girault, !f , holotype, antenna; 9. Q. aenea Girault, !f, holotype, antenna. The structures of the apex of the flagellum and the basal part of the scape were not able to be observed; 10. Ditto, '?-, antenna of the specimen examined. The pedicel has rasp-like reticulation, but it is not distinct under the microscope; 11. Q. candida (GirauJt), '?- , holotype, antenna; 12. Q. cyaneiviridis (Girault), '?-, paralectotype, antenna; 13. Ditto, '?-, lectotype, forewing. Setae on the apical half are not shown. Scale: 0.1 mm. 19 Quadrastichodella bella Girault (Figs. 8, 14) Quadrastichodella bella Girault, 1913a: 237; 1913b: 69-70. - Dahms, 1983: 120. - BouCek, 1988: 669. Diagnosis (female) F1 (Fig. 8) 1.2-1.3 times as long as wide. Clava with terminal spine. Eye 1.3-1.4 times as high as malar space length. Mouth 1.1-1.3 times as long as malar space. Mesoscutum (Fig. 14) with distinct median line. Callus with 4--6 setae. Fore coxa and femora pale brown. Gaster 1.9-2.0 times as long as wide, 1.2 times as long as thorax. Redescription Female. Body length 2.0-2.8 mm. Head dark green: vertex blackish; frons tinged with copper, in the holotype with yellow spot below median ocellus; face brownish along mouth. Thorax metallic green tinged with red and copper; tegula pale brown. Gaster dark green tinged with red and copper. Scape and pedicel pale brown; flagellum dark brown. Legs pale brown; mid coxa dark brown; hind coxa dark green; tips of tarsi infuscate. Wings hyaline. Head about as wide as high, 1.5-1.6 times as wide as long, 0.9 times as wide as thorax, weakly engraved-reticulate. POL 3.0-4.0 times as long as median ocellus diameter; OOL as long as that diameter. Ocellar triangle surrounded by groove except posteriorly. Eye 1.3-1.4 times as high as malar space length. Malar sulcus strongly curved. Mouth 1.1- 1.3 times as long as malar space. Antenna (Fig. 8): scape 2.4-2.6 times as long as wide, reaching vertex; combined length of pedicel and flagellum about as long as head width; pedicel about 0.6 times as long as scape; F1 1.2-1.3 times as long as wide, 0.5-0.6 times as long as pedicel; F2 0.7-1.0 times as long as wide, 0.7-0.8 times as long as F1; F3 0.6-0.9 times as long as wide, 0.9-1.0 times as long as F2; clava 2.4-2.6 times as long as wide, 3.3-4.3 times as long as F3, with distinct terminal spine. Thorax (Fig. 14) 1.6-1.7 times as long as wide, weakly engraved-reticulate. Pronotum 0.4-0.5 times as long as mesoscutum, distinctly engraved-reticulate. Mesoscutum 1.2-1.3 times as long as scutellum, with distinct median line. Scutellum 0.9-1.0 times as long as wide; anterior pair of scutellar setae situated before the middle. Propodeum 0.6 times as long as dorsellum, with distinct median carina; diameter of spiracle about two-thirds length of propodeum; callus with 4--6 setae. Forewing 2.1-2.5 times as long as wide; submarginal vein with 5 dorsal setae; costal cell:marginal vein:stigmal vein:postmarginal vein = 4.0- 6.1:3.3~.3:1.0:0.4-0.8. Gaster 1.9-2.0 times as long as wide, 1.2-1.7 times as long as thorax; apex of hypopygium situated slightly or distinctly behind the middle. Male. Unknown. Type material. The holotype ( !?- ) without antennae and left wings is mounted on a card, with the left forewing and both antennae mounted on a slide. All are preserved in good condition. For other data of the holotype, see Dahms, 1983: 120. The holotype is deposited in the South Australian Museum. Additional material examined. Australia: Queensland: Mt. Tambourine, 1 -'f-, (A. M. Lea) (SAM) (lacks both antennae). According to Dahms (1983), this specimen is probably the paratype; 2 miles west of Redoliffe, 1 -'f- ,23. V. 1966 (Z. Liepa) (ANIC). 20 Distribution. Australia (Queensland, New South Wales). Host. Unknown. Quadrastichodella aenea Girault (Figs. 9, 10) Quadrastichodella aenea Girault, 1913a: 237. - Dahms, 1983: 12-13. - Boueek, 1988: 669. The holotype lacks the thorax, and no other specimen is available except for one crushed head mounted on a slide; therefore, it is difficult to diagnose this species at present. However, at least in the antenna, Q. aenea has a few diagnostic characteristics as given below. Diagnosis (female) Fl (Figs. 9, 10) 1.3-1.4 times, F3 0.8-0.9 times as long as wide; F2 about as long as wide. Clava without distinct terminal spine. Redescription Female. Gaster brownish metallic green, tinged with copper and red; ovipositor sheath pale brown. Forewing hyaline. Face smooth. Vertex and frons along eye weakly engraved-reticulate. Scrobal groove smooth. Malar sulcus weakly curved. Antenna (Figs. 9, 10): scape 3.0-3.1 times as long as wide; pedicel 0.5-0.6 times as long as scape; Fll.3-1.4 times as long as wide, 0.6-0.7 times as long as pedicel; F2 about as long as wide, about 0.8 times as long as Fl; F3 0.8- 0.9 times as long as wide, 0.9-1.0 times as long as F2; clava 2.3-2.6 times as long as wide, 3.0-3.8 times as long as F3, without distinct terminal spine. Forewing: costal cell:marginal vein:stigmal vein:postmarginal vein=4.6:6.0:1.0:0.8. Gaster 1.4 mm in length, 2.7 times as long as wide. Apex of hypopygium situated in the middle of gaster. Ovipositor sheath slightly swollen apically. Male. Unknown. Type material. The gaster, one hind wing, and two legs of the holotype ( ~ ) are mounted on a card. The head crushed with an antenna is mounted on a slide. For other data, see Dahms, 1983: 12- 13. The holotype is deposited in the Queensland Museum. Additional material examined. I examined a female identified by Girault, which is deposited in the Queensland Museum. The head is mounted on a slide. It is crushed into two pieces, each of which has one antenna, are mounted on a slide. For otber data, see Dahms, 1983: 12-13. Distribution. Australia (Queensland). Host. Unknown. Quadrastichodella boudiennyi Girault (Fig. 15) Quadrastichodella nova Girault, 1929: 329-330. Preoccupied by nova Girault, 1922. Quadrastichodella boudiennyi Girault, 1937. 3pp. Replacement name. - Dahms, 1983: 146. - Dahms, 1986: 355. - Boucek, 1988: 669. Two syntypes available in this paper have no head and antenna; therefore, I could not 21